Fluorescence Polarization-Based Bioassays: New Horizons [PDF]
Sensors, 2020 Fluorescence polarization holds considerable promise for bioanalytical systems because it allows the detection of selective interactions in real time and a choice of fluorophores, the detection of which the biosample matrix does not influence; thus ...
Olga D. Hendrickson +4 more
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Monitoring Labeling Reactions Using Fluorescence Polarization
CHIMIA, 2013 Fluorescence techniques are widely applied in protein research owing to their specificity and sensitivity, but require prior fluorescent labeling.
Yvan Crittin +2 more
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Fluorescence polarization modulation super-resolution imaging provides refined dynamics orientation processes in biological samples [PDF]
Light: Science & Applications, 2022 Polarization-modulated fluorescence microscopy provides molecular orientation mapping in cells.
Sophie Brasselet
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Fluorescence-Polarization-Based Assaying of Lysozyme with Chitooligosaccharide Tracers [PDF]
BiomoleculesLysozyme is a well-known enzyme found in many biological fluids which plays an important role in the antibacterial protection of humans and animals. Lysozyme assays are used for the diagnosis of a number of diseases and utilized in immunohistochemistry ...
Liliya I. Mukhametova +7 more
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Identification of HuR-RNA Interfering Compounds by Dynamic Combinatorial Chemistry and Fluorescence Polarization. [PDF]
ACS Med Chem Lett, 2023 Volpe SD +10 more
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Biophysical Journal, 1994 The time-resolved anisotropy produced in polarized fluorescence photobleaching recovery experiments has been successfully used to measure rotational correlation times in a variety of biological systems, however the magnitudes of the reported initial anisotropies have been much lower than the theoretically predicted maximum values.
Edward H. Hellen, Thomas P. Burghardt
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A universal fluorescence polarization high throughput screening assay to target the SAM-binding sites of SARS-CoV-2 and other viral methyltransferases. [PDF]
Emerg Microbes Infect, 2023 Samrat SK +12 more
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A Fluorescence Polarization Assay for Macrodomains Facilitates the Identification of Potent Inhibitors of the SARS-CoV-2 Macrodomain. [PDF]
ACS Chem Biol, 2023 Anmangandla A +9 more
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Polarization effects on fluorescence measurements [PDF]
Journal of Research of the National Bureau of Standards Section A: Physics and Chemistry, 1975 Polarization effects on fluorescence measurements are a function of four independent variables. The first is F, the polarization ratio of the exciting light which reaches the sample. The second is r, the emission anisotropy of the sample, which is the polarization "response" of the sample to plane polarized exciting light.
E.D. Cehelnik +2 more
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Super-resolution fluorescence polarization microscopy [PDF]
Journal of Innovative Optical Health Sciences, 2018 Fluorescence polarization is related to the dipole orientation of chromophores, making fluorescence polarization microscopy possible to reveal structures and functions of tagged cellular organelles and biological macromolecules.
Karl Zhanghao +4 more
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