Results 11 to 20 of about 26,681 (143)

Conjugation of Fluorochromes to Monoclonal Antibodies. [PDF]

Curr Protoc, 2023
AbstractDetection of cell surface molecules labeled by monoclonal or polyclonal antibodies conjugated to a fluorochrome is the most widely used application of flow cytometry. Here, we present protocols for tagging monoclonal antibodies with fluorescein, biotin, Texas Red, and phycobiliproteins.
Lantz L, Holmes K, Douagi I.
europepmc   +5 more sources

DIRECT FLUOROCHROMING OF RICKETTSIAE [PDF]

Journal of Histochemistry & Cytochemistry, 1964
Several fluorochromes were used to stains directly R. mooseri in smears and tissues. The effects of fixatives, hydrogen ion concentration, time in stain and concentration of stain were studied also. Interference by background fluoresence was minimized by a preliminary staining of fixed preparations with an alcian blue, periodic acid-Schiff sequence ...
Doris Anderson, Donald Greiff
openaire   +3 more sources

C-banding, fluorescent staining and NOR location in holokinetic chromosomes of bugs of the Neotropical genus Antiteuchus (Heteroptera: Pentatomidae: Discocephalinae) [PDF]

, 2006
Different cytogenetic techniques including C-banding, base-specific fluorochromes and silver nitrate staining were used to compare the karyotypes of three species of bugs, representatives of the Neotropical genus Antiteuchus, namely A.
de Souza, Maria José, Lanzone, Cecilia
core   +1 more source

Approaches to overcome flow cytometry limitations in the analysis of cells from veterinary relevant species [PDF]

, 2020
BACKGROUND: Flow cytometry is a powerful tool for the multiparameter analysis of leukocyte subsets on the single cell level. Recent advances have greatly increased the number of fluorochrome-labeled antibodies in flow cytometry.
Debes, Gudrun F., Hunka, Julia, Riley, John T   +2 more
core   +1 more source

Out-of-equilibrium collective oscillation as phonon condensation in a model protein [PDF]

, 2018
In the first part of the present paper (theoretical), the activation of out-of-equilibrium collective oscillations of a macromolecule is described as a classical phonon condensation phenomenon. If a macromolecule is modeled as an open system, that is, it
Donato, Irene, Giliberti, Valeria, Gori, Matteo, Lechelon, Mathias, Meriguet, Yoann, Nardecchia, Ilaria, Nouvel, Philippe, Ortolani, Michele, Pettini, Marco, Preto, Jordane, Sturgis, James, Torres, Jeremie, Varani, Luca   +12 more
core   +3 more sources

Staining autoradiographs with the fluorochrome “33258” [PDF]

The Science of Nature, 1976
feuchtigkeitsempfindlich. Von der IridiumVerbindung konnten durch 800stfindiges Tempern bei 1000 ~ Einkristalle erhalten werden. In Tabelle 1 sind neben den pyknometrisch besfimmten Dichten die r6ntgenographischen Daten angegeben, die beim Lil,e3IrB2 aus Pr~izessionsaufnahmen, bei den beiden anderen Verbindungen aus Guinier-Aufnahmen ermittelt wurden ...
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Karyotypic variation in the Andean rodent Phyllotis xanthopygus (Waterhouse, 1837) (Rodentia, Cricetidae, Sigmodontinae) [PDF]

, 2014
Phyllotis xanthopygus (Waterhouse, 1837) is an Andean rodent endemic to South America. Despite its wide geographical distribution in Argentina, few individuals have been studied on the cytogenetic level and only through conventional staining.
Cuello, Pablo Andrés, Labaroni, Carolina Alicia, Lanzone, Cecilia, Malleret, Matías Maximiliano, Marti, Dardo Andrea, Novillo, Agustina, Ojeda, Agustina Alejandra, Ojeda, Agustina Alejandra, Rodriguez, Daniela   +8 more
core   +4 more sources

Selecting fluorochrome conjugates for maximum sensitivity [PDF]

Cytometry Part A, 2004
Most users of flow cytometry are aware that, to maximize sensitivity for an antibody whose staining is dim, a bright fluorochrome such as phycoerythrin (PE) should be selected for that conjugate. What is often less well appreciated is the role that spectral overlap plays in determining the sensitivity of a given fluorescence channel.
Joe Trotter, Laurel Nomura, Holden T. Maecker, Tom Frey   +3 more
openaire   +3 more sources

Detection of complete and partial chromosome gains and losses by comparative genomic in situ hybridization [PDF]

, 1993
Comparative genomic in situ hybridization (CGH) provides a new possibility for searching genomes for imbalanced genetic material. Labeled genomic test DNA, prepared from clinical or tumor specimens, is mixed with differently labeled control DNA prepared ...
Cremer, Thomas, Döhner, Hartmut, Joos, Stefan, Kovacs, Gyula, Lichter, Peter, Manoir, Stanislas du, Popp, Susanne, Robert-Nicoud, Michel, Schröck, Evelin, Speicher, Michael R.   +9 more
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A strategy for the characterization of minute chromosome rearrangements using multiple color fluorescence in situ hybridization with chromosome-specific DNA libraries and YAC clones [PDF]

, 1993
The identification of marker chromosomes in clinical and tumor cytogenetics by chromosome banding analysis can create problems. In this study, we present a strategy to define minute chromosomal rearrangements by multicolor fluorescence in situ ...
A Kallioniemi, Anna Jauch, B Dutrillaux, C Lengauer, C Lengauer, CC Collins, Christoph Lengauer, D Pinkel, Detlev Schindler, DH Ledbetter, DH Ledbetter, GD Johnson, H Telenius, Harold C. Riethman, Helen Donis-Keller, J Grouchy de, J Sambrook, J Wiegant, JG Dauwerse, JS Heslop-Harrison, JW IJdo, Michael R. Speicher, P Lichter, P Lichter, PM Nederlof, PM Nederlof, S Manoir du, Susanne Popp, T Ried, T Ried, T Ried, Thomas Cremer   +31 more
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