Results 1 to 10 of about 15,492 (288)

Conjugation of Fluorochromes to Monoclonal Antibodies [PDF]

open access: greenCurrent Protocols, 2023
AbstractDetection of cell surface molecules labeled by monoclonal or polyclonal antibodies conjugated to a fluorochrome is the most widely used application of flow cytometry. Here, we present protocols for tagging monoclonal antibodies with fluorescein, biotin, Texas Red, and phycobiliproteins.
Larry Lantz   +2 more
openalex   +4 more sources

Staining autoradiographs with the fluorochrome “33258” [PDF]

open access: bronzeThe Science of Nature, 1976
feuchtigkeitsempfindlich. Von der IridiumVerbindung konnten durch 800stfindiges Tempern bei 1000 ~ Einkristalle erhalten werden. In Tabelle 1 sind neben den pyknometrisch besfimmten Dichten die r6ntgenographischen Daten angegeben, die beim Lil,e3IrB2 aus Pr~izessionsaufnahmen, bei den beiden anderen Verbindungen aus Guinier-Aufnahmen ermittelt wurden ...
Gerd M. Seibold
openalex   +4 more sources

Flow cytometric discrimination of seven lineage markers by using two fluorochromes. [PDF]

open access: goldPLoS ONE, 2017
Flow cytometry is the primary immunological technique used to analyze multiple parameters on complex cell populations. We present a staining method that identifies major human mononuclear lymphoid and myeloid populations (CD4+ and CD8+ T cells, γδ T ...
Francesco Boin   +6 more
doaj   +3 more sources

Development of a customizable mouse backbone spectral flow cytometry panel to delineate immune cell populations in normal and tumor tissues [PDF]

open access: yesFrontiers in Immunology
IntroductionIn vivo studies of cancer biology and assessment of therapeutic efficacy are critical to advancing cancer research and ultimately improving patient outcomes.
Ana Leda F. Longhini   +15 more
doaj   +2 more sources

Chromosomes of Pseudapanteles dignus (Muesebeck, 1938) and a review of known karyotypes of the subfamily Microgastrinae (Hymenoptera, Braconidae) [PDF]

open access: yesComparative Cytogenetics
The karyotype of Pseudapanteles dignus (Muesebeck, 1938), an important parasitoid of a serious tomato pest Phthorimaea (= Tuta) absoluta Meyrick, 1917 (Lepidoptera, Gelechiidae), in the Neotropics and adjacent regions, was studied for the first time ...
Vladimir E. Gokhman   +3 more
doaj   +4 more sources

Chromosome number, genome size and heterochromatin evolution in diploid species of Ipomoea and related genera (Convolvulaceae: Convolvuloideae) [PDF]

open access: yesActa Botânica Brasílica, 2023
Convolvuloideae is a group of plants formed by genera with n = 15 and high stability of chromosome numbers. Despite being stable, polyploidy is the main mechanism of karyotype evolution for the group and seems to be related to speciation events.
Charlys Seixas Maia Dornelas   +4 more
doaj   +1 more source

Chromosomal diversity in three species of electric fish (Apteronotidae, Gymnotiformes) from the Amazon Basin [PDF]

open access: yesGenetics and Molecular Biology, 2014
Cytogenetic studies were carried out on samples of Parapteronotus hasemani, Sternarchogiton preto and Sternarchorhamphus muelleri (Apteronotidae, Gymnotiformes) from the Amazon basin. The first two species exhibited both a 2n = 52 karyotype, but differed
Fernando Henrique Ramos Silva   +5 more
doaj   +3 more sources

Spectral imaging and nucleic acid mimics fluorescence in situ hybridization (SI-NAM-FISH) for multiplex detection of clinical pathogens

open access: yesFrontiers in Microbiology, 2022
The application of nucleic acid mimics (NAMs), such as locked nucleic acid (LNA) and 2′-O-methyl-RNA (2’OMe), has improved the performance of fluorescence in situ hybridization (FISH) methods for the detection/location of clinical pathogens since they ...
Andreia S. Azevedo   +16 more
doaj   +1 more source

Progress and challenges in the use of fluorescence‐based flow cytometric assays for anti‐malarial drug susceptibility tests

open access: yesMalaria Journal, 2021
Drug-resistant Plasmodium is a frequent global threat in malaria eradication programmes, highlighting the need for new anti-malarial drugs and efficient detection of treatment failure.
Kasem Kulkeaw
doaj   +1 more source

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