Results 131 to 140 of about 4,840 (179)

Noncompetitive Fluorescence Polarization Immunoassay for Protein Determination

Analytical Chemistry, 2020
Fluorescent polarization immunoassay (FPIA) is a single-step immunoassay method that is applicable to point-of-care testing; however, its applicability to large biomolecules has been restricted because ordinary FPIA is a competitive assay. Here, we report a noncompetitive FPIA using the variable domain from the heavy chain of a camelid antibody (VHH ...
Mao Fukuyama, Ayano Nakamura, Keine Nishiyama, Ayuko Imai, Manabu Tokeshi, Koji Shigemura, Akihide Hibara   +6 more
openaire   +2 more sources

Fluorescence polarization immunoassay employing immobilized antibody

Biosensors and Bioelectronics, 1991
The use of an antibody immobilized on latex or silver colloid in fluorescence polarization immunoassay (FPI) is assessed. In FPI it is possible to detect antigens of high molecular weight because the molecular weight of the antibody is effectively increased.
M, Tsuruoka, E, Tamiya, I, Karube
openaire   +2 more sources

Immunoassay methods based on fluorescence polarization

Talanta, 1989
A review on the use of fluorescence polarization in immunoassay procedures is presented. Only the determination of low molecular-weight substances, such as therapeutic agents, drugs of abuse and hormones is considered because the measured change in fluorescence polarization depends on the molecular size of the analyte.
M C, Gutierrez, A, Gomez-Hens, D, Perez-Bendito   +2 more
openaire   +2 more sources

Cyclosporine monitoring by fluorescence polarization immunoassay

Clinical Biochemistry, 1991
The monitoring of cyclosporine levels in whole blood and serum has become a routine procedure for the post-transplant management of immunosuppression. We have developed fluorescence polarization immunoassays for cyclosporine in whole blood and in serum using a monoclonal antibody. These assays are fast (20 determinations in less than 22 min), sensitive
P P, Wang, E, Simpson, V, Meucci, M, Morrison, S, Lunetta, M, Zajac, R, Boeckx   +6 more
openaire   +2 more sources

Fluorescence Polarization Immunoassay of Kanamycin

Analytical Letters, 1984
Abstract Kanamycin, an aminoglycoside antibiotic, is quantitated in human serum by a fluorescence polarization immunoassay employing a novel sulfonamido derivative of 2-naphthol-6-sulfonic acid.
Jeffrey S. O'neal, Stephen G. Schulman
openaire   +1 more source

FLUORESCENT POLARIZATION IMMUNOASSAY OF MICROCYSTIN-LR

Institute of information technology, 2021
To control the highly toxic phycotoxin microcystin-LR, which comtaminates food, a test sys-tem based on polarizing fluorescent immunoassay has been developed allowing for rapid screening testing, the detection limit of microcystin-LR is up to 7.5 ng / ml; duration of analy-sis – 10 min.
Olga Dmitrievna Hendrickson, Elena Anatolievna Zvereva, Ksenia Alekseevna Maksimova, Alexandra Pavlovna Bondarenko, Sergey Aleksandrovich Eremin   +4 more
openaire   +1 more source

Homogeneous Fluorescence Polarization Immunoassay For Eicosanoids

Proceedings of the Annual International Conference of the IEEE Engineering in Medicine and Biology Society, 1992
Homogeneous fluorescence polarization immunoassay (FPIA) was applied to the development of an immunoassay of prostaglandin E 1 (PGE 1 ) selected as a model of eicosanoids. PGE 1 was derivatized with fluoresceinthiocarbamyl ethylenediamine and purified by thin layer chromatography (TLC). The purity was checked by high performance liquid chromatography
null Matt, null Nicolas, null Donner, null Stoltz   +3 more
openaire   +2 more sources

Automated Fluorescence Polarization Immunoassay for Monitoring Vancomycin

Therapeutic Drug Monitoring, 1983
We have extended fluorescence polarization immunoassay (FPIA) technology for the measurement of drugs to include the complex amphoteric glycopeptide antibiotic vancomycin (molecular weight, 1,449). Fluorescein-labeled vancomycin was employed as a tracer, and antisera specific for vancomycin were raised in rabbits by conventional procedures.
K S, Schwenzer, C H, Wang, J P, Anhalt
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Fluorescence Polarization Immunoassays for Metal Ions

Combinatorial Chemistry & High Throughput Screening, 2003
Antibodies raised against a given metal ion complex of a polyaminopolycarboxylate chelating agent can display specificity for the immunizing chelate and, when used in conjunction with a fluorophore-labeled analog of that chelate, can form the basis for highly sensitive and specific methods for detecting that metal ion by competitive inhibition ...
openaire   +2 more sources

Fluorescence Polarization Immunoassays for Potato Glycoalkaloids

Journal of Agricultural and Food Chemistry, 1995
Fluorescence polarization immunoassays (FPIAs) were developed for potato glycoalkaloids (GAB) using a polyclonal antiserum and a monoclonal antibody (MAb). Fluorescently labeled solanidine (AMF-SOL) was synthesized by coupling 4'-(aminomethyl)fluorescein (4'-AMF) to the hemisuccinate derivative of solanidine using an active ester method.
Carrie A. Thomson, Peter Sporns
openaire   +1 more source