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Near-infrared (NIR) fluorescence imaging is a noninvasive technique that provides numerous advantages for the real-time in vivo monitoring of biological information in living subjects without the use of ionizing radiation. Near-infrared fluorescent (NIRF)
Chunlong Sun +4 more
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Fluorescence cell imaging and manipulation using conventional halogen lamp microscopy. [PDF]
Technologies for vitally labeling cells with fluorescent dyes have advanced remarkably. However, to excite fluorescent dyes currently requires powerful illumination, which can cause phototoxic damage to the cells and increases the cost of microscopy.
Kazuo Yamagata +8 more
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Investigating fluorescent dyes in fluorescence-assisted screenings [PDF]
Screening of bead-based peptide libraries against fluorescent dye-labeled target proteins was found to be significantly influenced by the dye characteristics. Commercially available red fluorescent dyes with net negative charges adversely showed strong interactions with library beads.
Jee, Joo-Eun +8 more
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Evaluation of chemical fluorescent dyes as a protein conjugation partner for live cell imaging.
To optimize live cell fluorescence imaging, the choice of fluorescent substrate is a critical factor. Although genetically encoded fluorescent proteins have been used widely, chemical fluorescent dyes are still useful when conjugated to proteins or ...
Yoko Hayashi-Takanaka +4 more
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Chemical tags and beyond: Live‐cell protein labeling technologies for modern optical imaging
Imaging proteins with high resolution is crucial for studying cellular physiology and pathology. Fluorescence imaging is a privileged method to visualize proteins with subcellular precision in live cells.
Dilizhatai Saimi, Zhixing Chen
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Fluorescent Probes for STED Optical Nanoscopy
Progress in developing fluorescent probes, such as fluorescent proteins, organic dyes, and fluorescent nanoparticles, is inseparable from the advancement in optical fluorescence microscopy.
Sejoo Jeong +2 more
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Modeling the uptake of fluorescent molecules into 3D cellular spheroids
Three mathematical models were developed to analyze the dynamics of fluorescent dyes penetration into 3D cellular spheroids. Two fluorescent dyes were chosen to verify mathematical models: rhodamine 6G (R6G) as a small molecule, which can freely ...
Rokas Astrauskas +4 more
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Novel application of low pH-dependent fluorescent dyes to examine colitis
Background Endoscopy capable of fluorescence observation provides histological information on gastrointestinal lesions. We explored the novel application of low pH-dependent fluorescent dyes for fluorescence observation of crypt structure and ...
Watanabe Osamu +3 more
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Computer-Assisted Design of Environmentally Friendly and Light-Stable Fluorescent Dyes for Textile Applications. [PDF]
Five potentially environmentally friendly and light-stable hemicyanine dyes were designed based on integrated consideration of photo, environmental, and computational chemistry as well as textile applications.
Chen, Guoqiang +2 more
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A library of seven novel 1,3-benzothiazole-substituted BODIPY derivatives with tunable optical properties was synthesized. The new fluorescent dyes exhibited bathochromically shifted absorptions (up to 670 nm) and emissions centered in the red and near ...
Olga Kirkilessi +5 more
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