In vitro and in vivo validation of human and goat chondrocyte labeling by green fluorescent protein lentivirus transduction [PDF]
We investigated whether human articular chondrocytes can be labeled efficiently and for long-term with a green fluorescent protein (GFP) lentivirus and whether the viral transduction would influence cell proliferation and tissue-forming capacity.
Acharya, Chitrangada +9 more
core +1 more source
Molecular diagnosis of human metapneumovirus
Background The recent discovery of human metapneumovirus (hMPV) as a major respiratory pathogen has been made possible by means of reverse transcriptase-PCR (RT-PCR).
Essam S. Badawy +3 more
doaj +1 more source
Approaches to overcome flow cytometry limitations in the analysis of cells from veterinary relevant species [PDF]
BACKGROUND: Flow cytometry is a powerful tool for the multiparameter analysis of leukocyte subsets on the single cell level. Recent advances have greatly increased the number of fluorochrome-labeled antibodies in flow cytometry.
Debes, Gudrun F. +2 more
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The subnuclear localization of tRNA ligase in yeast [PDF]
Yeast tRNA ligase is an enzyme required for tRNA splicing. A study by indirect immune fluorescence shows that this enzyme is localized in the cell nucleus. At higher resolution, studies using indirect immune electron microscopy show this nuclear location
Abelson, John, Clark, Michael W.
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Nanomaterials for Healthcare Biosensing Applications [PDF]
In recent years, an increasing number of nanomaterials have been explored for their applications in biomedical diagnostics, making their applications in healthcare biosensing a rapidly evolving field.
Altintas, Zeynep, Pirzada, Muqsit
core +1 more source
Accurate and early diagnosis of animal rabies is critical for undertaking public health measures. Whereas the direct fluorescent antibody (DFA) technique is the recommended test, the more convenient, direct rapid immunochemistry test (dRIT), as well as ...
K. Nithin Prabhu +8 more
doaj +1 more source
Seroprevalence of dogs to Leishmania infantum antigens in the territory of Crna Gora [PDF]
Due to expansion of vector borne diseases and their importance for human health we have investigated the prevalence of antibodies to Leishmania infantum antigens in serum samples of dogs.
Pavićević Zorica +2 more
doaj +1 more source
Diagnosis of canine rabies by the direct fluorescent antibody technique in Plateau State, Nigeria
No Abstract.Nigerian Veterinary Journal Vol. 29 (2) 2008: pp.
Ehizibolo, DO +13 more
openaire +3 more sources
Detection of laser-UV microirradiation-induced DNA photolesions by immunofluorescent staining [PDF]
A low-power laser-UV microbeam of wave-length 257 nm was used for microirradiation of a small part of the nucleus of Chinese hamster cells. Following fixation in interphase or in the subsequent metaphase indirect immunofluorescent staining was performed ...
Cremer, Christoph +3 more
core +1 more source
Fluorescent and photo-oxidizing TimeSTAMP tags track protein fates in light and electron microscopy. [PDF]
Protein synthesis is highly regulated throughout nervous system development, plasticity and regeneration. However, tracking the distributions of specific new protein species has not been possible in living neurons or at the ultrastructural level ...
Butko, Margaret T +9 more
core +1 more source

