Results 91 to 100 of about 22,069 (203)

Probing Native CB₂ Receptor Mobility in Plasma Membranes of Living Cells by Fluorescence Recovery After Photobleaching. [PDF]

Chembiochem
Ciaramellano F, Leuti A, Kurtz ADE, Sarott R, Westphal M, Pfaff P, Grether U, Carreira EM, Maccarrone M, Oddi S.   +9 more
europepmc   +1 more source

Using confocal microscopy and image processing to measure properties of sub‐resolution cell structures: Application to cardiac transverse tubules

Journal of Microscopy, EarlyView.
Abstract Confocal microscopes increase the resolution of images by rejecting unwanted data from the out of focus regions of the specimen. This incurs a penalty in the recorded signal to noise ratio that may be partially ameliorated by inputting additional information during image processing.
Mark B. Cannell, Cherrie H. T. Kong
wiley   +1 more source

Fluorescence recovery after photobleaching (FRAP) in neuro v1

This protocol describes a fluorescence recovery after photobleaching (FRAP) assay used to measure the mobility of α-synuclein in synaptic boutons of cultured hippocampal neurons.
Akio Mori, Robert Edwards
openaire   +1 more source

X-Ray Photon Correlation Spectroscopy, Microscopy, and Fluorescence Recovery After Photobleaching to Study Phase Separation and Liquid-to-Solid Transition of Prion Protein Condensates. [PDF]

Bio Protoc
do Amaral MJ, Passos AR, Mohapatra S, Freire MH, Wegmann S, Cordeiro Y.   +5 more
europepmc   +1 more source

The GSK3/SHAGGY‐like OsGSK3 phosphorylates and inhibits phase separation of OsFCA at Ser‐43 and Ser‐45 to regulate brassinosteroid signaling and rice architecture

New Phytologist, EarlyView.
A working model for GLYCOGEN SYNTHASE KINASE 3‐mediated phosphorylation of FLOWERING CONTROL LOCUS A in the regulation of brassinosteroid signaling and rice growth and development. Summary Brassinosteroid (BR) signaling plays a critical role in rice (Oryza sativa L.) grain development.
Jiaqi Zhang, Fan Wang, Sijia Zhang, Qian Yu, Qimiao Dong, Jianbo Li, Xianglei Wei, Huaying Du, Ye Shen, Rong Mu, Yanxiao Jia, Jinping Cheng, Hongsheng Zhang, Ji Huang, Xiuying Gao   +14 more
wiley   +1 more source

Erythrocytes membrane fluidity changes induced by adenylyl cyclase cascade activation: study using fluorescence recovery after photobleaching. [PDF]

Eur Biophys J
Semenov AN, Lugovtsov AE, Rodionov SA, Maksimov EG, Priezzhev AV, Shirshin EA.   +5 more
europepmc   +1 more source

A Modified Cas9 Scaffold Allows Extension of the Virus‐Induced Gene Editing Technology to the Large Potyvirus Genus

Plant Biotechnology Journal, EarlyView.
ABSTRACT Plant viruses are recognized as rapid and effective vectors to deliver CRISPR‐Cas reaction components into plants, a strategy termed virus‐induced gene editing (VIGE). However, VIGE is limited by the host range of the viral vectors. Development of new viral vectors to target a broad range of plant species will potentially enable the delivery ...
Fernando Merwaiss, Verónica Aragonés, Arcadio García, José‐Antonio Daròs   +3 more
wiley   +1 more source

Quantifying Dynamics in Phase-Separated Condensates Using Fluorescence Recovery after Photobleaching. [PDF]

Biophys J, 2019
Taylor NO, Wei MT, Stone HA, Brangwynne CP.   +3 more
europepmc   +1 more source

Mitochondrial Ca2+ uniporter haploinsufficiency leads to sexually dimorphic redox imbalance and metabolic remodelling in the mouse brain

The Journal of Physiology, EarlyView.
Abstract figure legend The genetic inactivation of one Mcu allele leads to sex‐specific changes in neuronal function in adult mice, that is, the firing of action potentials and the relationship between cytosolic and mitochondrial Ca2+ levels. The ability to produce NAD(P)H by stimulated neural tissue is largely preserved in male mice but delayed in ...
Jenna Gray, Girish Halemirle, Beatriz Ferrán, Hadyn Rose, Traci L. Redwine, Sophia Pham, Bo Hagy, Atul Pranay, Jennifer Giorgione, Stacy A. Hussong, Veronica Galvan, Kenneth Humphries, Holly Van Remmen, Mike Kinter, William E. Sonntag, Pankaj K. Singh, Carlos Manlio Díaz‐García   +16 more
wiley   +1 more source

Mathematical model of the zebrafish ventricular cardiomyocyte action potential and calcium transient

The Journal of Physiology, EarlyView.
Abstract figure legend This study presents the development and validation of the first biophysically detailed computational model of the zebrafish ventricular action potential (AP). The model is based on a human cardiomyocyte framework and reparameterized using published and newly generated patch‐clamp recordings of zebrafish ionic currents.
Ludovica Cestariolo, Zachary D. Long, Arie O. Verkerk, Jose M. Ferrero, T. Alexander Quinn, Jose F. Rodriguez Matas   +5 more
wiley   +1 more source