Results 51 to 60 of about 98,065 (290)

Cloning, expression, purification, and structural modeling of the Chandipura virus matrix protein

FEBS Open Bio, EarlyView.
This research protocol offers a guide for the cloning, expression, and purification of the Chandipura virus matrix protein using E. coli. It also includes a step‐by‐step procedure for cloning, expressing, and conducting fluorescence imaging of GFP‐fused Chandipura virus matrix protein in mammalian cell lines.
Mariana Grieben
wiley   +1 more source

Homologous expression and purification of human HAX‐1 for structural studies

FEBS Open Bio, EarlyView.
This research protocol provides detailed instructions for cloning, expressing, and purifying large quantities of the intrinsically disordered human HAX‐1 protein, N‐terminally fused to a cleavable superfolder GFP, from mammalian cells. HAX‐1 is predicted to undergo posttranslational modifications and to interact with membranes, various cellular ...
Mariana Grieben
wiley   +1 more source

Comparative Studies on the Interaction of Cochinchinenin A and Loureirin B with Bovine Serum Albumin

Journal of Spectroscopy, 2013
This paper describes the simple, sensitive, and effective spectrophotometric methods based on ultraviolet, fluorescence and circular dichroism for revealing the interactional mechanism of Cochinchinenin A (CA) and Loureirin B (LB) with bovine serum ...
Tianming Yang, Hao Zhang, Haiyan Fu, Yuanbin She, Can Huang, Yan Hu, Liping Wang   +6 more
doaj   +1 more source

Comparison of Anionic, Cationic and Nonionic Surfactants as Dispersing Agents for Graphene Based on the Fluorescence of Riboflavin

Nanomaterials, 2017
Fluorescence quenching is a valuable tool to gain insight about dynamic changes of fluorophores in complex systems. Graphene (G), a single-layered 2D nanomaterial with unique properties, was dispersed in surfactant aqueous solutions of different nature ...
Rocío Mateos, Soledad Vera, Mercedes Valiente, Ana María Díez-Pascual, María Paz San Andrés   +4 more
doaj   +1 more source

β‐Catenin/c‐Myc Axis Modulates Autophagy Response to Different Ammonia Concentrations

Advanced Biology, Volume 9, Issue 3, March 2025.
Ammonia, detoxified by the liver into urea and glutamine, impacts autophagy differently at varying levels. Low ammonia activates autophagy via c‐Myc and β‐catenin, while high levels suppress it. Using Huh7 cells and Spf‐ash mice, c‐Myc's role in cytoprotective autophagy is revealed, offering insights into hyperammonemia and potential therapeutic ...
S. Sergio, B. Spedicato, G. Corallo, A. Inguscio, M. Greco, D. Musarò, D. Vergara, A. F. Muro, G. De Sabbata, L. R. Soria, N. Brunetti Pierri, M. Maffia   +11 more
wiley   +1 more source

Synthesis and photophysical studies on 5-ethoxycarbonyl-4-cinnamyl-6-methyl-3,4-dihydropyrimidine-2(1H)-one in various solvents

Bulletin of the Chemical Society of Ethiopia, 2009
The 5-ethoxycarbonyl-4-cinnamyl-6-methyl-3,4-dihydropyrimidine-2(1H)-one (DHPM) was synthesized via Biginelli reaction and was characterized by NMR, IR, UV−Visible absorption and fluorescence spectroscopy.
Sheela N. Lad, Umesh S. Mote, Swaminath L. Bhattar, Prashant V. Anbhule, Shivajirao R. Patil   +4 more
doaj  

Infrared Neural Stimulation Elicits Distinct Molecular Pathways in Astrocytes Based on Laser Pulse Parameters

Advanced Biology, EarlyView.
Infrared (IR) light evokes distinct calcium and water transport responses in astrocytes, depending on stimulation duration and protocol. This study uses widefield imaging and pharmacology to reveal differential engagement of astroglial signaling pathways.
Wilson R. Adams, Ana I Borrachero‐Conejo, Pratheepa Kumari Rasiah, Emanuela Saracino, Roberto Zamboni, Eric Duco Jansen, Valentina Benfenati, Anita Mahadevan‐Jansen   +7 more
wiley   +1 more source

Laboratory‐Scale Procedure for Evaluating the Flux Efficiency on Melt Cleanliness and Tensile Properties of Recycled Aluminum Alloys

Advanced Engineering Materials, EarlyView.
A laboratory‐scale procedure is developed to evaluate the efficiency of melt‐cleaning and drossing fluxes during aluminium alloy recycling, studying their effects on melt cleanliness and tensile properties of secondary foundry alloys. This work provides a practical tool for foundries and aluminium refiners to assess the efficiency of salt fluxes in ...
Veronica Milani, Giulio Timelli
wiley   +1 more source

Preliminary Study of the Interaction between Chlorogenic Acid and Myofibrillar Protein Based on Multispectral Techniques

Shipin gongye ke-ji
In this study, the binding properties and structural changes associated with the interaction of Coregonus peled myofibrillar protein (MP) and different concentrations (6, 30, 150, and 300 µmol/g) of chlorogenic acid (CA) were investigated.
Jie LI, Xin GUO, Tianshuai YUAN, Qichao QU, Xinrong ZHU, Jian ZHANG   +5 more
doaj   +1 more source

Study on Interaction between Broad Bean Phenolic Compounds and Proteins using Fluorescence Method

Czech Journal of Food Sciences, 2009
Protein-polyphenol association may affect polyphenols absorption/bioavailability from gastrointestinal tract and finally modulate their biological activities.
A. Kosińska, M. Karamać, R. Amarowicz
doaj   +1 more source