Results 91 to 100 of about 17,088 (300)

Comparative cytogenetic analysis between Lonchorhina aurita and Trachops cirrhosus (Chiroptera, Phyllostomidae)

Genetics and Molecular Biology, 2009
Phyllostomidae comprises the most diverse family of neotropical bats, its wide range of morphological features leading to uncertainty regarding phylogenetic relationships.
Helen Maria Duarte do Rêgo Barros, Cibele Gomes de Sotero-Caio, Neide Santos, Maria José de Souza   +3 more
doaj  

Protocol for high-sensitivity/long linear-range spectrofluorimetric DNA quantification using ethidium bromide

BioTechniques, 2007
Ethidium bromide (EtBr) is the most widely used fluorescent dye in nucleic acid gel electrophoresis since decades. However, it has been essentially forgotten in DNA quantification by spectrofluorimetry.
Veronica Bonasera, Saverio Alberti, Andrea Sacchetti   +2 more
doaj   +1 more source

Flow cytometric fluorescence lifetime analysis of DNA binding fluorochromes [PDF]

, 2002
Most flow cytometry (FCM) applications monitor fluorescence intensity to quantitate the various cellular parameters; however, the fluorescence emission also contains information relative to the fluorescence lifetime. Recent developments in FCM (Pinsky et
Steinkamp, J. A., Cui, H. H. (H. Helen), Crissman, Harry A.   +2 more
core  

DNA Binding Fluorochromes as Probes for Histone HI-Chromatin Interactions In Situ

, 1997
We have investigated using the DNA binding fluorochromes 7-aminoactinomycin (7-AAMD) and 4′,6-diamidino-2-phenylindole (DAPI) as cytochemical probes for linker histone (H1)–chromatin interactions in situ.
Rundquist, Ingemar,, Loborg, Helena,
core   +1 more source

Restores Aβ Clearance by Overcoming PCSK9–LRP1 Dysregulation and TRIB3‐Mediated Autophagy Blockade in Alzheimer's Disease

Advanced Science, EarlyView.
PCSK9 upregulation in cerebrovascular endothelial cells and microglia destabilizes the Aβ transporter LRP1, while TRIB3‐associated autophagy blockade further limits intracellular Aβ degradation. A RAP‐modified tea‐polyphenol nanoparticle co‐delivers siPCSK9 and siTRIB3 to restore LRP1‐mediated Aβ efflux and autophagy‐dependent degradation, reducing Aβ ...
Jie Miao, Jing Wang, Yankun Li, Haipei Zhang, Yanli Zhang, Qingnian Li, Bo Xiao, Wenhu Zhou, Junhong Guo   +8 more
wiley   +1 more source

Flow Cytometry as a Tool for Quality Control of Fluorescent Conjugates Used in Immunoassays.

PLoS ONE, 2016
The use of antibodies in immunodiagnostic kits generally implies the conjugation of these proteins with other molecules such as chromophores or fluorochromes.
Marta de Almeida Santiago, Bruna de Paula Fonseca E Fonseca, Christiane de Fátima da Silva Marques, Edimilson Domingos da Silva, Alvaro Luiz Bertho, Ana Cristina Martins de Almeida Nogueira   +5 more
doaj   +1 more source

Dyes & fluorochromes as imaging agents in biology and medicine - new opportunities for dyestuff and textile chemists

, 2012
Dyes and fluorochromes are used in biology and medicine to generate informative microscopic and macroscopic images. This means that the presence, location, biological structure, chemical content and physiological function of cells, tissues and whole ...
Horobin, Richard W.
core   +1 more source

Remote Ischemic Post‐Conditioning Preserves the Neurovascular Unit by Brain‐Derived Gas6 That Drives Monocyte‐Derived Macrophage Reprogramming via Axl and Vimentin after SAH

Advanced Science, EarlyView.
Subarachnoid hemorrhage (SAH) triggers monocyte‐derived macrophage (MDM) infiltration and M1‐like polarization via Vimentin upregulation, leading to neurovascular unit disruption and cognitive dysfunction. Remote ischemic postconditioning (RIPostC) upregulates neuronal Gas6, which activates Axl on MDMs to downregulate Vimentin and drive reparative M2 ...
Yajun Zhu, Zichao Huang, Xi Rong, Xingwei Lei, Fuming Liang, Xiaoguo Li, Jiru Zhou, Yu Wang, Yifan Huang, Shuang Tang, Jianjun Zhong, Jin Yan, Hongji Deng, Chun Zeng, Zongduo Guo   +14 more
wiley   +1 more source

Engineered Peptide Self‐Assembled Nanofibers for Enhanced STING Activation: A Promising Nanodelivery Platform for Immunotherapy of Pediatric Solid Tumors

Advanced Science, EarlyView.
An engineered peptide self‐assembled nanofiber platform, designed to replicate STING–cGAMP binding motifs, facilitates pH‐responsive cytosolic delivery of cGAMP, enhances the endoplasmic reticulum with high efficiency, and robustly activates the STING pathway.
Yubin He, Xiao Lu, Chang qing Yang, Ran Zhang, Bo Wan, Xin Ni, Dong Mei   +6 more
wiley   +1 more source

DNA stainability with base-specific fluorochromes: dependence on the DNA topology in situ.

, 1994
The influence of DNA topology on stainability with the externally binding fluorochromes Hoechst 33258 (HO) and mithramycin (MI) was investigated in HeLa nuclei in comparison with the intercalating dye propidium iodide (PI).
Bottiroli G, Prosperi E, Giangarè MC
core