Chemical tags and beyond: Live‐cell protein labeling technologies for modern optical imaging
Imaging proteins with high resolution is crucial for studying cellular physiology and pathology. Fluorescence imaging is a privileged method to visualize proteins with subcellular precision in live cells.
Dilizhatai Saimi, Zhixing Chen
doaj +1 more source
Novel application of low pH-dependent fluorescent dyes to examine colitis
Background Endoscopy capable of fluorescence observation provides histological information on gastrointestinal lesions. We explored the novel application of low pH-dependent fluorescent dyes for fluorescence observation of crypt structure and ...
Watanabe Osamu +3 more
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A library of seven novel 1,3-benzothiazole-substituted BODIPY derivatives with tunable optical properties was synthesized. The new fluorescent dyes exhibited bathochromically shifted absorptions (up to 670 nm) and emissions centered in the red and near ...
Olga Kirkilessi +5 more
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Evaluation of the diet of a carabid predator using fluorescent marking of prey
We present a UV-fluorescent dye-based prey marking technique, using three different dyes, with later detection of the dyes in the guts of predators using a micro-plate fluorescent reader.
Jan OKROUHLIK, Pavel FOLTAN
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Label-free and sensitive fluorescent detection of sequence-specific single-strand DNA based on S1 nuclease cleavage effects. [PDF]
The ability to detect sequence-specific single-strand DNA (ssDNA) in complex, contaminant-ridden samples, using a fluorescent method directly without a DNA extraction and PCR step could simplify the detection of pathogens in the field and in the clinic ...
Zheng Guan +7 more
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CHARMM-DYES: Parameterization of Fluorescent Dyes for Use with the CHARMM Forcefield
We present CHARMM-compatible forcefield parameters for a series of fluorescent dyes from the Alexa, Atto and Cy families, commonly used in F¨orster resonance energy transfer (FRET) experiments.
Benjamin, Ambrose +4 more
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Comparison of the new OPS Imaging technique with intravital microscopy: Analysis of the colon microcirculation [PDF]
Background. The OPS imaging technique has been introduced for in vivo assessment of microcirculation in humans. The aim of this study was to validate the new technique against intravital fluorescence microscopy (IFM) for the visualization of colon ...
Langer, S., Biberthaler, Peter
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A beta-herpesvirus with fluorescent capsids to study transport in living cells. [PDF]
Fluorescent tagging of viral particles by genetic means enables the study of virus dynamics in living cells. However, the study of beta-herpesvirus entry and morphogenesis by this method is currently limited.
Lisa Marcinowski +37 more
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Chemical fluorescence-based dye staining for 3-dimensional histopathology analysis
Tissue clearing for 3-dimensional (3D) imaging is increasingly utilized in many biomedical applications, including the pathological examination of human biopsy specimens.
Hyun Jung Kim +3 more
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3D visualization of additive occlusion and tunable full-spectrum fluorescence in calcite
Introducing organic guests to a crystal is a convenient way to tailor its properties. Here, the authors occlude fluorescent dyes within calcite to reveal that additives can occupy distinct zones of a crystal, and strategically embed green, blue, and red ...
David C. Green +12 more
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