Results 51 to 60 of about 132,621 (262)

Genotype-independent transmission of transgenic fluorophore protein by boar spermatozoa. [PDF]

, 2011
Recently, we generated transposon-transgenic boars (Sus scrofa), which carry three monomeric copies of a fluorophore marker gene. Amazingly, a ubiquitous fluorophore expression in somatic, as well as in germ cells was found.
Ehling, C., Ivics Zoltán, Stephanie Holler, Holler Stephanie, Wilfried A Kues, Christina Struckmann, Garrels Wiebke, Kues, Wilfried August, Christine Ehling, Herrmann, D., Struckmann Christina, Struckmann, C., Ivics, Z., Brigitte Barg-Kues, Nowak-Imiałek, Monika Anna, Ivics, Zoltán, Holler, S., Herrmann, Doris, Heiner Niemann, Detlef Rath, Barg-Kues Brigitte, Holler, Stephanie, Wilfried A. Kues, Barg-Kues, Brigitte, Niemann, Heiner, Kues, W.A., Sabine Klein, Klein Sabine, Nowak-Imialek Monika, Barg-Kues, B., Wiebke Garrels, Taylor Ulrike, Ulrike Taylor, Herrmann Doris, Ehling Christine, Garrels, W., Niemann, H., Rath, Detlef, Zoltán Ivics, Rath Detlef, Taylor, Ulrike, Monika Nowak-Imialek, Nowak-Imialek, M., Klein, S., Taylor, U., Ehling, Christine, Klein, Sabine, Kues Wilfried A., Niemann Heiner, Doris Herrmann, Garrels, Wiebke, Rath, D., Struckmann, Christina   +52 more
core   +1 more source

Photoactivatable Fluorophores [PDF]

ISRN Physical Chemistry, 2012
Photoactivatable fluorophores switch from a nonemissive to an emissive state upon illumination at an activating wavelength and then emit after irradiation at an exciting wavelength. The interplay of such activation and excitation events can be exploited to switch fluorescence on in a defined region of space at a given interval of time.
openaire   +1 more source

Tracking Spatiotemporal Extracellular Matrix Evolution and Tissue Fusion in 3D Microtissues via Click Chemistry‐Based Metabolic Labelling

Advanced Healthcare Materials, EarlyView.
Metabolic labeling of nascent proteins in 3D microtissue spheroids provides a powerful analytical approach for large‐scale tissue engineering. Incorporation of non‐canonical amino acids with fluorescent tagging enables spatiotemporal investigation of extracellular matrix deposition and its evolution during multicellular tissue development and fusion ...
Theresa Koenig, F. Max Yavitt, Laura Veenendaal, Tim B. F. Woodfield   +3 more
wiley   +1 more source

Fluorophore-Tagged Poly-Lysine RAFT Agents: Controlled Synthesis of Trackable Cell-Penetrating Peptide–Polymers

The conjugation of a fluorophore and a variety of cell-penetrating peptides onto a RAFT agent allowed for the synthesis of polymers of defined sizes with quantifiable cell-uptake.
Maxime Klausen (15337516), Annamaria Lilienkampf (1547581), Mark Bradley (39976), Paige A. Shaw (16963983)   +3 more
core   +1 more source

Alkyl-Cobalamin: Fluorophore-Enhanced Photolysis

, 2019
Light-activatable drugs offer the advantage of exquisite spatio-temporal control in drug release, thereby reducing off-target effects in patients. However, they are limited by lights ability to penetrate tissue and cause drug release.
Hartman, Timothy
core   +1 more source

Plasmonic Enhancement of Fluorescence and Protein Dynamics in Living Mammalian Cells

Advanced Materials, EarlyView.
This study demonstrates plasmonic enhancement of the function of fluorescent voltage sensing proteins (genetically encoded voltage indicators, (GEVIs), QuasAr6) in live mammalian cells. Coupling to plasmonic nanoparticles does not just increase fluorescence, but influences the protein photocycle, creating a hybrid sensor with its response speed to ...
Marco Locarno, Qiangrui Dong, Marco Post, Xin Meng, Cristiano Glessi, Nynke Marije Hettema, Nidas Brandsma, Sebbe Blokhuizen, Alejandro Castañeda Garcia, Srividya Ganapathy, Thieme Schmidt, Lars van Roemburg, Bing Xu, Chun‐Ting Cho, Liedewij Laan, Miao‐Ping Chien, Daan Brinks   +16 more
wiley   +1 more source

Bacteria‐Responsive Nanostructured Drug Delivery Systems for Targeted Antimicrobial Therapy

Advanced Materials, EarlyView.
Bacteria‐responsive nanocarriers are designed to release antimicrobials only in the presence of infection‐specific cues. This selective activation ensures drug release precisely at the site of infection, avoiding premature or indiscriminate release, and enhancing efficacy.
Guillermo Landa, Gracia Mendoza, Silvia Irusta, Manuel Arruebo   +3 more
wiley   +1 more source

An aryleneethynylene fluorophore for cell membrane staining

, 2012
The use of an amphiphilic aryleneethynylene fluorophore as a plasma membrane marker in fixed and living mammalian cells and liposome model systems is demonstrated. We show here that the optical properties of the novel dye are almost independent on pH, in
Farinola, Gianluca M., Affortunato, Francesco, Colella, Matilde, Farinola, Gianluca, Cardone, Antonio, Mallamaci, Rosanna, Martinelli, Carmela, Hofer, Aldebaran, Hofer, Aldebaran M., Busco, Giovanni, Lopez, Francesco   +10 more
core   +2 more sources

Fluorescence-Based Nitric Oxide Detection by Ruthenium Porphyrin Fluorophore Complexes

, 2016
The ruthenium(II) porphyrin fluorophore complexes [Ru(TPP)(CO)(Ds-R)] (TPP = tetraphenylporphinato dianion; Ds = dansyl; R = imidazole (im), 1, or thiomorpholine (tm), 2) were synthesized and investigated for their ability to detect nitric oxide (NO ...
Mi Hee Lim (1474606), Stephen J. Lippard (1233759)   +1 more
core   +3 more sources

Counterintuitive Fluorescence Blue Shift in Symmetry Breaking Dicationic Bis(indolium) with Two‐Photon Absorption Properties for NIR Living Cell Imaging

Advanced Materials, EarlyView.
Highly water‐soluble dicationic bis(indolium) dyes have been prepared, showing excellent two‐photon absorption and light emission. Their fluorescence behavior discloses an unusual increasing blue shift with increasing solvent polarity, which, in parallel, is beneficial for enhanced detection in biological media.
Carlos Benitez‐Martin, Jose M. Marin‐Beloqui, Juan T. López Navarrete, Juan Casado, Francisco Najera, Ezequiel Perez‐Inestrosa   +5 more
wiley   +1 more source