Results 91 to 100 of about 39,076 (213)

Membrane electrochemical reactors (MER) for NADH regeneration in HLADH-catalysed synthesis: comparison of effectiveness [PDF]

, 2004
Two membrane electrochemical reactors(MER) were designed and applied to HLADH-catalysed reduction of cyclohexanone to cyclohexanol. The regeneration of the cofactor NADH was ensured electrochemically, using either methyl viologen or a rhodium complex as
Basséguy, Régine, Bergel, Alain, Délécouls-Servat, Karine   +2 more
core   +3 more sources

Mass Spectrometry Structural Proteomics Enabled by Limited Proteolysis and Cross‐Linking

Mass Spectrometry Reviews, EarlyView.
ABSTRACT The exploration of protein structure and function stands at the forefront of life science and represents an ever‐expanding focus in the development of proteomics. As mass spectrometry (MS) offers readout of protein conformational changes at both the protein and peptide levels, MS‐based structural proteomics is making significant strides in the
Haiyan Lu, Zexin Zhu, Lauren Fields, Hua Zhang, Lingjun Li   +4 more
wiley   +1 more source

A multienzyme system immobilized on surface-modified metal–organic framework for enhanced CO2 hydrogenation

Carbon Resources Conversion
Hydrogenating carbon dioxide to formate using formate dehydrogenase (FDH) is a sustainable approach for CO2 mitigation. Herein, we developed a biocatalytic system with cofactor regeneration by immobilizing multiple enzymes, namely FDH, carbonic anhydrase
Shadeera Rouf, Yaser E. Greish, Bart Van der Bruggen, Sulaiman Al-Zuhair   +3 more
doaj   +1 more source

Plant Formate Dehydrogenase

, 2005
The research in this study identified formate dehydrogenase, an enzyme that plays a metabolic role on the periphery of one-carbon metabolism, has an unusual localization in Arabidopsis thaliana and that the enzyme has an unusual kinetic plasticity. These properties make it possible that this enzyme could be engineered to attempt to engineer plants with
openaire   +3 more sources

Formation of 1-Deoxy-ketoses by Pyruvate Dehydrogenase and Acetoin Dehydrogenase [PDF]

Agricultural and Biological Chemistry, 1986
Cell-free extracts of Bacillus subtilis contain enzyme activities which catalyze an acyloin-type condensation reaction (carboligase reaction) resulting in the formation of 1-deoxy-ketoses. The reactions are deduced to proceed as follows: pyruvate + aldose →C02 + 1-deoxy-ketose    (I)acetoin + aldose →acetaldehyde + 1-deoxy-ketose   (II)methylacetoin ...
Akira YOKOTA, Ken-ichi SASAJIMA
openaire   +2 more sources

Glycine decarboxylase deficiency causes neural tube defects and features of non-ketotic hyperglycinemia in mice. [PDF]

, 2015
Glycine decarboxylase (GLDC) acts in the glycine cleavage system to decarboxylate glycine and transfer a one-carbon unit into folate one-carbon metabolism. GLDC mutations cause a rare recessive disease non-ketotic hyperglycinemia (NKH).
Brosnan, JT, Brosnan, ME, Copp, AJ, Greene, ND, Heales, S, Hutchin, T, Leung, KY, Pai, YJ, Prunty, H, Savery, D   +9 more
core   +1 more source

Metabolomic Insights Into Saliva as a Non‐Invasive Surrogate for Serum to Enable Community‐Based Biomarker Investigation

The Prostate, EarlyView.
ABSTRACT Introduction Using serum metabolomics in Community‐Based Participatory Research (CBPR) with underserved populations presents challenges due to the invasive nature of venipuncture, which limits longitudinal sample collection in community settings. In this pilot study, we compared saliva and serum metabolomics in patients with prostate cancer to
Alireza Abdshah, Mohammad Alyamani, Robert A. Burgess, Dipen J. Parekh, Benjamin Spieler, Rakesh Singal, Matthew C. Abramowitz, Jaime R. Merchan, Lucia Pannunzio, Brandon A. Mahal, Erin Kobetz, Sanoj Punnen, Marijo Bilusic, Nima Sharifi   +13 more
wiley   +1 more source

Metabolic deficiences revealed in the biotechnologically important model bacterium Escherichia coli BL21(DE3). [PDF]

PLoS ONE, 2011
The Escherichia coli B strain BL21(DE3) has had a profound impact on biotechnology through its use in the production of recombinant proteins. Little is understood, however, regarding the physiology of this important E. coli strain. We show here that BL21(
Constanze Pinske, Markus Bönn, Sara Krüger, Ute Lindenstrauss, R Gary Sawers   +4 more
doaj   +1 more source

Sequential Optimization of Multivariate Metal–Organic Framework Based Biocatalysis

Small, EarlyView.
A sequential Latin hypercube sampling–coupled Bayesian optimization (LHS‐BO) workflow integrates multivariate zirconium‐based metal–organic framework based biocomposite (E‐MOF) design with enzyme biocascade reaction optimization. The optimized E‐MOFs stabilize glucose oxidase and horseradish peroxidase in bioactive conformation, exhibiting high ...
Weibin Liang, Sisi Zheng, Ross Andrew Shalliker, Christian Doonan, Feng Li   +4 more
wiley   +1 more source

Biocatalytic cofactor regeneration for CO2 reduction: Integration of a hydrogenase and a formate dehydrogenase in H2-driven systems

Journal of CO2 Utilization
Formate dehydrogenases catalyze the reversible oxidation of formate to carbon dioxide. These enzymes play an important role in CO2 reduction and serve as nicotinamide cofactor recycling enzymes.
Michael Groh, Elisabeth Lettau, Janna Schoknecht, Jan Liedtke, Lars Lauterbach, Silke Leimkühler   +5 more
doaj   +1 more source