Results 61 to 70 of about 3,402,841 (337)

An isoform of 14‐3‐3 protein regulates transbilayer lipid movement at the plasma membrane

FEBS Letters, EarlyView.
Loss of 14‐3‐3ζ in CHO cells confers resistance to exogenous phosphatidylserine (PS) and impairs endocytosis‐independent inward flip‐flop of fluorescent PS at the plasma membrane. RNAi‐mediated knockdown reproduces this defect, while no additive effect is seen in ATP11C‐deficient cells.
Akiko Yamaji‐Hasegawa, Akira Hattori, Masafumi Tsujimoto, Toshihide Kobayashi   +3 more
wiley   +1 more source

Effect of Cholesterol Anchoring Group on the Properties of G-Quadruplex-Based FRET Probes for Potassium Ion

Chemosensors, 2014
In this study we report on the spectral properties and G-quadruplex folding ability of fluorescent oligonucleotide probes modified by the attachment of a cholesterol moiety.
Angelika Swiatkowska, Bernard Juskowiak
doaj   +1 more source

Residual tail twisting in ascidian larvae is stabilized by asymmetric myofibrils that resist bilateral symmetry restoration

FEBS Letters, EarlyView.
Ascidian Ciona larvae initially show strong clockwise tail twisting, which is largely corrected during development. However, a small residual twist remains. This study shows that organized helical myofibrils in tail muscles mechanically stabilize this residual asymmetry, preventing complete restoration of bilateral symmetry and revealing how embryos ...
Yuki S. Kogure, Misaki K. Nishio, Satoru Okuda, Kohji Hotta   +3 more
wiley   +1 more source

Rapid generation of chromosome-specific alphoid DNA probes using the polymerase chain reaction [PDF]

, 1992
Non-isotopic in situ hybridization of chromosome-specific alphoid DNA probes has become a potent tool in the study of numerical aberrations of specific human chromosomes at all stages of the cell cycle. In this paper, we describe approaches for the rapid
Dunham, Ian, Cremer, Thomas, Terry Featherstone, Christoph Lengauer, Featherstone, Terry, Lan Dunham, Lengauer, Christoph, Thomas Cremer   +7 more
core   +1 more source

A Bioorthogonal Double Fluorogenic Probe to Visualize Protein–DNA Interaction

Chemosensors, 2022
Two sets of bioorthogonally applicable, double fluorogenic probes, capable of sensing DNA–protein interactions, were prepared by installing an azide or tetrazine motif onto structurally fluorogenic, DNA sensitive frames.
Attila Kormos, Alexandra Egyed, Jasmine M. Olvany, Ágnes Szatmári, Adrienn Biró, Zsóka Csorba, Péter Kele, Krisztina Németh   +7 more
doaj   +1 more source

Biophysical approaches for studying viral entry

FEBS Letters, EarlyView.
Viruses infect all living organisms and have been responsible for major epidemics and pandemics. Their ongoing evolutionary battle with host defenses creates a constant need for improved tools to study viral behavior. Advancing methods to probe viral attachment, fusion, and genome release deepen our understanding of how infections begin and support the
Inbar Yosibash, Raya Sorkin
wiley   +1 more source

Specific metaphase and interphase detection of the breakpoint region in 8q24 of burkitt lymphoma cells by triple-color fluorescence in situ hybridization [PDF]

, 1992
Triple fluorescence in situ hybridization with a plasmid DNA library from sorted human chromosomes 8 in combination with bacteriophage clones flanking the breakpoint in 8q24 of the Burkitt lymphoma cell line Jl was used for the specific delineation of ...
Thomas Ried, Peter Groitl, Wiegant, Joop, Lipp, Martin, Joop Wiegant, Ried, Thomas, Martin Lipp, Groitl, P., Cremer, Thomas, Wiegant, J., Christoph Lengauer, Raap, A.K., van der Ploeg, M., Cremer, T., Mels Van Der Ploeg, Lipp, M., Ried, T., Raap, Anton K., Van Der Ploeg, Mels, Groitl, Peter, Lengauer, C., Anton K. Raap, Lengauer, Christoph, Thomas Cremer   +23 more
core   +1 more source

Development of peptide nucleic acid probes for detection of the HER2 oncogene. [PDF]

PLoS ONE, 2013
Peptide nucleic acids (PNAs) have gained much interest as molecular recognition tools in biology, medicine and chemistry. This is due to high hybridization efficiency to complimentary oligonucleotides and stability of the duplexes with RNA or DNA.
Belhu Metaferia, Jun S Wei, Young K Song, Jennifer Evangelista, Konrad Aschenbach, Peter Johansson, Xinyu Wen, Qingrong Chen, Albert Lee, Heidi Hempel, Jinesh S Gheeya, Stephanie Getty, Romel Gomez, Javed Khan   +13 more
doaj   +1 more source

Small molecule-based ratiometric fluorescence probes for cations, anions, and biomolecules

Chemical Society Reviews, 2014
Quantitative determination of specific analytes is essential for a variety of applications ranging from life sciences to environmental monitoring. Optical sensing allows non-invasive measurements within biological milieus, parallel monitoring of multiple
Min-Hee Lee, Jong Seung Kim, J. Sessler
semanticscholar   +1 more source

Septin 9 PB domains coordinate centrosome positioning and microtubule acetylation to control epithelial polarity

FEBS Letters, EarlyView.
Septin 9 polybasic domains couple phosphoinositide‐rich membrane binding to centrosome positioning, Golgi organization, and microtubule acetylation to control epithelial polarity. Their loss disrupts this axis, causing centrosome mispositioning, Golgi fragmentation, reduced microtubule acetylation, and polarity inversion via upregulation of the ...
Ting ting Cai, Mohyeddine Omrane, Nassima Benzoubir, Didier Samuel, Ama Gassama‐Diagne   +4 more
wiley   +1 more source