Results 91 to 100 of about 516,362 (300)

Studies on Fluorescent Dyes. II

open access: yesJournal of Synthetic Organic Chemistry, Japan, 1961
Syntheses and properties of bistriazinylaminostilbene derivatives have been investigated in order to select excellent fluorescent dyes for practical use. In the synthesic experiment, change of combinations of substitution groups on the triazine ring has been made and the derivatives thus obtained were tested on their solubility, acid-resistant ...
openaire   +3 more sources

Heterobifunctional Dyes: Highly Fluorescent Linkers Based on Cyanine Dyes

open access: yesChemistryOpen, 2017
AbstractHerein, we present a new synthetic route to cyanine‐based heterobifunctional dyes and their application as fluorescent linkers between polymers and biomolecules. The synthesized compounds, designed in the visible spectral range, are equipped with two different reactive groups for highly selective conjugation under physiological conditions.
Wycisk, Virginia   +6 more
openaire   +2 more sources

Single‐molecule DNA flow‐stretch assays for high‐throughput DNA–protein interaction studies

open access: yesFEBS Open Bio, EarlyView.
We describe an optimised single‐molecule DNA flow‐stretch assay that visualises DNA–protein interactions in real time. Linear DNA fragments are tethered to a surface and stretched by buffer flow for fluorescence imaging. Using λ and φX174 DNA, this protocol enhances reproducibility and accessibility, providing a versatile approach for studying diverse ...
Ayush Kumar Ganguli   +8 more
wiley   +1 more source

Design of near-infrared fluorescent bioactive conjugated functional iron oxide nanoparticles for optical detection of colon cancer

open access: yesInternational Journal of Nanomedicine, 2012
Enav Corem-Salkmon, Benny Perlstein, Shlomo MargelThe Institute of Nanotechnology and Advanced Materials, Department of Chemistry, Bar-Ilan University, Ramat-Gan, IsraelBackground: Colon cancer is one of the major causes of death in the Western world ...
Corem-Salkmon E, Perlstein B, Margel S
doaj  

Brewing yeast viability measured using a novel fluorescent dye and image cytometer

open access: yesBiotechnology & Biotechnological Equipment, 2019
This study presents an automated, image-based cytometry method for determination of total counts and viability of yeast cells by using a newly synthesized DNA fluorescent dye PO-TEDM-1 and a new Easycounter YC instrument.
Milka Atanasova   +5 more
doaj   +1 more source

Establishing an assay to evaluate d‐amino acid oxidase enzyme kinetics and inhibition using WST‐8 redox dye

open access: yesFEBS Open Bio, EarlyView.
This study investigated a novel WST‐8‐based assay for evaluating d‐Amino acid oxidase (DAO) inhibitors. We confirmed its effectiveness using known inhibitors and found that uremic toxins possess relatively weak inhibitory activity compared to existing drugs.
Kahoko Miyake   +4 more
wiley   +1 more source

Matrix metalloproteinase‐9 regulates cell adhesion and membrane protrusive activity of ovarian cancer cells

open access: yesFEBS Open Bio, EarlyView.
Matrix metalloproteinase‐9 (MMP9) drives ovarian cancer progression. Using MMP9‐null cells (M9‐KO) created from ovarian cancer cells, we found MMP9 loss did not block Epidermal Growth Factor (EGF)‐driven E‐cadherin dissolution or EMT but delayed and reduced EGF‐driven membrane protrusions. Transient MMP9 re‐expression drove membrane protrusion.
Claire Strauel   +8 more
wiley   +1 more source

Development of an Ion-Sensor using Fluorescence Resonance Energy Transfer

open access: yes, 2014
A method is presented for the sensing of ions by determining the concentration of corresponding salts (KCl, NaCl, MgCl2, CaCl2, FeCl3, FeSO4, AlCl3) in water, based on Fluorescence resonance energy transfer (FRET) process.
Arpan Datta Roy   +4 more
core   +1 more source

MiR‐513a promotes human erythroid differentiation by modulating c‐Jun

open access: yesFEBS Open Bio, EarlyView.
During early human erythropoiesis, miR‐513a promoted erythroid differentiation in primary human CD34+ hematopoietic stem‐progenitor cells and human TF‐1 erythroleukemic cells by indirectly decreasing c‐Jun and phospho‐c‐Jun expression, which are associated with increased GATA1 expression.
MinJung Kim   +11 more
wiley   +1 more source

Macropore–matrix mass transfer of reactive solutes quantified by fluorescence imaging

open access: yesVadose Zone Journal, 2020
Horizontal infiltration experiments with a fluorescent dye (e.g., Na‐fluorescein) potentially allow the quantification of the macropore–matrix mass transfer of reactive solutes through coated macropore surfaces.
Christoph Haas   +3 more
doaj   +1 more source

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