Results 161 to 170 of about 328,963 (317)

SmallTalk: a novel small‐sized fusion tag for peptide expression and purification

FEBS Open Bio, EarlyView.
The SmallTalk fusion tag allows for the efficient expression and purification of soluble recombinant proteins or peptides in Escherichia coli. Testing with SmallTalk‐GFP confirmed that the proteins were soluble and folded correctly, while SmallTalk‐Bin1b maintained its antimicrobial activity against various bacterial isolates. This streamlined workflow
Atika Tariq, Nestor G. Casillas‐Vega, Alma Gomez‐Loredo, Xristo Zarate   +3 more
wiley   +1 more source

Colocalization of Fluorescent Probes: Accurate and Precise Registration with Nanometer Resolution [PDF]

, 2012
L. Stirling Churchman, James A. Spudich
openalex   +1 more source

Autophagosome marker, LC3, is released extracellularly via several distinct pathways

FEBS Open Bio, EarlyView.
This study establishes a novel HiBiT‐tagging system for ultrasensitive detection of LC3, revealing multiple pathways for its extracellular secretion. It demonstrates that LC3 is released via both autophagy‐dependent and ‐independent mechanisms, including a novel route for nonlipidated LC3‐I.
Koki Saito, Masashi Arakawa, Koki Maeda, Eiji Morita   +3 more
wiley   +1 more source

A dual-responsive NIR fluorescent probe for GSH and viscosity: applications in models of cellular inflammation and apoptosis. [PDF]

RSC Adv
Li S, Xing W, Wen W, Yan M, Ren H.
europepmc   +1 more source

DDX3X induces mesenchymal transition of endothelial cells by disrupting BMPR2 signaling

FEBS Open Bio, EarlyView.
Elevated DDX3X expression led to downregulation of BMPR2, a key regulator of endothelial homeostasis and function. Our co‐immunoprecipitation assays further demonstrated a molecular interaction between DDX3X and BMPR2. Notably, DDX3X promoted lysosomal degradation of BMPR2, thereby impairing its downstream signaling and facilitating endothelial‐to ...
Yu Zhang, Jing Wang, De‐Hui Qian, Yang‐Fan Lv, Tian‐Le Cheng, Da‐Peng Wang, Jing Zhang, Ye Fan   +7 more
wiley   +1 more source

Hyperspectral Fluorescence Imaging with a New Polarity-Ultrasensitive Fluorescent Probe. [PDF]

Adv Sci (Weinh)
Zhou R, Sha H, Fu S, Liu G, Wei J, Wang C, Hou S, Lu G.   +7 more
europepmc   +1 more source

Tracking In Vivo Lipolysis of Lipid Nanocarriers Using NIR‐II Polarity‐Sensitive Fluorescent Probes [PDF]

Yuze Zhan, Zhichao Chang, Chang Liu, Ziyu Zhang, Runtong Zhang, Yi Lü, Jianping Qi, Wei Wu, Haisheng He   +8 more
openalex   +1 more source

The Fluorescent Probe Method in Investigation of the State of Erythrocyte Membranes in White Rats at Exposure to Chemical Environmental Factors [PDF]

, 2018
Оксана Анатоліївна Наконечна, Bezrodna A. I, E. M. Kornienko, T. N. Tkacheva, S. L. Efimova, E. A. Posokhov, І.Г. Максимова І.Г. Максимова   +6 more
openalex   +1 more source

ATP13A2 is involved in intracellular polyamine transport in lung epithelial cells

FEBS Open Bio, EarlyView.
Spermidine transport in lung epithelial cells involves the polyamine transporter ATP13A2. Cell proliferation is associated with the upregulation of ATP13A2. Polyamines are present in all living cells and are implicated in various crucial cellular processes such as proliferation, apoptosis and autophagy.
Yuta Hatori, Kohei Kawabata, Takanori Kubo, Takeo Kitazawa, Sae Kanai, Madoka Iwashita, Ami Hayashi, Hiroyuki Nishi, Mikihisa Takano   +8 more
wiley   +1 more source

A new multi-purpose FRET fluorescent probe for the simultaneous detection of proteases. [PDF]

RSC Adv
Jedináková P, Barbuščáková Z, Milićević D, Kubala M, Hlaváč J.   +4 more
europepmc   +1 more source