Results 41 to 50 of about 513,181 (285)

Fluorescent DNA hybridization probe preparation using amine modification and reactive dye coupling

BioTechniques, 2004
Fluorescent nucleic acid hybridization probes traditionally have been generated by enzymatic incorporation of dye-labeled nucleotides, even though incorporation efficiency is low and variable from dye to dye.
W. Gregory Cox, Victoria L. Singer
doaj   +1 more source

Asymmetric patterns of gap junctional communication in developing chicken skin [PDF]

, 1993
To study the pattern of gap junctional communication in chicken skin and feather development, we injected Lucifer Yellow into single cells and monitored the transfer of the fluorescent dye through gap junctions.
Chuong, Cheng-Ming, Fraser, Scott, Serras, Florenci   +2 more
core  

Confocal and multiphoton imaging of intracellular Ca²⁺ [PDF]

, 2010
This chapter compares the imaging capabilities of a range of systems including multiphoton microscopy in regard to measurements of intracellular Ca²⁺ within living cells. In particular, the excitation spectra of popular fluorescent
Bers, Bestvater, Bliton, Bootman, Bullen, Callamaras, Cleemann, Conchello, Conchello, Cox, Denk, Diaz, Diels, Fast, Hanley, Heintzmann, Helmchen, Jares-Erijman, Kettlewell, Kim, Levitt, Lichtman, Loughrey, Maravall, Mashanov, Masters, Miyawaki, Miyawaki, Nagai, Ogden, Poenie, Sanders, Tanaami, Thomas, Tsien, Tsien, Wang, Webb, Willig, Wilson, Wokosin, Xu, Zipfel   +42 more
core   +1 more source

Spatiotemporal and quantitative analyses of phosphoinositides – fluorescent probe—and mass spectrometry‐based approaches

FEBS Letters, EarlyView.
Fluorescent probes allow dynamic visualization of phosphoinositides in living cells (left), whereas mass spectrometry provides high‐sensitivity, isomer‐resolved quantitation (right). Their synergistic use captures complementary aspects of lipid signaling. This review illustrates how these approaches reveal the spatiotemporal regulation and quantitative
Hiroaki Kajiho, Shin Morioka, Junko Sasaki, Takehiko Sasaki   +3 more
wiley   +1 more source

Identification of groundwater microbes that decrease the concentration of the conservative tracer uranine

Heliyon
Fluorescent dyes are commonly used as conservative groundwater tracers to track the migration of water. Over- or underestimation of important parameters such as the water flow rate can occur if the concentration of a dye is changed by unexpected ...
Ayumi Sugiyama, Kotaro Nakata, Shin-Ichi Hirano, Takuma Hasegawa   +3 more
doaj   +1 more source

Carbonic anhydrase inhibitor acetazolamide shifts synaptic vesicle recycling to a fast mode at the mouse neuromuscular junction [PDF]

, 2017
Acetazolamide (AZ), a molecule frequently used to treat different neurological syndromes, is an inhibitor of the carbonic anhydrase (CA), an enzyme that regulates pH inside and outside cells.
Bertone Cueto, Nicolás Iván, Cano, Raquel, Groisman, Ayelén Ivana, Mazzone, Graciela Luján, Tabares, Lucía, Uchitel, Osvaldo Daniel   +5 more
core   +1 more source

Crosstalk between the ribosome quality control‐associated E3 ubiquitin ligases LTN1 and RNF10

FEBS Letters, EarlyView.
Loss of the E3 ligase LTN1, the ubiquitin‐like modifier UFM1, or the deubiquitinating enzyme UFSP2 disrupts endoplasmic reticulum–ribosome quality control (ER‐RQC), a pathway that removes stalled ribosomes and faulty proteins. This disruption may trigger a compensatory response to ER‐RQC defects, including increased expression of the E3 ligase RNF10 ...
Yuxi Huang, Satoshi Hashimoto, Sota Ito, Chisato Kikuguchi, Miho Hoshi, Kiyoshi Yamaguchi, Yoichi Furukawa, Toru Suzuki, Toshifumi Inada   +8 more
wiley   +1 more source

Engineering of near IR fluorescent albumin nanoparticles for in vivo detection of colon cancer

Journal of Nanobiotechnology, 2012
Background The use of near-infrared (NIR) fluorescence imaging techniques has gained great interest for early detection of cancer because water and other intrinsic biomolecules display negligible absorption or autofluorescence in this region.
Cohen Sarit, Margel Shlomo
doaj   +1 more source

Evaluation of fluorescence-based viability stains in cells dissociated from scleractinian coral Pocillopora damicornis

Scientific Reports, 2022
The application of established cell viability assays such as the commonly used trypan blue staining method to coral cells is not straightforward due to different culture parameters and different cellular features specific to mammalian cells compared to ...
Liza M. Roger, Yaa Adarkwa Darko, Tytus Bernas, Frances White, Monsurat Olaosebikan, Lenore Cowen, Judith Klein-Seetharaman, Nastassja A. Lewinski   +7 more
doaj   +1 more source

Fluorescent radiation converter [PDF]

, 1981
A fluorescence radiation converter is described which includes a substantially undoped optically transparent substrate and a waveshifter coating deposited on at least one portion of the substrate for absorption of radiation and conversion of fluorescent ...
Viehmann, W.
core   +1 more source