Results 261 to 270 of about 40,790 (312)

POMPOMS: Crosslinked Biomolecular Condensates as a Versatile Platform for Multifunctional Protein Microparticles. [PDF]

Biomacromolecules
Park AS, Ding EA, Schuster BS.
europepmc   +1 more source

Estimation of nucleic acid mimics (NAMs) diffusion in bacteria with fluorescence recovery after photobleaching (FRAP) and PyFRAP

Beatriz T. Magalhães, Alexander Herrmann, Rita S. Santos, Nuno F. Azevedo   +3 more
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Fluorescence recovery in the super-resolution regime reveals subcompartments of 53BP1 foci. [PDF]

Cell Rep Methods
Wu C, Manjarrez-González JC, Choudhury M, Shamkhi N, Ding S, Nair VM, Draviam VM.   +6 more
europepmc   +1 more source

Investigating the lateral mobility of outer hair cell plasma membrane constituents by fluorescence recovery after photobleaching

, 2009
Louise E.O. Darling
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Fluorescence Recovery After Photobleaching

2011
This chapter describes the use of microscope-based fluorescence recovery after photobleaching (FRAP). To quantify the dynamics of proteins within a subcellular compartment, we first outline the general aspects of FRAP experiments and then provide a detailed protocol of how to measure and analyse the most important parameters of FRAP experiments such as
Carisey, Alex, Stroud, Matthew, Tsang, Ricky, Ballestrem, Christoph   +3 more
openaire   +5 more sources

Long-Term Fluorescence Recovery After Photobleaching (FRAP)

2023
Numerous models have been developed for the analysis of fluorescence recovery after photobleaching (FRAP), by which intracellular diffusion and turnover rate are quantitatively evaluated. FRAP analyses typically focus on such events that occur within several minutes, but to precisely evaluate a slow turnover rate of particularly actin stress fibers ...
Takumi, Saito, Daiki, Matsunaga, Shinji, Deguchi   +2 more
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Toward reducing uncertainty in Fluorescence Recovery After Photobleaching

2013 35th Annual International Conference of the IEEE Engineering in Medicine and Biology Society (EMBC), 2013
We investigate the uncertainty associated with the Fluorescence Recovery After Photobleaching, FRAP, which is widely used in the determination of diffusion coefficient for bio molecules. The uncertainty of our FRAP technique stems from the measurement of the spot size and the half time.
Jeonghoon, Lee, Donghee, Lee, Myoung-Ock, Cho, Jung Kyung, Kim   +3 more
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Fluorescence recovery after photobleaching of suspensions of vacuoles

Biophysical Chemistry, 1996
In this work we derive theoretical expressions for the FRAP measured on a liquid suspension of vacuoles labelled by a fluorescent probe bound to the surface membrane of these vacuoles. The bleaching laser beam creates an inhomogeneity in the surface concentration of the probe molecules.
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Optimizing Detection of Tissue Anisotropy by Fluorescence Recovery after Photobleaching

Bulletin of Mathematical Biology, 2006
Fluorescence recovery after photobleaching (FRAP) has been widely used to measure fluid flow and diffusion in gels and tissues. It has not been widely used in detection of tissue anisotropy. This may be due to a lack of applicable theory, or due to inherent limitations of the method. We discuss theoretical aspects of the relationship between anisotropy
Lubkin, S. R., Wan, X.
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Confocal Fluorescence Recovery After Photobleaching of Green Fluorescent Protein in Solution

Journal of Fluorescence, 2006
Fluorescence recovery after photobleaching (FRAP) is one of the most widely used approaches to quantitatively estimate diffusion characteristics of molecules in solution and cellular systems. In general, comparison of the diffusion times (t (1/2)) from a FRAP experiment provides qualitative estimates of diffusion rates.
Thomas J, Pucadyil, Amitabha, Chattopadhyay   +1 more
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