Results 101 to 110 of about 2,532,057 (298)

Partial depletion of plasminogen activator inhibitor‐1 decreases subcutaneous fat cell hypertrophy and liver cholesterol in high‐fat‐fed female mice

FEBS Letters, EarlyView.
Obesity raises blood levels of PAI‐1, a protein linked to metabolic dysfunction‐associated steatotic liver disease in people with obesity. In female mice fed a high‐fat diet, partially lowering PAI‐1 led to smaller subcutaneous fat cells and lower liver cholesterol, without changing body weight or insulin sensitivity.
Claudia E. Ramirez Bustamante, Robert Sharp, Pradip K. Saha, Elizabeth S. Anaya, Elizabeth L. Lieu, Evelyn L. de Groot, Pooja Patel, Mi Jeong Heo, Aaron R. Cox, Kang Ho Kim, Sean M. Hartig   +10 more
wiley   +1 more source

A method for fixing and paraffin embedding tissue to retain the natural fluorescence of reporter proteins

BioTechniques, 2015
Green fluorescent protein (GFP) and its derivatives are routinely employed as surrogate markers for gene expression and lineage tracing in genetically engineered mice.
Akifumi Nakagawa, Kate Von Alt, Keith D. Lillemoe, Carlos Fernández-del Castillo, Andrew L. Warshaw, Andrew S. Liss   +5 more
doaj   +1 more source

Genetically encoded calcium indicators for multi-color neural activity imaging and combination with optogenetics [PDF]

, 2013
Genetically encoded calcium indicators (GECIs) are powerful tools for systems neuroscience. Here we describe red, single-wavelength GECIs, RCaMPs, engineered from circular permutation of the thermostable red fluorescent protein mRuby.
Looger, Loren L, Tolö, Johan, Michael B Orger, Leon eLagnado, Tian, Lin, Pulver, Stefan R., Marvin, Jonathan S, Gottschalk, Alexander, Lin eTian, Leon Lagnado, Carreras Calderón, Nicole, Jasper eAkerboom, Kristen E Severi, Eric R Schreiter, Kristen E. Severi, Ronak ePatel, Alexander Gottschalk, Matthias ePrigge, Nicole Carreras Calderón, Kim, Douglas S, Lin Tian, Cornelia I Bargmann, Calderon, Nicole Carreras, Marvin, Jonathan S., Sebastian eKügler, Jasper Akerboom, Douglas S Kim, Douglas S. Kim, Orger, Michael B, Tsai-Wen eChen, Macklin, John J., Sebastian Wabnig, Tsai-Wen Chen, Elisabeth Fischer, Alexander eGottschalk, Elisabeth eFischer, Sarkisyan, Karen S, Marvin, Jonathan, Schreiter, Eric, Matthias Prigge, Wardill, Trevor J, Andrew eGordus, John J Macklin, Schüler, Christina, Trevor J. Wardill, Ronak Patel, Sebastian eWabnig, Severi, Kristen E., Nicole eCarreras Calderón, Severi, Kristen E, Loren L. Looger, Trevor J Wardill, Karen S. Sarkisyan, Christina Schüler, Fischer, Elisabeth, Patel, Ronak, Hegemann, Peter, Stefan R Pulver, Pulver, Stefan R, Kügler, Sebastian, Wardill, Trevor, Eric R. Schreiter, Loren L Looger, Wabnig, Sebastian, Jonathan S. Marvin, Johan Tolö, Sebastian Kügler, Schreiter, Eric R., Patel, Ronak Bhailal, Cornelia I. Bargmann, Sarkisyan, Karen S., Schueler, Christina, John J. Macklin, Andrew Gordus, Karen S Sarkisyan, Chen, Tsai-Wen, Bargmann, Cornelia I., Jonathan S Marvin, Looger, Loren L., Kim, Douglas S., Orger, Michael B., Schreiter, Eric R, Lagnado, Leon, Johan eTolö, Peter Hegemann, Orger, Michael, Prigge, Matthias, Bargmann, Cornelia I, Gordus, Andrew, Christina eSchüler, Stefan R. Pulver, Michael B. Orger, Wardill, Trevor J., Severi, Kristen, Bargmann, Cornelia, Peter eHegemann, Akerboom, Jasper, Macklin, John J   +97 more
core   +1 more source

