Chemigenetic Encoding of Fluorescent Dyes Enables High‐Fidelity and Wash‐Free Imaging of Proteins in Live Cells [PDF]
Advanced ScienceFluorescent dyes are indispensable chemical tools for protein labeling, yet their utility in live‐cell imaging remains constrained by background fluorescence from off‐target interactions. A chemigenetic strategy is presented that integrates synthetic dye
Xuelian Zhou +8 more
doaj +3 more sources
Alkoxy-Substituted Quadrupolar Fluorescent Dyes
Journal of the American Chemical Society, 2022 Polar and polarizable π-conjugated organic molecules containing push-pull chromophores have been investigated extensively in the past. Identifying unique backbones and building blocks for fluorescent dyes is a timely exercise. Here, we report the synthesis and characterization of a series of fluorescent dyes containing quadrupolar A-D-A constitutions ...
Yuanning Feng +9 more
core +14 more sources
Material-Specific Determination Based on Microscopic Observation of Single Microplastic Particles Stained with Fluorescent Dyes [PDF]
Sensors, 2022 Microplastics are increasingly suspected of having serious negative effects on ecological systems and living organisms. These effects are different based on the materials of the microplastics, leading to the importance of the determination of the ...
Hiroshi Aoki
doaj +2 more sources
Application of immuno- and affinity labeling with fluorescent dyes to in-resin CLEM of Epon-embedded cells [PDF]
Heliyon, 2023 In-resin CLEM (Correlative Light and Electron Microscopy) of Epon-embedded cells involves correlating fluorescence microscopy with electron microscopy in the same Epon-embedded ultrathin section.
Isei Tanida +4 more
doaj +2 more sources
Investigating fluorescent dyes in fluorescence-assisted screenings [PDF]
Chem. Commun., 2014 Screening of bead-based peptide libraries against fluorescent dye-labeled target proteins was found to be significantly influenced by the dye characteristics. Commercially available red fluorescent dyes with net negative charges adversely showed strong interactions with library beads.
Jee, Joo-Eun +8 more
openaire +4 more sources
Exposing Pesticide Exposure Using Fluorescent Tracer Dyes
EDIS, 2008 PI-162, a 3-page illustrated fact sheet by Frederick M. Fishel, demonstrates dermal exposure for pesticide handlers under inadequate protective conditions using fluorescent dyes and blacklight. Published by the UF Department of Agronomy, May 2008.
Frederick M. Fishel
doaj +6 more sources
Bioorthogonal site-selective conjugation of fluorescent dyes to antibodies: method and potential applications. [PDF]
RSC Adv, 2022 Antibodies are immensely useful tools for biochemical research and have found application in numerous protein detection and purification methods.
Grossenbacher P +7 more
europepmc +2 more sources
Alexa Dyes, a Series of New Fluorescent Dyes that Yield Exceptionally Bright, Photostable Conjugates
Journal of Histochemistry and Cytochemistry, 1999 Alexa 350, Alexa 430, Alexa 488, Alexa 532, Alexa 546, Alexa 568, and Alexa 594 dyes are a new series of fluorescent dyes with emission/excitation spectra similar to those of AMCA, Lucifer Yellow, fluorescein, rhodamine 6G, tetramethylrhodamine or Cy3 ...
Richard P Haugland
exaly +2 more sources
Fluorescent Dyes in Hydrological Tracing: Application Methods, Ecotoxicological Effects, and Safe Application Levels [PDF]
J XenobiotCarlos Campos +3 more
openalex +2 more sources
Fluorescence and fluorescent dyes [PDF]
Physical Sciences Reviews, 2020 Abstract The handling and control of light is becoming more and more attractive in science and technology such as data processing and requires functional chromophores. As a consequence, fluorescent materials are of special importance because they allow the processing of light energy.
openaire +3 more sources