Results 51 to 60 of about 3,402,841 (337)

Organoids in pediatric cancer research

FEBS Letters, EarlyView.
Organoid technology has revolutionized cancer research, yet its application in pediatric oncology remains limited. Recent advances have enabled the development of pediatric tumor organoids, offering new insights into disease biology, treatment response, and interactions with the tumor microenvironment.
Carla Ríos Arceo, Jarno Drost
wiley   +1 more source

Calpain small subunit homodimerization is robust and calcium‐independent

FEBS Letters, EarlyView.
Calpains dimerize via penta‐EF‐hand (PEF) domains. Using single‐molecule force spectroscopy, we measured the strength and kinetics of PEF–PEF homodimer binding. The interaction is robust, shows a transient conformational step before dissociation, and remains largely insensitive to Ca2+.
Nesha May O. Andoy, Trina Dykstra‐MacPherson, Mathias A. Bell, Peter L. Davies, Ruby May A. Sullan   +4 more
wiley   +1 more source

Ratiometric Fluorescent Probes Based on Isosteviol with Identification of Maleic Acid in Starchy Foods

Foods
The rigid saddle-shaped framework of isosteviol provides a unique host–guest recognition cavity. For the first time, we have utilized isosteviol to construct fluorescent probes 4 and 5, achieving highly selective recognition of maleic acid and fumaric ...
Xinye Qian, Chunling Zheng, Fang Zhang
doaj   +1 more source

Continuous Fluorescence Monitoring of Rapid Cycle DNA Amplification

BioTechniques, 1997
Rapid cycle DNA amplification was continuously monitored by three different fluorescence techniques. Fluorescence was monitored by (i) the double-strand-specific dye SYBR® Green I, (ii) a decrease in fluorescein quenching by rhodamine after exonuclease ...
Carl T. Wittwer, Mark G. Herrmann, Alan A. Moss, Randy P. Rasmussen   +3 more
doaj   +1 more source

Valosin‐containing protein counteracts ATP‐driven dissolution of FUS condensates through its ATPase activity in vitro

FEBS Letters, EarlyView.
Biomolecular condensates formed by fused in sarcoma (FUS) are dissolved by high ATP concentrations yet persist in cells. Using a reconstituted system, we demonstrate that valosin‐containing protein (VCP), an AAA+ ATPase, counteracts ATP‐driven dissolution of FUS condensates through its D2 ATPase activity.
Hitomi Kimura, Shin‐ichi Tate, Kyota Yasuda   +2 more
wiley   +1 more source

Modelling fluorescence quenching in systems with restricted diffusion: applications to oligonucleotides and polypeptides [PDF]

, 2004
The objectives of the present thesis are 1) to systematically develop a series of models and theoretical expressions for the diffusion-controlled reaction, which can be used to analyze the time-resolved fluorescence data in systems where the diffusion is
Wang, Xiaojuan
core   +1 more source

Continuous Fluorescence Monitoring of Rapid Cycle DNA Amplification

BioTechniques, 2013
Rapid cycle DNA amplification was continuously monitored by three different fluorescence techniques. Fluorescence was monitored by (i) the double-strand-specific dye SYBR Green I, (ii) a decrease in fluorescein quenching by rhodamine after exonuclease ...
Carl T. Wittwer, Mark G. Herrmann, Alan A. Moss, Randy P. Rasmussen   +3 more
doaj   +1 more source

Hyperosmotic stress induces PARP1‐mediated HPF1‐dependent mono(ADP‐ribosyl)ation

FEBS Letters, EarlyView.
Sorbitol‐induced hyperosmotic stress rapidly induces reversible mono(ADP‐ribosyl)ation (MARylation) on PARP1 without the signs of genotoxic signaling. We show that PARP1 autoMARylation is HPF1 dependent and forms hydroxylamine‐resistant O‐glycosidic linkages.
Anna Georgina Kopasz, Mihály Mérey, Rebeka Vásárhelyi, Ramóna Pék, Victor Imburchia, László Henn, Adrián Kószó, Nicholas D. Lakin, Ivan Ahel, Sébastien Huet, Ágnes Czibula, Gyula Timinszky   +11 more
wiley   +1 more source

Recent Development of Lysosome-Targeted Organic Fluorescent Probes for Reactive Oxygen Species

Molecules, 2023
Reactive oxygen species (ROS) are extremely important for various biological functions. Lysosome plays key roles in cellular metabolism and has been known as the stomach of cells.
Van-Nghia Nguyen, Haidong Li
doaj   +1 more source

Design strategy for germanium-rhodamine based pH-activatable near-infrared fluorescence probes suitable for biological applications

Communications Chemistry, 2019
Fluorescence probes that can detect pH are useful tools for biological research and clinical diagnosis. Here we report pH-activatable near-infrared fluorescence probes, based on hydroxymethyl germanium-rhodamine (HMGeR), that are suitable for a range of ...
Y. Koide, Ryosuke Kojima, K. Hanaoka, Koji Numasawa, Toru Komatsu, T. Nagano, Hisataka Kobayashi, Y. Urano   +7 more
semanticscholar   +1 more source