Stimulated emission depletion microscopy with a single depletion laser using five fluorochromes and fluorescence lifetime phasor separation [PDF]
Stimulated emission depletion (STED) microscopy achieves super-resolution by exciting a diffraction-limited volume and then suppressing fluorescence in its outer parts by depletion.
Mariano Gonzalez Pisfil +5 more
doaj +2 more sources
Evaluation of three fluorochromes as in situ growth markers in Manila clam Ruditapes philippinarum
Marking technology is essential to evaluate the growth performance of shellfish species in aquaculture. Some fluorochromes have been widely used in shellfish growth studies, but their marking quality remains unresolved. In order to establish an effective
Lei Wu +7 more
doaj +2 more sources
Near-infrared fluorochromes (NIRF) are useful compounds for diverse biotechnology applications and for in vivo biomedical imaging. Such NIRF must have high quantum yield, be biocompatible, and be conjugatable to a wide variety of proteins, peptides, and ...
Yuhui Lin +2 more
doaj +2 more sources
Flow cytometric discrimination of seven lineage markers by using two fluorochromes. [PDF]
Flow cytometry is the primary immunological technique used to analyze multiple parameters on complex cell populations. We present a staining method that identifies major human mononuclear lymphoid and myeloid populations (CD4+ and CD8+ T cells, γδ T ...
Francesco Boin +6 more
doaj +3 more sources
DIRECT FLUOROCHROMING OF RICKETTSIAE [PDF]
Several fluorochromes were used to stains directly R. mooseri in smears and tissues. The effects of fixatives, hydrogen ion concentration, time in stain and concentration of stain were studied also. Interference by background fluoresence was minimized by a preliminary staining of fixed preparations with an alcian blue, periodic acid-Schiff sequence ...
D, ANDERSON, D, GREIFF
openaire +2 more sources
Cellular Dynamics and Dentine Bridge Formation Following Direct Pulp Capping With MTA and Biodentine in a Murine Model. [PDF]
ABSTRACT Aim Formation of a mineralized dentine bridge is the hallmark of successful direct pulp capping (DPC). Mineral trioxide aggregate (MTA) and Biodentine are widely used capping materials, but in vivo evidence comparing their regenerative potential remains inconclusive. This study used a controlled murine model to compare dentine bridge formation
Sajdeya A +4 more
europepmc +2 more sources
The chromosome number and heterochromatin distribution pattern suggest differentiation among species of the Stylosanthes scabra-seabrana complex [PDF]
The genus Stylosanthes has economic importance in semi-arid regions and requires studies that reveal complex relationships between species involving different ploidies.
IRLANE CRISTINE S.A. LIRA +4 more
doaj +1 more source
A Strategy for Multiple Immunophenotyping by Image Cytometry: Model Studies Using Latex Microbeads Labeled with Seven Streptavidin-Bound Fluorochromes [PDF]
peer reviewedMultiple immunophenotyping is aimed at identifying several cell populations in a single labeling procedure by their ability to bind combinations of specific labeled antibodies. The present work demonstrates the simultaneous discrimination by
Gothot, André +2 more
core +1 more source
The stingless bees Melipona rufiventris and M. mondury were analyzed cytogenetically by conventional staining with Giemsa, C-banding and sequential staining with the fluorochromes CMA3/DA/DAPI.
Denilce Meneses Lopes +3 more
doaj +1 more source
Stepping up ELISpot: Multi-Level Analysis in FluoroSpot Assays
ELISpot is one of the most commonly used immune monitoring assays, which allows the functional assessment of the immune system at the single cell level.
Sylvia Janetzki +2 more
doaj +1 more source

