Results 241 to 250 of about 17,088 (300)

Use of fluorochromes in the study of fungal cytology and differentiation

Experimental Mycology, 1989
Raymond St. Leger, Tariq M Butt, Harvey C Hoch   +2 more
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Conjugation of Fluorochromes to Monoclonal Antibodies

Current Protocols, 1997
AbstractDetection of cell surface molecules labeled by monoclonal or polyclonal antibodies conjugated to a fluorochrome is the most widely used application of flow cytometry. Here, we present protocols for tagging monoclonal antibodies with fluorescein, biotin, Texas Red, and phycobiliproteins.
K L, Holmes, L M, Lantz, W, Russ
openaire   +3 more sources

Physiological assessment of bacteria using fluorochromes

Journal of Microbiological Methods, 1995
This minireview focuses on the application of fluorogenic compounds in the detection of bacteria with particular emphasis on the assessment of physiological activity using epifluorescence microscopy. Microbiological applications of several related methods will also be reviewed.
Philip Stewart, Gordon A McFeters, B H Pyle   +2 more
exaly   +3 more sources

Detecting Fluorochrome-Labeled Cells

Cold Spring Harbor Protocols, 2021
This protocol describes cell staining using fluorochrome-labeled antibodies. The resolution of subcellular structures using fluorochrome-labeled antibodies exceeds that of the transmitted light microscope because of the visualization of an expanding cone of emitted light from the excited fluorochrome in the specimen.
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Conjugation of Fluorochromes to Antibodies

2009
Immunolocalization of antigen via fluorescence requires that fluorochromes be linked either to the primary antibody (direct method) or to a second antibody (indirect method) to provide a fluorescent signal to mark the site of antibody-antigen binding. Of these two methods, the indirect technique is generally more useful and practical. Fluorochromes can
Su-Yau, Mao, J Michael, Mullins
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Fluorochromes for DNA Staining and Quantitation

2017
In these last few decades the great explosion of the molecular approaches has casted a little shadow on the DNA quantitative analysis. Nevertheless DNA cytochemistry represented a long piece of history in cell biology since the advent of the Feulgen reaction.
Mazzini G, Danova M
openaire   +2 more sources

Fluorochrome Staining of Multilamellar Liposomes

Stain Technology, 1978
Multilamellar liposomes can be stained with such fluorochromes as acridine orange, eosin Y, neutral red, and thiazine red. The liposomes are brought into a 1% solution of the fluorochrome; 5-10 minutes later they are centrifuged and washed by resuspending in water or phosphate buffered saline three times.
N, van Rooijen, R, van Nieuwmegen
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Fluorochromes: Properties and Characteristics

2009
Immunofluorescence microscopy provides a sensitive means by which antigens can be localized within tissues or individual cells. For the most effective use of this technique the researcher can draw upon basic information on factors that affect the brightness of the fluorescence image, and how well that image can be distinguished from background ...
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Selective `in synthesis' labelling of peptides by fluorochromes

Biochimica et Biophysica Acta (BBA) - General Subjects, 1997
A new method is described for producing fluorescently-tagged peptides containing specific internal derivatives of lysyl residues. The technique employs the base-labile Boc-Lys(Fmoc)-COOH derivative with base-catalyzed removal of the Fmoc protecting group during peptide synthesis and subsequent fluorescent derivatization of the deprotected epsilon-amino
A, Chersi, F, di Modugno, L, Rosanò
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Bioaerosol characterization by flow cytometry with fluorochrome

Journal of Environmental Monitoring, 2005
Traditional culture and microscopy methods for evaluation of bioaerosols are slow, tedious, and rather imprecise. In this study, the application of flow cytometry that was combined with a fluorescent technique (FCM/FL) was evaluated as a technique to quickly and accurately determine and quantify the total concentration and viability of bioaerosols. The
Chen, Pei-Shih, Li, Chih-Shan
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