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Geometry effects on protein mobility in a synapse. [PDF]

open access: yesBiophys J
Dannenberg S   +4 more
europepmc   +1 more source

Mapping intracellular dynamics across the whole cell with spatial statistics. [PDF]

open access: yesBiophys J
Okabe Y   +4 more
europepmc   +1 more source

Poxvirus H5 mediates the formation of liquid-liquid phase separation condensates which promote virus factory assembly. [PDF]

open access: yesPLoS Pathog
Zhu J   +11 more
europepmc   +1 more source
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Long-Term Fluorescence Recovery After Photobleaching (FRAP)

2023
Numerous models have been developed for the analysis of fluorescence recovery after photobleaching (FRAP), by which intracellular diffusion and turnover rate are quantitatively evaluated. FRAP analyses typically focus on such events that occur within several minutes, but to precisely evaluate a slow turnover rate of particularly actin stress fibers ...
Takumi, Saito   +2 more
openaire   +2 more sources

Fluorescence Recovery After Photobleaching

2011
This chapter describes the use of microscope-based fluorescence recovery after photobleaching (FRAP). To quantify the dynamics of proteins within a subcellular compartment, we first outline the general aspects of FRAP experiments and then provide a detailed protocol of how to measure and analyse the most important parameters of FRAP experiments such as
Carisey, Alex   +3 more
openaire   +5 more sources

CM‐FRAP–Continuum Mechanics‐Based Fluorescence Recovery After Photobleaching

Current Protocols, 2023
AbstractFluorescence recovery after photobleaching (FRAP) is widely used to evaluate intracellular molecular turnover or repeated translocation of molecules using confocal laser scanning microscopy. While numerous models have been developed for the analysis of FRAP responses, in which chemical interactions and/or fast diffusion processes are involved ...
Takumi, Saito, Shinji, Deguchi
openaire   +2 more sources

Toward reducing uncertainty in Fluorescence Recovery After Photobleaching

2013 35th Annual International Conference of the IEEE Engineering in Medicine and Biology Society (EMBC), 2013
We investigate the uncertainty associated with the Fluorescence Recovery After Photobleaching, FRAP, which is widely used in the determination of diffusion coefficient for bio molecules. The uncertainty of our FRAP technique stems from the measurement of the spot size and the half time.
Jeonghoon, Lee   +3 more
openaire   +2 more sources

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