Results 231 to 240 of about 41,336 (313)

Analyses of the Dynamic Properties of Nuclear Lamins by Fluorescence Recovery After Photobleaching (FRAP) and Fluorescence Correlation Spectroscopy (FCS). [PDF]

Methods Mol Biol, 2016
Takeshi S, Pack CG, Goldman RD.
europepmc   +1 more source

Single‐molecule validation and optimised protocols for the use of secondary nanobodies in multiplexed immunoassays

Journal of Microscopy, EarlyView.
Abstract Recently developed secondary nanobodies or single‐domain antibodies present a powerful tool for immunodetection. Unlike traditional antibodies, their monovalence enables pre‐association with primary antibodies prior to sample staining, presenting a straightforward affinity‐based antibody labelling solution.
Rebecca Saleeb, Judi O'Shaughnessy, Ryan Ferguson, Candace T. Adams, Mathew H. Horrocks   +4 more
wiley   +1 more source

Fluorescence recovery after photobleaching of centrosomal GFP::KLP-7.

, 2015
Xue Han, Adames Kelly, M. E. Sykes Ellen, Srayko Martin   +3 more
openalex   +2 more sources

Starch synthesis in Brachypodium distachyon endosperm occurs in dynamic, connected amyloplast compartments

New Phytologist, EarlyView.
Summary The morphology of starch granules is a major determinant of the functional and nutritional properties of starch and is highly variable among cereal species. Much of this morphological variation stems from differences in the spatial and temporal patterns of starch granule initiation in amyloplasts during grain development.
Lara Esch, Qi Yang Ngai, Sergio G. Lopez, J. Elaine Barclay, Inmaculada Hernández‐Pinzón, Matthew J. Moscou, David Seung   +6 more
wiley   +1 more source

Transgene‐Free, Gene‐Edited Cavendish Bananas (Musa acuminata, AAA)

Plant Biotechnology Journal, EarlyView.
ABSTRACT Global consumer acceptance of gene‐edited food crops is increasing with new breeding technologies that can modify the genome without foreign DNA integration. Here, we report an Agrobacterium‐based system for transgene‐free, gene editing of the banana cultivar, Cavendish.
Maiko Kato, Jen Kleidon, Amba Phillips, Alexandra Tabrett, Robert Harding, James Dale, Jean‐Yves Paul   +6 more
wiley   +1 more source

Tomato Spotted Wilt Virus Reprogrammes Host Glycolysis to Facilitate Proliferation by a Phase‐Separated Co‐Aggregate of Nucleocapsid Protein and Phosphoglycerate Kinase

Plant Biotechnology Journal, EarlyView.
ABSTRACT Efficient viral proliferation within the host is a critical step in pathogenicity and requires adenosine triphosphate (ATP). The replication, movement and immune evasion of many plant viruses within their hosts are associated with phase separation (PS)‐derived aggregates formed by viral components.
Guangcheng Zu, Zhifu Xing, Jiao Li, Tangbing Yang, Huan Wu, Qiangsheng Ge, Yanju Wang, Baoan Song, Runjiang Song   +8 more
wiley   +1 more source

Fluorescence recovery after photobleaching and photoconversion in multiple arbitrary regions of interest using a programmable array microscope

, 2009
Guy M. Hagen, Wouter Caarls, Keith A. Lidke, Anthony H. B. de Vries, Cornelia Fritsch, B. George Barisas, Donna J. Arndt‐Jovin, Thomas M. Jovin   +7 more
openalex   +2 more sources

Stress Granule‐Associated ZmCTU2 Confers Thermotolerance in Maize via Coordinated Regulation of Proteostasis and ROS Homeostasis

Plant Biotechnology Journal, EarlyView.
ABSTRACT The escalating global temperatures and intensifying heat stress events pose significant threats to maize productivity worldwide. Uncovering key thermotolerance genes and their functional mechanisms is thus critical for developing climate‐resilient crops.
Yufang Xu, Yudong Fan, Yulian Li, Yilin Zhang, Enpeng Liu, Ruiqi Li, Peipei Ma, Tao Li, Huiyong Zhang   +8 more
wiley   +1 more source

Mathematical model of the zebrafish ventricular cardiomyocyte action potential and calcium transient

The Journal of Physiology, EarlyView.
Abstract figure legend This study presents the development and validation of the first biophysically detailed computational model of the zebrafish ventricular action potential (AP). The model is based on a human cardiomyocyte framework and reparameterized using published and newly generated patch‐clamp recordings of zebrafish ionic currents.
Ludovica Cestariolo, Zachary D. Long, Arie O. Verkerk, Jose M. Ferrero, T. Alexander Quinn, Jose F. Rodriguez Matas   +5 more
wiley   +1 more source

Efficient in vivo targeting of the myocardial scar using Moloney murine leukaemia virus complexed with nanoparticles

The Journal of Physiology, EarlyView.
Abstract figure legend The complexation of Moloney murine leukaemia virus (MMLV) with magnetic nanoparticles (MNP) greatly enhanced the transduction of (myo)fibroblasts (FB), when applied in a magnetic field, both in vitro and in vivo in cryoinjured mice.
Timo Mohr, Miriam Schiffer, Deepak Ramanujam, Esther Carls, Callum M. Zgierski‐Johnston, Thomas Kok, Pia Niemann, Caroline Geisen, Peter Kohl, Stefan Engelhardt, Bernd K. Fleischmann, Wilhelm Roell   +11 more
wiley   +1 more source