Results 31 to 40 of about 182,643 (277)

In vitro and in vivo validation of human and goat chondrocyte labeling by green fluorescent protein lentivirus transduction [PDF]

open access: yes, 2010
We investigated whether human articular chondrocytes can be labeled efficiently and for long-term with a green fluorescent protein (GFP) lentivirus and whether the viral transduction would influence cell proliferation and tissue-forming capacity.
Acharya, Chitrangada   +9 more
core   +1 more source

Molecular diagnosis of human metapneumovirus

open access: yesThe Egyptian Journal of Internal Medicine, 2012
Background The recent discovery of human metapneumovirus (hMPV) as a major respiratory pathogen has been made possible by means of reverse transcriptase-PCR (RT-PCR).
Essam S. Badawy   +3 more
doaj   +1 more source

Approaches to overcome flow cytometry limitations in the analysis of cells from veterinary relevant species [PDF]

open access: yes, 2020
BACKGROUND: Flow cytometry is a powerful tool for the multiparameter analysis of leukocyte subsets on the single cell level. Recent advances have greatly increased the number of fluorochrome-labeled antibodies in flow cytometry.
Debes, Gudrun F.   +2 more
core   +1 more source

The subnuclear localization of tRNA ligase in yeast [PDF]

open access: yes, 1987
Yeast tRNA ligase is an enzyme required for tRNA splicing. A study by indirect immune fluorescence shows that this enzyme is localized in the cell nucleus. At higher resolution, studies using indirect immune electron microscopy show this nuclear location
Abelson, John, Clark, Michael W.
core   +1 more source

Nanomaterials for Healthcare Biosensing Applications [PDF]

open access: yes, 2019
In recent years, an increasing number of nanomaterials have been explored for their applications in biomedical diagnostics, making their applications in healthcare biosensing a rapidly evolving field.
Altintas, Zeynep, Pirzada, Muqsit
core   +1 more source

Application and Comparative Evaluation of Fluorescent Antibody, Immunohistochemistry and Reverse Transcription Polymerase Chain Reaction Tests for the Detection of Rabies Virus Antigen or Nucleic Acid in Brain Samples of Animals Suspected of Rabies in India

open access: yesVeterinary Sciences, 2018
Accurate and early diagnosis of animal rabies is critical for undertaking public health measures. Whereas the direct fluorescent antibody (DFA) technique is the recommended test, the more convenient, direct rapid immunochemistry test (dRIT), as well as ...
K. Nithin Prabhu   +8 more
doaj   +1 more source

Seroprevalence of dogs to Leishmania infantum antigens in the territory of Crna Gora [PDF]

open access: yesVeterinarski Glasnik, 2014
Due to expansion of vector borne diseases and their importance for human health we have investigated the prevalence of antibodies to Leishmania infantum antigens in serum samples of dogs.
Pavićević Zorica   +2 more
doaj   +1 more source

Diagnosis of canine rabies by the direct fluorescent antibody technique in Plateau State, Nigeria

open access: yesNigerian Veterinary Journal, 2009
No Abstract.Nigerian Veterinary Journal Vol. 29 (2) 2008: pp.
Ehizibolo, DO   +13 more
openaire   +3 more sources

Detection of laser-UV microirradiation-induced DNA photolesions by immunofluorescent staining [PDF]

open access: yes, 1980
A low-power laser-UV microbeam of wave-length 257 nm was used for microirradiation of a small part of the nucleus of Chinese hamster cells. Following fixation in interphase or in the subsequent metaphase indirect immunofluorescent staining was performed ...
Cremer, Christoph   +3 more
core   +1 more source

Fluorescent and photo-oxidizing TimeSTAMP tags track protein fates in light and electron microscopy. [PDF]

open access: yes, 2012
Protein synthesis is highly regulated throughout nervous system development, plasticity and regeneration. However, tracking the distributions of specific new protein species has not been possible in living neurons or at the ultrastructural level ...
Butko, Margaret T   +9 more
core   +1 more source

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