Results 31 to 40 of about 849,654 (254)
Sensitive detection of E. coli using bioconjugated fluorescent silica nanoparticles
Applied Surface Science Advances, 2021 Fluorescent dye-doped silica nanoparticles have been a major focus of research and development as potential fluorescent probes in biological systems. This is due to their strong fluorescence intensity, excellent photostability, biocompatibility, and ease
P.D. Kasun P. Ananda +3 more
doaj +1 more source
Fluorogenic and Bioorthogonal Modification of RNA Using Photoclick Chemistry
Biomolecules, 2020 A bromoaryltetrazole-modified uridine was synthesized as a new RNA building block for bioorthogonal, light-activated and postsynthetic modification with commercially available fluorescent dyes.
Katja Krell, Hans-Achim Wagenknecht
doaj +1 more source
FEBS Letters, EarlyView.We present robust protocols for the preparation of supported lipid bilayers (SLBs) incorporating either Salmonella smooth LPS or outer membrane vesicles (OMVs). We use a combination of quartz crystal microbalance with dissipation (QCM‐D) and fluorescence microscopy to both characterize the SLBs of various compositions and to probe their interactions ...
Hudson P. Pace +6 more
wiley +1 more source
Fluorescent DNA hybridization probe preparation using amine modification and reactive dye coupling
BioTechniques, 2004 Fluorescent nucleic acid hybridization probes traditionally have been generated by enzymatic incorporation of dye-labeled nucleotides, even though incorporation efficiency is low and variable from dye to dye.
W. Gregory Cox, Victoria L. Singer
doaj +1 more source
Liver organoids: modelling complexity in homeostasis and disease
FEBS Letters, EarlyView.Studying liver in vitro has been challenging because simple 2D cell cultures fail to capture liver's cellular and architectural complexity. To bridge this gap, scientists increasingly use organoids, 3D liver models which better mimic liver composition and function. This review examines recent advances in liver organoid complexity and realism, discusses
Anna M. Dowbaj, Meritxell Huch
wiley +1 more source
, 2022 Chemical fluorescence-based photonic structures as multilevel optical anticounterfeiting materials have attracted increasing attention for extended and enhanced properties, yet the construction of fluorescent metal–organic framework (MOF)-embedded ...
Yan Gao (93649) +7 more
core +2 more sources
HeliyonFluorescent dyes are commonly used as conservative groundwater tracers to track the migration of water. Over- or underestimation of important parameters such as the water flow rate can occur if the concentration of a dye is changed by unexpected ...
Ayumi Sugiyama +3 more
doaj +1 more source
Micromachines, 2022 Research relating to dielectrophoresis (DEP) has been progressing rapidly through time as it is a strong and controllable technique for manipulation, separation, preconcentration, and partitioning of protein.
Nur Shahira Abdul Nasir +8 more
doaj +1 more source
The Shewanella oneidensis Fic enzyme SoFic targets the switch‐I region of EF‐Tu for AMPylation
FEBS Letters, EarlyView.Fic enzymes mediate diverse post‐translational modifications across all domains of life, including AMPylation. Prokaryotic EF‐Tu can be AMPylated and deAMPylated by the conserved Fic enzyme SoFic. Structural and biochemical approaches were used to characterize the effect of AMPylation on EF‐Tu, SoFic's enzymatic activities, and the enzyme‐target ...
Svenja Runge +6 more
wiley +1 more source
Carboxyfluorescein diacetate succinimidyl ester fluorescent dye for cell labeling
, 2005 Our objective was to study the properties of the carboxyfluorescein diacetate succinimidyl ester (CFDA-SE) and the methodology of cell labeling using CFDA-SE fluorescent dye. First, we analyzed the kinetics of CFDA-SE fluorescent dye intensity over time.
Yan-qiu Fang +5 more
core +1 more source