Results 171 to 180 of about 45,567 (210)

Human Karyotyping by Heat-Giemsa Staining and Comparison with Fluorochrome Techniques

Nature New Biology, 1971
CHARACTERISTIC patterns resembling those revealed by the fluorochrome techniques1 appear along human mitotic metaphase chromosomes when methanol-acetic acid-fixed preparations are heated to 69° C and stained with Giemsa solution. This procedure is a greatly simplified version of one developed by F. E. Arrighi and T. C.
B, Lomholt, J, Mohr
openaire   +2 more sources

Characterization of the selective staining of DNA on gels using two fluorochromes

Electrophoresis, 2000
Ethidium bromide (EB) and 4'-6-diamidino-2-phenylindole (DAPI) are both well-known fluorochromes for detecting DNA fragments. EB binds to DNA by intercalation and DAPI binds in the DNA minor groove. We previously developed a staining method using both EB and DAPI that is selective for AT-rich DNA fragments.
T, Mabuchi, S, Nishikawa
exaly   +3 more sources

Late replicating bands of human chromosomes demonstrated by fluorochrome and Giemsa staining

Human Genetics, 1975
The addition of thymidine (TdR) to cells growing in a medium containing 5-bromodeoxyuridine (BUdR) at the end of the first replication cycle results in the incorporation of TdR into the late replicating DNA regions. These sites can be visualized by staining the metaphase chromosomes with the fluorescent dye "33258 Hoechst" or a "33258 Hoechst" Giemsa ...
K H, Grzeschik, M A, Kim, R, Johannsmann
openaire   +2 more sources

Linkage Groups II and XII of the Mouse: Cytological Localization by Fluorochrome Staining

Science, 1971
The T(2;12) 163H translocation involves two chromosomes which carry the two groups of genes known as linkage groups II and XII. Staining with quinacrine mustard reveals that these chromosomes are numbers 10 and 19 of the fluorescent karyotype of the normal mouse.
Nesbitt, M, Francke, U
openaire   +2 more sources

Demonstration of Mycobacteria: a Brief Review with Special Emphasis on Fluorochrome Staining

Journal of Histotechnology, 1991
exaly   +2 more sources

Stabilization of Fluorochromes After Immunofluorescent Staining of Tissue and Embedding in Epon

Stain Technology, 1986
Tissue blocks or sections immunofluorescent stained before embedding, i.g., liver and kidney, can be stored for more than 3 years without demonstrable fluorescence decay. The processing steps, including poststaining dehydration by alcohols and embedding in expoxy resins, seem to stabilize the fluorochromes fluorescein isothiocyanate (FITC) and ...
openaire   +2 more sources

Photochemical fluorochrome staining for detection of prenecrotic changes in cardiomyocyte myofibrils

Bulletin of Experimental Biology and Medicine, 1999
Ischemic and metabolic damages to cardiomyocytes induce immediate ultrastructural changes in their myofibrils. Photochemical fluorochromize staining of prenecrotic cardiomyocytes allows to determine with high sensitivity local redistribution of sarcomere mass.
L. M. Nepomnyashchikh, V. G. Tsimmerman
openaire   +1 more source

Differentiation between Viable and Dead Cryptosporidium Oocysts Using Fluorochrome Staining.

The Kobe journal of medical sciences, 2015
The use of nucleic acid staining with a fluorochrome dye to differentiate viable and dead (heat-killed) Cryptosporidium oocysts was assessed. The specificities (percentage of unstained viable oocysts) and sensitivities (percentage of stained dead oocysts) of the seven tested dyes (SYTO-17® and SYTO-59® to 64®) ranged from 65 to 76% (average 71%) and 83
Tomonaga, Tatsuya, Rai, Shiba Kumar, Uga, Shoji   +2 more
openaire   +2 more sources

Autofluorescence and fluorochrome-staining of Hassall's bodies in rabbits.

Revista brasileira de pesquisas medicas e biologicas, 1979
The phenomenon of autofluorescence is reported in the thymus of rabbits; besides reticular epithelial cells, Hassall's bodies also emit autofluorescence. The staining of thymic corpuscle by fluorescein isothiocyanate is also described. This staining is important not only because FITC is commonly used in immunofluorescence and may lead to ...
W, Savino, G L, Santa-Rosa
openaire   +1 more source

Fluorochrome Staining of Mycobacteria

American Journal of Clinical Pathology, 1966
O L, Weiser, E F, Sproat, J D, Hakes, W C, Morse   +3 more
openaire   +2 more sources