Combination of small molecule microarray and confocal microscopy techniques for live cell staining fluorescent dye discovery [PDF]
Discovering new fluorochromes is significantly advanced by high-throughput screening (HTS) methods. In the present study a combination of small molecule microarray (SMM) prescreening and confocal laser scanning microscopy (CLSM) was developed in order to
Attila Bokros+10 more
core +2 more sources
A Technical Note on Quantum Dots for Multi-Color Fluorescence in situ Hybridization [PDF]
Quantum dots (Qdots) are semiconductor nanocrystals, which are photo-stable, show bright fluorescence with narrow, symmetric emission spectra and are available in multiple resolvable colors.
Cremer, M.+4 more
core +1 more source
Towards many colors in FISH on 3D-preserved interphase nuclei [PDF]
The article reviews the existing methods of multicolor FISH on nuclear targets, first of all, interphase chromosomes. FISH proper and image acquisition are considered as two related components of a single process.
Albiez, H.+9 more
core +1 more source
Seven-fluorochrome mouse M-FISH for high-resolution analysis of interchromosomal rearrangements [PDF]
The mouse has evolved to be the primary mammalian genetic model organism. Important applications include the modeling of human cancer and cloning experiments. In both settings, a detailed analysis of the mouse genome is essential.
Geigl, J.+3 more
core +1 more source
Advanced Fluorescence Microscopy Techniques-FRAP, FLIP, FLAP, FRET and FLIM [PDF]
Fluorescence microscopy provides an efficient and unique approach to study fixed and living cells because of its versatility, specificity, and high sensitivity.
Abbe+295 more
core +2 more sources
Multicolour interphase cytogenetics: 24 chromosome probes, 6 colours, 4 layers [PDF]
From the late 1980s onwards, the use of DNA probes to visualise sequences on individual chromosomes (fluorescent in-situ hybridisation - FISH) revolutionised the study of cytogenetics.
A.R. Thornhill+61 more
core +1 more source
Development of a Biological Dosimeter for Translocation Scoring Based on Two-Color Fluorescence in Situ Hybridization of Chromosome Subsets [PDF]
Recently fluorescence in situ hybridization protocols have been developed which allow the paining of individual chromosomes using DNA-libraries from sorted human chromosomes.
Cremer, Thomas, Popp, Susanne
core +1 more source
Multicolor fluorescence in situ hybridization on metaphase chromosomes and interphase Halo-preparations using cosmid and YAC clones for the simultaneous high resolution mapping of deletions in the dystrophin gene [PDF]
We report on multicolor fluorescence in situ hybridization protocols for the simultaneous visualization of deletion-prone regions for carrier detection of Duchenne/ Becker (DMD/BMD) muscular dystrophy.
Blonden, Lau+6 more
core +1 more source
A simple and sensitive flow cytometric assay for the determination of the cytotoxic activity of human natural killer cells [PDF]
A new, simple and sensitive flow cytometric assay for the determination of the cytotoxic activity of human natural killer cells is described. The assay is based on the use of two fluorochromes. The target cell population is stained with one fluorochrome (
Garritsen, Henk S.P.+4 more
core +3 more sources
Characterization of a new class of blue-fluorescent lipid droplet markers for live-cell imaging in plants. [PDF]
KEY MESSAGE: The present work demonstrates the use and advantages of novel, live cell permeable, lipid droplet localizing, non toxic, blue fluorochromes for use in live plant cells. Lipid droplets (LDs) are ubiquitous components of both animal and plant
Ayaydin, Ferhan+2 more
core +1 more source