Results 51 to 60 of about 144,270 (311)

Detection of Toxacara canis Antibodies with the Fluorescent Antibody Technique.

Experimental Biology and Medicine, 1964
SummaryThe indirect fluorescent antibody technique was used to detect antibody against Toxacara canis in 4 groups of sera. In a group of animals infected with T. canis or A. suum, T. canis antibodies appeared within 2 weeks of infection and a weak reaction with anti-Ascaris sera appeared after repeated infection.
openaire   +2 more sources

CD47 promotes mitogen‐activated protein kinase and epithelial‐to‐mesenchymal transition molecular programs to drive prometastatic phenotypes in non‐small cell lung cancer

Molecular Oncology, EarlyView.
Beyond its role in immune evasion, this study identified that CD47 drives tumor‐intrinsic signaling in non‐small cell lung cancer (NSCLC). Transcriptomic profiling and functional studies revealed that CD47 regulates cell adhesion, migration, and metastasis through an ERK–EMT signaling axis.
Asa P.Y. Lau, Lilian G. Zhai, Ryunosuke Hoshi, Zackary Rousseau, Abraam Zakhary, Yin Fang Wu, Rola M. Saleeb, Heyu Ni, Kelsie L. Thu   +8 more
wiley   +1 more source

Diagnosis of viral epidermal hyperplasia of Japanese flounder larvae by fluorescent antibody technique. [PDF]

, 1991
The first occurrence of “viral epidermal hyperplasia" of Japanese flounder larvae was recorded in Hiroshima Prefecture in 1985, and since then the same disease has been prevailing in hatchery-reared flounder in various districts of Japan.
Koh-ichiro Mori, Muroga, Kiyokuni, Toshihiro Nakai, Kiyokuni Muroga, Nakai, Toshihiro, Mori, Koh-ichiro, Takayuki Mekuchi, Mekuchi, Takayuki   +7 more
core   +1 more source

Impact of Concordance Between Antinuclear Antibody Indirect Immunofluorescence Patterns and Myositis Antibodies in Idiopathic Inflammatory Myopathies: Study Protocol

Acta Médica Portuguesa
Idiopathic inflammatory myopathies (IIM) are a heterogeneous group of systemic autoimmune rheumatic disorders in which chronic inflammation of skeletal muscle leads to muscle weakness. Many other organs, including the skin, heart, lungs, and joints, may
Cláudia Pinto Oliveira, Mariana Pereira Silva, Carolina Vilafanha, Graça Costa, Mariana Emília Santos, Maria João Gonçalves, Priscila Silva, Vânia Fernandes, Tiago Meirinhos, Catarina Almeida, Ana Rita Prata, Anabela Barcelos, Raquel Campanilho-Marques, Eduardo Dourado   +13 more
doaj   +1 more source

An improved intracellular staining protocol for efficient detection of nuclear proteins in YFP-expressing cells

BioTechniques, 2011
Intracellular staining is a widely used flow cytometry (FCM)-based technique to detect the expression of cytoslio nucleic antigens. However, intracellular staining of cells expressing cytosolic fluorescent protein (FP) markers was proven to be ...
Maria Grupillo, Robert Lakomy, Xuehui Geng, Alexis Styche, William A Rudert, Massimo Trucco, Yong Fan   +6 more
doaj   +1 more source

Heterozygous loss‐of‐function alleles associate the conserved 3′‐5′ exoribonuclease EXOSC10 with hypersensitivity to the anticancer drug 5‐fluorouracil

Molecular Oncology, EarlyView.
EXOSC10, an essential nuclear RNA exosome‐associated 3′‐5′ exoribonuclease, is inhibited by the anticancer drug 5‐fluorouracil (5‐FU), and EXOSC10 depletion increases 5‐FU sensitivity. The colon‐cancer variant EXOSC10S402T, located in a proteolysis motif, is stable and nuclear but nonfunctional in vivo.
Radhika Sain, Yann Le Page, Frédéric Percevault, Emmanuelle Becker, Luc Negroni, Almudena Fernandez, Marta Cantero, Diego Muñoz‐Santos, Lluís Montoliu, Cyrille Garnier, Michael Primig   +10 more
wiley   +1 more source

Detection of Bacterial Kidney Disease in Wild Salmonids in the Margaree River System and Adjacent Waters Using an Indirect Fluorescent Antibody Technique

, 1979
The indirect fluorescent antibody technique demonstrated the enzootic nature of bacterial kidney disease in 224 of the 605 wild salmonids sampled from the Margaree River system and some adjacent waters.
L. Marshall, C. Gallant, D. Desautels, W. D. Paterson   +3 more
core   +1 more source

Role of the CXCR4/ALK5/Smad3 Signaling Pathway in Cancer-Induced Bone Pain

Journal of Pain Research, 2020
Chong Peng,1,* Xue-Tai Chen,1,* Heng Xu,2 Li-Ping Chen,2 Wen Shen1,2 1Jiangsu Province Key Laboratory of Anesthesiology and Jiangsu Province Key Laboratory of Anesthesia and Analgesia Application Technology, Xuzhou Medical University, Xuzhou, Jiangsu ...
Peng C, Chen XT, Xu H, Chen LP, Shen W
doaj  

Electroporation of Labeled Antibodies to Visualize Endogenous Proteins and Posttranslational Modifications in Living Metazoan Cell Types

Bio-Protocol, 2018
The spatiotemporal localization of different intracellular factors in real-time and their detection in live cells are important parameters to understand dynamic protein-based processes.
Sascha Conic, Dominique Desplancq, László Tora, Etienne Weiss   +3 more
doaj   +1 more source

Cell‐cycle‐specific lesion evolution rather than inhibition of double‐strand‐break repair underpins cisplatin radiosensitization

Molecular Oncology, EarlyView.
We analyze cisplatin–DNA adducts (CDAs) and double‐strand breaks (DSBs) in a cell‐cycle‐dependent manner. We find that CDAs form similarly across all cell cycle phases. DSBs arise only in S‐phase. CDAs might not directly impair DSB repair, but S‐phase DSB lesions evolve in the presence of CDAs and disrupt repair in G2, also causing radiosensitization ...
Ye Qiu, Xixi Lin, Emil Mladenov, Veronika Mladenova, Jürgen Thomale, Ali Sak, Yao Wang, Yunxuan Deng, Eleni Gkika, Martin Stuschke, George Iliakis   +10 more
wiley   +1 more source