Results 31 to 40 of about 45,567 (210)

Rapid Hybridoma Screening Method for the Identification of Monoclonal Antibodies to Low-Abundance Cytoplasmic Proteins

BioTechniques, 1998
Screening assays are the most time-consuming and labor-intensive part of generating monoclonal antibodies (MAbs). Antibodies identified by enzyme-linked immunosorbent assay (ELISA) screening often are not suitable for their intended application such as ...
L.A. O’Reilly, L. Cullen, K. Moriishi, L. O’Connor, D.C.S. Huang, A. Strasser   +5 more
doaj   +1 more source

Matrix Stiffness Enhances Odontogenic Differentiation of DPSCs through Membrane Curvature Protein Baiap2‐Modulated Exosome Release

Advanced Science, EarlyView.
Matrix stiffness softening is identified as a previously underappreciated feature in deep caries. Higher stiffness drives exosome release in dental pulp stem cells through a coordinated mechanism involving PI3K–AKT‐regulated Baiap2 and kinesin‐1, thereby promoting odontogenic differentiation.
Bilun Jin, Shaojie Dong, Yue Jia, Zhao Xu, Peiqi Liu, Zhaojing Ding, Yuxin Liao, Rui Zou, Jinsong Hu, Bo Cheng, Lin Niu   +10 more
wiley   +1 more source

Comparison of Flurochrome , Ziehl-Neelsen and Tan Thiam Hok Staining Methods for Detection of Mycobacterium tuberculosis in Sputums of Patients with Pulmonary Tuberculosis

Medical Laboratory Journal, 2011
Background and objectives: Direct smear microscopy, because of its simplicity, rapidity, low cost and relatively sensitive is a suitable method to detecting pulmonary tuberculosis.
Pourhajibagher M ( Msc), Nasrollahi M(PhD)   +1 more
doaj  

Chromosomal characterization mediated by karyomorphological analysis and differential banding pattern in fenugreek (Trigonella foenum-graecum L.): a neglected legume

Caryologia
Fenugreek or Trigonella foenum-graecum L. is a commercially important yet neglected crop of the family Fabaceae, with potent medicinal applications, and can treat several diseases as well.
Indranil Santra, Diptesh Biswas, Biswajit Ghosh   +2 more
doaj   +1 more source

Rhoifolin Alleviates Ulcerative Colitis by Targeting NMNAT1 and Activating SIRT1‐FOXO1 Signaling to Enhance ILC3s Effector Function

Advanced Science, EarlyView.
Rhoifolin directly targets NMNAT1, thereby activating nicotinamide salvage pathway and promoting intracellular NAD+ biosynthesis. Elevated NAD+ levels enhance SIRT1 activity, leading to FOXO1 deacetylation and nuclear translocation. Nuclear FOXO1 subsequently binds to the IL‐22 promoter, upregulating IL‐22 transcription and potentiating ILC3 effector ...
Hongqiong Yang, Yishu Zhang, Sijia Feng, Xiaoqing Ma, Jiayi Feng, Junwei Wang, Jialin Gao, An Pan, Dapeng Wu, Kang Ding, Caijuan Zheng, Qi Lv, Lihong Hu   +12 more
wiley   +1 more source

Stress Hyperglycemia Drives CD4+ T Cell PANoptosis and Postoperative Organ Injury via Monocyte‐Derived Succinate

Advanced Science, EarlyView.
High glucose is linked to reduced succinate dehydrogenase activity in CD14+ monocytes, accompanied by succinate accumulation and extracellular release. Extracellular succinate exacerbates mitochondrial ROS production and mtDNA release in CD4+ T cells. Cytosolic mtDNA then activates Z‐DNA binding protein 1 (ZBP1) and engages ZBP1‐associated inflammatory
Shuai Zhao, Xuemei Miao, Wei Luo, Tao Liu, Yang Wang, Jiaying Li, Di Fu, Weiyun Shen, Yan Luo, Kang Chen, Ruping Dai, Hui Li   +11 more
wiley   +1 more source

Rapid Profiling Cell Cycle by Flow Cytometry Using Concurrent Staining of DNA and Mitotic Markers

Bio-Protocol, 2017
The flow cytometric quantitation of DNA content by DNA-binding fluorochrome, propidium iodide (PI) is the most widely used method for cell cycle analysis. However, the commonly used methods are time-consuming and labor-intensive and are incompatible with
Yuqing Shen, Paolo Vignali, Ruoning Wang   +2 more
doaj   +1 more source

Molecular cytogenetics insights in two pelagic big-game fishes in the Atlantic, the tarpon, Megalops atlanticus (Elopiformes: Megalopidae), and the sailfish, Istiophorus platypterus (Istiophoriformes: Istiophoridae)

Neotropical Ichthyology, 2021
Some pelagic and usually large sized fishes are preferential targets for sport and commercial fishing. Despite their economic importance, cytogenetic data on their evolutionary processes and management are very deficient, especially due to logistical ...
Rodrigo Xavier Soares, Gideão Wagner Werneck Félix da Costa, Marcelo de Bello Cioffi, Luiz Antonio Carlos Bertollo, Clóvis Coutinho da Motta-Neto, Wagner Franco Molina   +5 more
doaj   +1 more source

Engineered Xenogeneic Bone Scaffold with IL‐10 Nanodelivery System: Immunomodulation and BMSC Fate Programming for Skull Defect Repair

Advanced Science, EarlyView.
A multifunctional regenerative composite is constructed by 3D‐printing thermosensitive PNIPAM hydrogel embedded with MPDA@IL‐10 nanoparticles and autologous BMSCs onto antigen‐depleted porcine bone matrix for beagle critical‐sized skull defect reconstruction.
Weihao Lv, Lei Zhang, Fei Fei, Zhibin Wu, Xiuquan Wu, Ya‐nan Dou, Yihao Fu, Hao Chang, Yujie Qiang, Huaxia Xie, Ni An, Leiying Chen, Hongchen Zhang, Min‐Wen Zheng, Hongqing Chen, Xia Li, Zhou Fei   +16 more
wiley   +1 more source

Alternative Method to Detect Neuronal Degeneration and Amyloid β Accumulation in Free-Floating Brain Sections With Fluoro-Jade

ASN Neuro, 2018
Fluoro-Jade is a fluorescein-derived fluorochrome which specifically binds to damaged neurons. Due to this characteristic, it is commonly used for the histochemical detection and quantification of neurodegeneration in mounted brain sections.
Irene L. Gutiérrez, Marta González-Prieto, Borja García-Bueno, Javier R. Caso, Juan C. Leza, José L. M. Madrigal   +5 more
doaj   +1 more source