Results 101 to 110 of about 55,338 (292)

Quantitative Fluorescence Microscopy of Protein Dynamics in Living Cells [PDF]

open access: yes, 2006
The advent of confocal microscopy, fast microcomputers with high storage capacity and, moreover, the availability of fluorescent proteins of various excitation and emission properties have made fluorescence microscopy the method of choice in the study
Ibrahim, S.M. (Shehu)
core   +1 more source

Molecular and functional characterization of gap junctions in the avian inner ear. [PDF]

open access: yes, 2006
To analyze the fundamental role of gap junctions in the vertebrate inner ear, we examined molecular and functional characteristics of gap junctional communication (GJC) in the auditory and vestibular system of the chicken.
Becker, D, Forge, A, Nickel, R
core   +1 more source

Determining Diffusion Coefficients in Inhomogeneous Tissues Using Fluorescence Recovery after Photobleaching [PDF]

open access: yesBiophysical Journal, 2005
Diffusion plays an important role in the transport of nutrients and signaling molecules in cartilaginous tissues. Diffusion coefficients can be measured by fluorescence recovery after photobleaching (FRAP). Available methods to analyze FRAP data, however, assume homogeneity in the environment of the bleached area and neglect geometrical restrictions to
Sniekers, Y.H., Donkelaar, van, C.C.
openaire   +3 more sources

Exploring the Potential of Zero‐Dimensional Carbon Nanomaterials in Photoluminescent, Electrochemiluminescent and Electrochemical Sensors

open access: yesAdvanced Materials Interfaces, EarlyView.
Zero‐dimensional carbon nanomaterials are presented as multifunctional platforms linking structure, property, and sensing performance. Surface engineering and heteroatom doping modulate electron‐transfer and luminescent behavior, enabling electrochemical, photoluminescent, and electrochemiluminescent detection. Fundamental design principles, analytical
Gustavo Martins   +8 more
wiley   +1 more source

Numerical Simulation and FRAP Experiments Show That the Plasma Membrane Binding Protein PH-EFA6 Does Not Exhibit Anomalous Subdiffusion in Cells

open access: yesBiomolecules, 2018
The fluorescence recovery after photobleaching (FRAP) technique has been used for decades to measure movements of molecules in two-dimension (2D). Data obtained by FRAP experiments in cell plasma membranes are assumed to be described through a means of ...
Cyril Favard
doaj   +1 more source

A Reusable Polymeric Sensor Based on Green‐Synthesized CQDs@ZIF‐8 for the Rapid Detection of Nitro‐Explosive Picric Acid

open access: yesAdvanced Materials Interfaces, EarlyView.
This study presents the rational design and synthesis of a highly luminescent polymeric nanocomposite. Initially, highly fluorescent carbon quantum dots (CQDs) are synthesized via a facile hydrothermal method using citric acid and β‐alanine as precursors, followed by rigorous purification through centrifugation and filtration.
Yunus Emre Toprak   +2 more
wiley   +1 more source

End‐to‐End Sensing Systems for Breast Cancer: From Wearables for Early Detection to Lab‐Based Diagnosis Chips

open access: yesAdvanced Materials Technologies, EarlyView.
This review explores advances in wearable and lab‐on‐chip technologies for breast cancer detection. Covering tactile, thermal, ultrasound, microwave, electrical impedance tomography, electrochemical, microelectromechanical, and optical systems, it highlights innovations in flexible electronics, nanomaterials, and machine learning.
Neshika Wijewardhane   +4 more
wiley   +1 more source

Analysis of Diffusion of Ras2 in Saccharomyces cerevisiae Using Fluorescence Recovery after Photobleaching

open access: yes, 2010
Binding, lateral diffusion and exchange are fundamental dynamic processes involved in protein association with cellular membranes. In this study, we developed numerical simulations of lateral diffusion and exchange of fluorophores in membranes with ...
Daniel A Beard   +10 more
core   +1 more source

Tailoring Vascular‐Immune Homeostasis via Manganese‐DNA Complex‐Armed Immunogenic Extracellular Vesicles for Pancreatic Cancer Immunotherapy

open access: yesAdvanced Science, EarlyView.
This study demonstrates that Mn2⁺–tumor DNA complexes encapsulated in dendritic cell (DC)– derived immunogenic extracellular vesicles (EVDC@Mn‐DNA) act as a DC‐specific cGAS– STING activator. EVDC@Mn‐DNA treatment enhances intratumoral DC activation, improves tumor vascular function, promotes CD8⁺ T cell activity, and suppresses pancreatic tumor growth,
Xue Jiang   +13 more
wiley   +1 more source

Tomato UDP-Glucose Sterol Glycosyltransferases: A Family of Developmental and Stress Regulated Genes that Encode Cytosolic and Membrane-Associated Forms of the Enzyme

open access: yesFrontiers in Plant Science, 2017
Sterol glycosyltransferases (SGTs) catalyze the glycosylation of the free hydroxyl group at C-3 position of sterols to produce sterol glycosides. Glycosylated sterols and free sterols are primarily located in cell membranes where in combination with ...
Karla Ramirez-Estrada   +10 more
doaj   +1 more source

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