Results 21 to 30 of about 389,213 (234)
Heliyon, 2023 In-resin CLEM (Correlative Light and Electron Microscopy) of Epon-embedded cells involves correlating fluorescence microscopy with electron microscopy in the same Epon-embedded ultrathin section.
Isei Tanida +4 more
doaj +1 more source
CHARMM-DYES: Parameterization of Fluorescent Dyes for Use with the CHARMM Forcefield [PDF]
, 2020 We present CHARMM-compatible forcefield parameters for a series of fluorescent dyes from the Alexa, Atto and Cy families, commonly used in F¨orster resonance energy transfer (FRET) experiments.
Benjamin, Ambrose +4 more
core +2 more sources
BMC Chemistry, 2020 Near-infrared (NIR) fluorescence imaging is a noninvasive technique that provides numerous advantages for the real-time in vivo monitoring of biological information in living subjects without the use of ionizing radiation. Near-infrared fluorescent (NIRF)
Chunlong Sun +4 more
doaj +1 more source
Fluorescence cell imaging and manipulation using conventional halogen lamp microscopy. [PDF]
PLoS ONE, 2012 Technologies for vitally labeling cells with fluorescent dyes have advanced remarkably. However, to excite fluorescent dyes currently requires powerful illumination, which can cause phototoxic damage to the cells and increases the cost of microscopy.
Kazuo Yamagata +8 more
doaj +1 more source
Evaluation of chemical fluorescent dyes as a protein conjugation partner for live cell imaging.
PLoS ONE, 2014 To optimize live cell fluorescence imaging, the choice of fluorescent substrate is a critical factor. Although genetically encoded fluorescent proteins have been used widely, chemical fluorescent dyes are still useful when conjugated to proteins or ...
Yoko Hayashi-Takanaka +4 more
doaj +1 more source
Chemical tags and beyond: Live‐cell protein labeling technologies for modern optical imaging
Smart Molecules, 2023 Imaging proteins with high resolution is crucial for studying cellular physiology and pathology. Fluorescence imaging is a privileged method to visualize proteins with subcellular precision in live cells.
Dilizhatai Saimi, Zhixing Chen
doaj +1 more source
Fluorescent Probes for STED Optical Nanoscopy
Nanomaterials, 2021 Progress in developing fluorescent probes, such as fluorescent proteins, organic dyes, and fluorescent nanoparticles, is inseparable from the advancement in optical fluorescence microscopy.
Sejoo Jeong +2 more
doaj +1 more source
NMR analysis of Nile Blue (C. I. Basic Blue 12) and Thionine (C. I. 52000) in solution [PDF]
, 2011 The dyes Nile Blue (C. I. Basic Blue 12, NB) and Thionine (C. I. 52000, TH) were examined in both ionic and neutral forms in different solvents using NMR and UV-visible Spectroscopy to firmly establish the structures of the molecules and to assess the ...
Salvia, Marie-Virginie +5 more
core +4 more sources
Modeling the uptake of fluorescent molecules into 3D cellular spheroids
Nonlinear Analysis, 2019 Three mathematical models were developed to analyze the dynamics of fluorescent dyes penetration into 3D cellular spheroids. Two fluorescent dyes were chosen to verify mathematical models: rhodamine 6G (R6G) as a small molecule, which can freely ...
Rokas Astrauskas +4 more
doaj +1 more source
Novel application of low pH-dependent fluorescent dyes to examine colitis
BMC Gastroenterology, 2010 Background Endoscopy capable of fluorescence observation provides histological information on gastrointestinal lesions. We explored the novel application of low pH-dependent fluorescent dyes for fluorescence observation of crypt structure and ...
Watanabe Osamu +3 more
doaj +1 more source