Results 1 to 10 of about 39,076 (213)

Heterologous expression of formate dehydrogenase enables photoformatotrophy in the emerging model microalga, Picochlorum renovo [PDF]

Frontiers in Bioengineering and Biotechnology, 2023
Rising global greenhouse gas emissions and the impacts of resultant climate change necessitate development and deployment of carbon capture and conversion technologies. Amongst the myriad of bio-based conversion approaches under evaluation, a formate bio-
Lukas R. Dahlin, Alex W. Meyers, Skylar W. Stefani, Ellsbeth G. Webb, Benton Wachter, Venkataramanan Subramanian, Michael T. Guarnieri, Michael T. Guarnieri, Michael T. Guarnieri   +8 more
doaj   +2 more sources

Fast growth and high-titer bioproduction from renewable formate via metal-dependent formate dehydrogenase in Escherichia coli [PDF]

Nature Communications
Microbial bioproduction using one-carbon (C1) feedstocks has the potential to decarbonize the manufacturing of materials, fuels, and chemicals. Formate is a promising C1 feedstock, and the realization of industrial, formatotrophic platform organisms is a
Aidan E. Cowan, Mason Hillers, Vittorio Rainaldi, Florent Collas, Hemant Choudhary, Basem S. Zakaria, Gregory G. Bieberach, David N. Carruthers, Maxwell Grabovac, Jennifer W. Gin, Bridgie Cawthon, Yan Chen, Emine Akyuz Turumtay, Edward E. K. Baidoo, Christopher J. Petzold, Adam M. Feist, Sara Tejedor-Sanz, Frank Kensy, Blake A. Simmons, Jay D. Keasling, Nico J. Claassens   +20 more
doaj   +2 more sources

Real flue gas CO2 hydrogenation to formate by an enzymatic reactor using O2- and CO-tolerant hydrogenase and formate dehydrogenase [PDF]

Frontiers in Bioengineering and Biotechnology, 2023
It is challenging to capture carbon dioxide (CO2), a major greenhouse gas in the atmosphere, due to its high chemical stability. One potential practical solution to eliminate CO2 is to convert CO2 into formate using hydrogen (H2) (CO2 hydrogenation ...
Jaehyun Cha, Jinhee Lee, Byoung Wook Jeon, Yong Hwan Kim, Inchan Kwon, Inchan Kwon   +5 more
doaj   +2 more sources

Bioelectrochemical conversion of CO2 to value added product formate using engineered Methylobacterium extorquens [PDF]

Scientific Reports, 2018
The conversion of carbon dioxide to formate is a fundamental step for building C1 chemical platforms. Methylobacterium extorquens AM1 was reported to show remarkable activity converting carbon dioxide into formate.
Jungho Jang, Byoung Wook Jeon, Yong Hwan Kim   +2 more
doaj   +2 more sources

Formate Dehydrogenase Mimics as Catalysts for Carbon Dioxide Reduction [PDF]

Molecules, 2022
Formate dehydrogenases (FDH) reversibly catalyze the interconversion of CO2 to formate. They belong to the family of molybdenum and tungsten-dependent oxidoreductases. For several decades, scientists have been synthesizing structural and functional model
Thibault Fogeron, Yun Li, Marc Fontecave
doaj   +2 more sources

Sorbitol Uptake and Oxygen Transfer Shape AOX1 Promoter Induction in Formate Dehydrogenase‐Deficient Komagataella phaffii [PDF]

Microbial Biotechnology
In Komagataella phaffii, the use of formate as an AOX1 promoter (PAOX1) inducer in combination with sorbitol, a non‐repressive carbon source, has emerged as a promising alternative to methanol‐based expression systems.
Cristina Bustos, Rocio Cozmar, Julio Berrios, Patrick Fickers   +3 more
doaj   +2 more sources

Formate from THF‐C1 metabolism induces the AOX1 promoter in formate dehydrogenase‐deficient Komagataella phaffii [PDF]

Microbial Biotechnology
In Komagataella phaffii (Pichia pastoris), formate is a recognized alternative inducer to methanol for expression systems based on the AOX1 promoter (pAOX1).
Cristina Bustos, Julio Berrios, Patrick Fickers   +2 more
doaj   +2 more sources

Functional expression of a Mo-dependent formate dehydrogenase in Escherichia coli under aerobic conditions. [PDF]

PLoS ONE
BackgroundOxygen tolerant complex metal-dependent formate dehydrogenases hold potential for biotechnological applications.Principal findingsIn this work, we report the functional expression of the complex, molybdenum-dependent soluble formate ...
Marion Schulz, Anne Berger, David Roche, Emilie Pateau, Ivan Dubois, Valérie A Delmas, Mélodie Cadillon, Madeleine Bouzon, Volker Döring   +8 more
doaj   +2 more sources

Protein Mass Effects on Formate Dehydrogenase. [PDF]

J Am Chem Soc, 2017
Isotopically labeled enzymes (denoted as "heavy" or "Born-Oppenheimer" enzymes) have been used to test the role of protein dynamics in catalysis. The original idea was that the protein's higher mass would reduce the frequency of its normal-modes without altering its electrostatics.
Ranasinghe C, Guo Q, Sapienza PJ, Lee AL, Quinn DM, Cheatum CM, Kohen A.   +6 more
europepmc   +4 more sources

Structure of recombinant formate dehydrogenase from Methylobacterium extorquens (MeFDH1) [PDF]

Scientific Reports
Formate dehydrogenase (FDH) is critical for the conversion between formate and carbon dioxide. Despite its importance, the structural complexity of FDH and difficulties in the production of the enzyme have made elucidating its unique physicochemical ...
Junsun Park, Yoonyoung Heo, Byoung Wook Jeon, Mingyu Jung, Yong Hwan Kim, Hyung Ho Lee, Soung-Hun Roh   +6 more
doaj   +2 more sources