Ligand‐dependent transcriptional heterogeneity in cell cycle gene expression delays G1/S entry

FEBS Letters, EarlyView.
EGF and HRG induce distinct G1/S progression programs in ErbB2‐amplified BT474 breast cancer cells. Despite activating the potent ErbB2–ErbB3 heterodimer, HRG does not accelerate cell‐cycle entry. Instead, EGF promotes earlier restriction‐point passage via ERK–FOS signaling, whereas HRG activates the AKT–MYC axis, driving transcriptional heterogeneity ...
Ririn Rahmala Febri, Ayaka Nagasato‐Ichikawa, Keita Iida, Shuhei Kimura, Akira Imamoto, Mariko Okada   +5 more
wiley   +1 more source

pFPL Vectors for High-Throughput Protein Localization in Fungi: Detecting Cytoplasmic Accumulation of Putative Effector Proteins

Molecular Plant-Microbe Interactions, 2015
As part of a large-scale project whose goal was to identify candidate effector proteins in Magnaporthe oryzae, we developed a suite of vectors that facilitate high-throughput protein localization experiments in fungi.
Xiaoyan Gong, Oscar Hurtado, Baohua Wang, Congqing Wu, Mihwa Yi, Martha Giraldo, Barbara Valent, Michael Goodin, Mark Farman   +8 more
doaj   +1 more source

Proteins

, 2004
Solvation energy calculation is one of the main difficulties for the estimation of protein-ligand binding free energy and the correct scoring in docking studies.
Zhou, JJ, Wang, Q, Pei, JF, Lai, LH
core   +1 more source

Emerging experimental and computational methods for studying redox‐regulated structural transitions

FEBS Letters, EarlyView.
Redox reactions can reshape proteins and alter how they behave in cells, with important consequences for health and disease. This review explores emerging experimental and computational approaches for discovering these redox‐sensitive protein switches, revealing their structural effects, and predicting their behavior, opening new opportunities to ...
Tasneem Rass, Dana Reichmann, Gábor Erdős   +2 more
wiley   +1 more source

Synergistic perspectives—How single‐molecule biophysics complement biochemical understanding

FEBS Letters, EarlyView.
In this review, we discuss how ensemble biochemistry and single‐molecule approaches are complementary, outline commonly used single‐molecule techniques, and illustrate their relevance through two representative case studies: chromatin organization by SMC complexes and pathway choice during DNA double‐strand break repair.
Sara De Bragança, Eline L. Jansen, Jorine M. Eeftens   +2 more
wiley   +1 more source

Translophagy—A potential link between autophagy impairment and translational errors

FEBS Letters, EarlyView.
Neurodegenerative diseases are characterised by the accumulation of abnormal proteins and protein aggregates, but their origin often remains unknown. We propose that selective autophagy removes damaged protein‐making machinery, preventing errors during protein synthesis.
Mykola V. Korolchuk, Gailing Ma, Robin Antrobus, Diego Manni, Marco Trevisan‐Herraz, Mustafa Khwaja, Max Brown, Gavin D. Richardson, Masaaki Komatsu, Daniel Erskine, Viktor I. Korolchuk, Tetsushi Kataura   +11 more
wiley   +1 more source

Engineered fluorescent proteins illuminate the bacterial periplasm

Computational and Structural Biotechnology Journal, 2012
The bacterial periplasm is of special interest whenever cell factories are designed and engineered. Recombinantely produced proteins are targeted to the periplasmic space of Gram negative bacteria to take advantage of the authentic N-termini, disulfide ...
Thorben Dammeyer, Philip Tinnefeld
doaj   +3 more sources