Results 41 to 50 of about 117,479 (219)

Single-Labeled Oligonucleotides Showing Fluorescence Changes upon Hybridization with Target Nucleic Acids

Molecules, 2018
Sequence-specific detection of nucleic acids has been intensively studied in the field of molecular diagnostics. In particular, the detection and analysis of single-nucleotide polymorphisms (SNPs) is crucial for the identification of disease-causing ...
Gil Tae Hwang
doaj   +1 more source

A Novel Way to Monitor Urine Concentration: Fluorescent Concentration Matrices [PDF]

Journal of Clinical and Diagnostic Research, 2015
Background: The amount of water found in urine is important diagnostic information; nevertheless it is not yet directly determined. Indirectly, the water content in urine is expressed by its density (specific gravity).
Katarina Dubayova, Iveta Luckova, Jan Sabo, Anton Karabinos   +3 more
doaj   +1 more source

USP29‐regulated noncanonical stabilization of the hypoxia‐inducible factor‐α in aggressive prostate cancer

Molecular Oncology, EarlyView.
We identify USP29 as the only DUB mirroring CA9 expression, a marker of hypoxia and HIF pathway activation associated with PCA aggressiveness. USP29 stabilizes HIF‐1α and HIF‐2α via a noncanonical mechanism that is independent of PHD/pVHL activity yet relies on proteasomal regulation, establishing USP29 as a previously unrecognized regulator of hypoxic
Amelie S Schober, Leire Moreno‐Cugnon, Laura Martínez‐Pérez, Amaia Ercilla, Amaia Zabala‐Letona, Adrián Vicente‐Barrueco, Maider Fagoaga‐Eugui, Saioa Garcia‐Longarte, Blanca Calle‐Ciborro, Encarnación Pérez‐Andrés, Onintza Carlevaris, Sara Pozo, Isabel Mendizabal, Ugo Mayor, Arkaitz Carracedo, Violaine Sée, Edurne Berra   +16 more
wiley   +1 more source

Design of PEGylated Three Ligands Silica Nanoparticles for Multi-Receptor Targeting

Nanomaterials, 2021
The synthesis of silica nanoparticles (SiNPs) decorated on their surface with a range of various elements (e.g., ligands, drugs, fluorophores, vectors, etc.) in a controlled ratio remains a big challenge.
Manon Maurel, Titouan Montheil, Julie Martin, Line Chaar, Veronica Guzman-Gonzalez, Morgane Couvet, Thibault Jacquet, Tao Jia, Beatrice Eymin, Karine Parra, Pascal Dumy, Jean Martinez, Florence Ruggiero, Elisabeth Vaganay, Ahmad Mehdi, Jean-Luc Coll, Gilles Subra   +16 more
doaj   +1 more source

Pharmacological chromatin remodeling enhances response to estrogen therapy in ER+ breast cancer

Molecular Oncology, EarlyView.
Estrogen therapy elicits clinical benefit in ~ 30% of patients with endocrine‐resistant estrogen receptor (ER)‐positive breast cancer. Based on findings that ER transcriptional activation underlies response to estrogen therapy, we tested the effects of epigenetic dysregulation via pharmacological inhibition of histone deacetylases (HDACi).
Anneka L. Johnson Thomas, Barbara Karakyriakou, Patricia C. Muskus, Alyssa M. Roberts, Justin Rendleman, Alexa Warren, Hanxu Lu, Kimberly A. Birmingham, Malone K. Friedman, Abigail E. Goen, Huijuan Yang, Owen M. Wilkins, Fred W. Kolling, Eugene Demidenko, Julie M. Jorns, Brock C. Christensen, Todd W. Miller   +16 more
wiley   +1 more source

Selective Activity-Based Probes Targeting Fibroblast Activation Protein (FAP)

Proceedings, 2019
Fibroblast activation protein (FAP) is a type II transmembrane serine protease that belongs tothe dipeptidyl peptidase (DPP) family. [...]
Johannes Vrijdag, Anvesh Jallapally, An De Decker, Koen Janssen, Filipe Elvas, Steven Staelens, Ingrid De Meester, Koen Augustyns, Anne-Marie Lambeir, Pieter Van der Veken   +9 more
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Optimizing photoactivation of PA‐mCherry for optical pooled CRISPR screens

FEBS Open Bio, EarlyView.
Photoactivatable PA‐mCherry finds widespread use to optically tag individual cells. However, confocal 405 nm UV laser‐scanning (normal scan) is much less efficient than widefield UV illumination, limiting the use of PA‐mCherry on confocal instruments. We remedy this limitation by reporting that rapid and repeated confocal scanning with a low‐intensity,
Sravasti Mukherjee, Giulia Zanetti, Bram van den Broek, Kees Jalink   +3 more
wiley   +1 more source

Visualizing nanostructures in supramolecular hydrogels: a correlative study using confocal and cryogenic scanning electron microscopy

Beilstein Journal of Nanotechnology
Solvated supramolecular hydrogels present unique challenges in nanoscale morphological characterization because of their fragile fibrous nature and low concentration of the solid component.
Shaun M. Smith, Ferdinando Malagreca, Jacqueline Hicks, Giuseppe Mantovani, David B. Amabilino, Christopher Parmenter, Lluïsa Pérez-García   +6 more
doaj   +1 more source

Chromatic Bacteria – A Broad Host-Range Plasmid and Chromosomal Insertion Toolbox for Fluorescent Protein Expression in Bacteria

Frontiers in Microbiology, 2018
Differential fluorescent labeling of bacteria has become instrumental for many aspects of microbiological research, such as the study of biofilm formation, bacterial individuality, evolution, and bacterial behavior in complex environments.
Rudolf O. Schlechter, Rudolf O. Schlechter, Hyunwoo Jun, Michał Bernach, Michał Bernach, Simisola Oso, Erica Boyd, Dian A. Muñoz-Lintz, Renwick C. J. Dobson, Renwick C. J. Dobson, Renwick C. J. Dobson, Daniela M. Remus, Daniela M. Remus, Daniela M. Remus, Mitja N. P. Remus-Emsermann, Mitja N. P. Remus-Emsermann   +15 more
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To What Extent Do Fluorophores Bias the Biological Activity of Peptides? A Practical Approach Using Membrane-Active Peptides as Models

Frontiers in Bioengineering and Biotechnology, 2020
The characterization of biologically active peptides relies heavily on the study of their efficacy, toxicity, mechanism of action, cellular uptake, or intracellular location, using both in vitro and in vivo studies. These studies frequently depend on the
Marco Cavaco, Marco Cavaco, Clara Pérez-Peinado, Javier Valle, Rúben D. M. Silva, João D. G. Correia, David Andreu, Miguel A. R. B. Castanho, Vera Neves   +8 more
doaj   +1 more source