Results 61 to 70 of about 868,915 (328)

Interaction vesicles as emerging mediators of host‐pathogen molecular crosstalk and their implications for infection dynamics

FEBS Letters, EarlyView.
Interaction extracellular vesicles (iEVs) are hybrid vesicles formed through host‐pathogen communication. They facilitate immune evasion, transfer pathogens' molecules, increase host cell uptake, and enhance virulence. This Perspective article illustrates the multifunctional roles of iEVs and highlights their emerging relevance in infection dynamics ...
Bruna Sabatke, Izadora Volpato Rossi, Marcel I. Ramirez   +2 more
wiley   +1 more source

Fluorescence resonance energy transfer-based real-time polymerase chain reaction method without DNA extraction for the genotyping of F5, F2, F12, MTHFR, and HFE

Journal of Blood Medicine, 2014
Jordi Martinez-Serra,1 Juan Robles,2 Antoni Nicolàs,3 Antonio Gutierrez,1 Teresa Ros,1 Juan Carlos Amat,1 Regina Alemany,4 Oliver Vögler,4 Aina Abelló,2 Aina Noguera,2 Joan Besalduch1 1Department of Hematology, 2Department of Clinical ...
Martinez-Serra J, Robles J, Nicolàs A, Gutierrez A, Ros T, Amat JC, Alemany R, Vögler O, Abelló A, Noguera A, Besalduch J   +10 more
doaj  

Harnessing photoinduced electron transfer to optically determine protein sub-nanoscale atomic distances

Nature Communications, 2018
Current Förster resonance energy transfer (FRET)-based fluorescence spectroscopy methods are suffering from some limitations in the field of structural biology.
Antonios Pantazis, Karin Westerberg, Thorsten Althoff, Jeff Abramson, Riccardo Olcese   +4 more
doaj   +1 more source

An enzyme‐linked immunosorbent assay (ELISA)‐based activity assay for AMP‐activated protein kinase (AMPK)

FEBS Open Bio, EarlyView.
Measuring AMP‐activated protein kinase (AMPK) activity in vitro is crucial for testing AMPK activators or inhibitors with therapeutic potential. Here, we report an enzyme‐linked immunosorbent assay (ELISA)‐based AMPK activity assay with simple steps and high sensitivity, which offers a simple, robust, and cost‐effective alternative to traditional ...
Trezze P. Nguyen, Shangze Lyu, Yang Liu
wiley   +1 more source

Development of a DNA sensor using molecular logic gate [PDF]

J Biol Phys; 39(3) (2013) 387-394, 2014
This communication reports the increase in fluorescence resonance energy transfer (FRET) efficiency between two laser dyes in presence of Deoxyribonucleic acid (DNA). Two types of molecular logic gates have been designed where DNA acts as input signal and fluorescence intensity of different bands are taken as output signal.
arxiv   +1 more source

Spatiotemporal Control Over Protein Release from Artificial Cells via a Light‐Activatable Protease

Advanced Biology, Volume 9, Issue 5, May 2025.
Stimulus‐responsive protein release is essential for intercellular communication. Mimicking this functionality in artificial cells is promising to study the working principles of cellular signaling. Herein, an engineered light‐activatable protease is implemented in a coacervate‐based artificial cell platform to establish user‐defined spatiotemporal ...
Arjan Hazegh Nikroo, Wiggert J. Altenburg, Thijs W. van Veldhuisen, Luc Brunsveld, Jan C. M. van Hest   +4 more
wiley   +1 more source

Enhanced Mechanical Properties of Injectable Chitosan–Guar Gum Hydrogel Reinforced with Bacterial Cellulose Nanofibers for Tissue Engineering Applications

Advanced Engineering Materials, EarlyView.
This study presents the development and characterization of injectable nanocomposite hydrogels based on N‐succinyl chitosan, oxidized guar gum, and bacterial cellulose nanofibers. Emphasizing enhanced mechanical properties and biocompatibility, the hydrogels exhibit fast gelation, improved structural integrity, and reduced swelling. Their potential for
Raimundo Nonato Fernandes Moreira Filho, Pascale Chevallier, Silvia Rodriguez Fernandez, Maud Viallon, Matheus Xavier de Oliveira, Ana Lorena de Brito Soares, Diego Mantovani, Judith Pessoa de Andrade Feitosa, Rodrigo Silveira Vieira   +8 more
wiley   +1 more source

Fluorescence Resonance Energy Transfer (FRET) as Biomarkers [PDF]

Materials Today: Proceedings (2020), 2020
Applications of Fluorescence Resonance Energy Transfer (FRET) in biology have expanded tremendously in the last 25 years. This technique has become a staple technique in many biological and biophysical fields. FRET based chemical sensors and biosensors can play an important role as biomarkers.
arxiv  

Resolution of complex fluorescence spectra of lipids and nicotinic acetylcholine receptor by multivariate analysis reveals protein-mediated effects on the receptor's immediate lipid microenvironment [PDF]

PMC Biophysics 2008, 1:6, 2009
Analysis of fluorescent spectra from complex biological systems containing various fluorescent probes with overlapping emission bands is a challenging task. Valuable information can be extracted from the full spectra, however, by using multivariate analysis (MA) of measurements at different wavelengths.
arxiv   +1 more source

Gauging the Flexibility of Fluorescent Markers for the Interpretation of Fluorescence Resonance Energy Transfer

Journal of the American Chemical Society, 2010
Intramolecular distances in proteins and other biomolecules can be studied in living cells by means of fluorescence resonance energy transfer (FRET) in steady-state or pulsed-excitation experiments. The major uncertainty originates from the unknown orientation between the optical dipole moments of the fluorescent markers, especially when the molecule ...
Rindermann, Jan J., Akhtman, Yosef, Richardson, James, Brown, Tom, Lagoudakis, Pavlos G.   +4 more
openaire   +5 more sources