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The consequences of mismatched buffers in spectral cell sorting
Dapaah RAS+9 more
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Detecting Fluorochrome-Labeled Cells
Cold Spring Harbor Protocols, 2021This protocol describes cell staining using fluorochrome-labeled antibodies. The resolution of subcellular structures using fluorochrome-labeled antibodies exceeds that of the transmitted light microscope because of the visualization of an expanding cone of emitted light from the excited fluorochrome in the specimen.
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Conjugation of Fluorochromes to Antibodies
1994Immunolocalization of antigen via fluorescence requires that fluorochromes be linked either to the primary antibody (direct method) or to a second antibody (indirect method) to provide a fluorescent signal to mark the site of antibody-antigen binding. Of these two methods, the indirect technique is generally more useful and practical. Fluorochromes can
Su-Yau, Mao, J Michael, Mullins
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Fluorochrome Staining of Multilamellar Liposomes
Stain Technology, 1978Multilamellar liposomes can be stained with such fluorochromes as acridine orange, eosin Y, neutral red, and thiazine red. The liposomes are brought into a 1% solution of the fluorochrome; 5-10 minutes later they are centrifuged and washed by resuspending in water or phosphate buffered saline three times.
N. van Rooijen, R. van Nieuwmegen
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Fluorochromes for DNA Staining and Quantitation
2017In these last few decades the great explosion of the molecular approaches has casted a little shadow on the DNA quantitative analysis. Nevertheless DNA cytochemistry represented a long piece of history in cell biology since the advent of the Feulgen reaction.
Mazzini G, Danova M
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Fluorochrome in Monitoring Indoor Bioaerosols
Aerosol Science and Technology, 2006In this study total concentration, viability, culturability of indoor and outdoor bioaerosols in ten residential environments were studied by using a cultivating method and an epifluorescence microscopy method with fluorochrome (EFM/FL) using four different dyes (AO, DAPI, PI, and YOPRO-1). Results from the EFM/FL were then compared with those from the
LI, CHIH-SHAN, HUANG, TZU-YI
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Bioaerosol characterization by flow cytometry with fluorochrome
Journal of Environmental Monitoring, 2005Traditional culture and microscopy methods for evaluation of bioaerosols are slow, tedious, and rather imprecise. In this study, the application of flow cytometry that was combined with a fluorescent technique (FCM/FL) was evaluated as a technique to quickly and accurately determine and quantify the total concentration and viability of bioaerosols. The
Chen, Pei-Shih, Li, Chih-Shan
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Heterochromatin recognition with fluorochromes
Chromosoma, 1970Some types of acridine derivative and especially Quinacrine dihydrochloride and its mustard may be successfully used as chromosome marker and to investigate the chemical differentiation of euchromatin and heterochromatin. — There are at least four main types of heterochromatin, showing all possible combinations of positive and negative cold effect ...
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Physiological assessment of bacteria using fluorochromes
Journal of Microbiological Methods, 1995This minireview focuses on the application of fluorogenic compounds in the detection of bacteria with particular emphasis on the assessment of physiological activity using epifluorescence microscopy. Microbiological applications of several related methods will also be reviewed.
Barry H. Pyle+3 more
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Marking Fish with Fluorochrome Dyes
Reviews in Fisheries Science & Aquaculture, 2019AbstractMarking fish with fluorochrome dyes has the potential to produce detectable marks in otoliths, scales, fin rays, fin spines, and other bony structures based on differential staining of chem...
Hongjian Lü+4 more
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