Results 91 to 100 of about 2,532,057 (298)
Liver organoids: modelling complexity in homeostasis and disease
FEBS Letters, EarlyView.Studying liver in vitro has been challenging because simple 2D cell cultures fail to capture liver's cellular and architectural complexity. To bridge this gap, scientists increasingly use organoids, 3D liver models which better mimic liver composition and function. This review examines recent advances in liver organoid complexity and realism, discusses
Anna M. Dowbaj, Meritxell Huch
wiley +1 more source
PLoS ONE, 2016 Saccharomyces cerevisiae are widely used for imaging fluorescently tagged protein fusions. Fluorescent proteins can easily be inserted into yeast genes at their chromosomal locus, by homologous recombination, for expression of tagged proteins at ...
Ryo Higuchi-Sanabria +5 more
doaj +1 more source
FEBS Letters, EarlyView.This review focuses on the role of autophagy and mitophagy in maintaining pancreatic β‐cell function and homeostasis. We discuss how genetic defects affecting these pathways contribute to the development of type 1, type 2, monogenic, and gestational diabetes. We further explore their potential as therapeutic targets. Created in BioRender.
Yunkyeong Lee +2 more
wiley +1 more source
PLoS ONE, 2018 The discovery and use of fluorescent proteins revolutionized cell biology by allowing the visualization of proteins in living cells. Advances in fluorescent proteins, primarily through genetic engineering, have enabled more advanced analyses, including ...
Laura Canty +4 more
doaj +1 more source
Golgi enzymes are retrieved from the plasma membrane to the trans‐Golgi network
FEBS Letters, EarlyView.Golgi enzymes are traditionally considered resident proteins retained within the Golgi apparatus. Here, we demonstrate that a subset transiently reaches the cell surface and is subsequently retrieved to the trans‐Golgi network via retrograde transport. Using a nanobody‐based toolkit, we uncover a dynamic trafficking cycle of several Golgi enzymes.
Dominik P. Buser, Tina Junne
wiley +1 more source
Editorial: Mechanisms of Fluorescent Proteins
Frontiers in Molecular Biosciences, 2021 Chong Fang +3 more
doaj +1 more source
Fluorescent markers in microscopy: photophysical characteristics and applications in cell biology
Acta Biologica Slovenica, 2016 In the fluorescence microscopy of biological specimens the structures in cells and tissues usually need to be labelled with various fluorescent markers.
Urban Bogataj +3 more
doaj +1 more source
Engineering novel fluorescent proteins [PDF]
, 2006 Fluorescent proteins are intrinsically fluorescent, genetically encodable tags that can be expressed in many heterologous organisms. Originally cloned from jellyfish and corals, these proteins and their engineered derivatives have become ubiquitous tools
Shaner, Nathan Christopher
core
Single-molecule spectroscopy of fluorescent proteins [PDF]
, 2008 The discovery and use of fluorescent proteins has revolutionized cellular biology. Despite the widespread use of visible fluorescent proteins as reporters and sensors in cellular environments the versatile photophysics of fluorescent proteins is still ...
Blum, Christian +3 more
core +2 more sources
, 2021 Raw data from 8 fluorescent proteins coupled to ...
Simon Monard (11415095)
core +1 more source