Results 91 to 100 of about 9,358 (215)

Mass Spectrometry Structural Proteomics Enabled by Limited Proteolysis and Cross‐Linking

Mass Spectrometry Reviews, EarlyView.
ABSTRACT The exploration of protein structure and function stands at the forefront of life science and represents an ever‐expanding focus in the development of proteomics. As mass spectrometry (MS) offers readout of protein conformational changes at both the protein and peptide levels, MS‐based structural proteomics is making significant strides in the
Haiyan Lu, Zexin Zhu, Lauren Fields, Hua Zhang, Lingjun Li   +4 more
wiley   +1 more source

Plant Formate Dehydrogenase

, 2005
The research in this study identified formate dehydrogenase, an enzyme that plays a metabolic role on the periphery of one-carbon metabolism, has an unusual localization in Arabidopsis thaliana and that the enzyme has an unusual kinetic plasticity. These properties make it possible that this enzyme could be engineered to attempt to engineer plants with
openaire   +3 more sources

Formation of 1-Deoxy-ketoses by Pyruvate Dehydrogenase and Acetoin Dehydrogenase [PDF]

Agricultural and Biological Chemistry, 1986
Cell-free extracts of Bacillus subtilis contain enzyme activities which catalyze an acyloin-type condensation reaction (carboligase reaction) resulting in the formation of 1-deoxy-ketoses. The reactions are deduced to proceed as follows: pyruvate + aldose →C02 + 1-deoxy-ketose    (I)acetoin + aldose →acetaldehyde + 1-deoxy-ketose   (II)methylacetoin ...
Akira YOKOTA, Ken-ichi SASAJIMA
openaire   +2 more sources

Exploring the directionality of Escherichia coli formate hydrogenlyase:a membrane-bound enzyme capable of fixing carbon dioxide to organic acid [PDF]

, 2016
During mixed‐acid fermentation Escherichia coli produces formate, which is initially excreted out the cell. Accumulation of formate, and dropping extracellular pH, leads to biosynthesis of the formate hydrogenlyase (FHL) complex. FHL consists of membrane
Andrews, Ashkenazy, Baba, Bagramyan, Bagramyan, Bagramyan, Bassegoda, Batista, Begg, Bettenbrock, Bowman, Brandt, Brandt, Brandt, Böhm, Casadaban, Deplanche, Doberenz, Efremov, Efremov, Efremov, Euro, Frielingsdorf, Guest, Hakobyan, Hamilton, Hedderich, Hirst, Kelley, Khangulov, Kim, Knappe, Krasna, Kuniyoshi, Laemmli, Lim, Maeda, Marreiros, McDowall, McDowall, Nguyen, Nitschke, Pakes, Peters, Pinske, Pinske, Pinske, Pinske, Ripple, Rittmann, Romano, Rossmann, Sapra, Sargent, Sauter, Sawers, Sawers, Sawers, Schlindwein, Schuchmann, Stephenson, Stephenson, Suppmann, Thauer, Towbin, Trchounian, Trchounian, Vignais, Wang, Weiss, Woods, Wu, Wyborn, Zhu, Zickermann   +74 more
core   +1 more source

Metabolomic Insights Into Saliva as a Non‐Invasive Surrogate for Serum to Enable Community‐Based Biomarker Investigation

The Prostate, EarlyView.
ABSTRACT Introduction Using serum metabolomics in Community‐Based Participatory Research (CBPR) with underserved populations presents challenges due to the invasive nature of venipuncture, which limits longitudinal sample collection in community settings. In this pilot study, we compared saliva and serum metabolomics in patients with prostate cancer to
Alireza Abdshah, Mohammad Alyamani, Robert A. Burgess, Dipen J. Parekh, Benjamin Spieler, Rakesh Singal, Matthew C. Abramowitz, Jaime R. Merchan, Lucia Pannunzio, Brandon A. Mahal, Erin Kobetz, Sanoj Punnen, Marijo Bilusic, Nima Sharifi   +13 more
wiley   +1 more source

Metabolic network analysis reveals microbial community interactions in anammox granules. [PDF]

, 2017
Microbial communities mediating anaerobic ammonium oxidation (anammox) represent one of the most energy-efficient environmental biotechnologies for nitrogen removal from wastewater.
Bhattacharjee, Ananda S, Goel, Ramesh, Hamilton, Joshua J, Lawson, Christopher E, McMahon, Katherine D, Noguera, Daniel R, Wu, Sha   +6 more
core   +2 more sources

Sequential Optimization of Multivariate Metal–Organic Framework Based Biocatalysis

Small, EarlyView.
A sequential Latin hypercube sampling–coupled Bayesian optimization (LHS‐BO) workflow integrates multivariate zirconium‐based metal–organic framework based biocomposite (E‐MOF) design with enzyme biocascade reaction optimization. The optimized E‐MOFs stabilize glucose oxidase and horseradish peroxidase in bioactive conformation, exhibiting high ...
Weibin Liang, Sisi Zheng, Ross Andrew Shalliker, Christian Doonan, Feng Li   +4 more
wiley   +1 more source

Electroenzymatic CO2 Reduction Using Formate Dehydrogenase: Direct and Mediated Catalysis

ChemElectroChem
The electrocatalytic reduction of carbon dioxide (CO2) to formate by the enzyme formate dehydrogenase (FDH) makes use of the enzyme's observed reversibility, offering a promising strategy for the mitigation of CO2 and the production of value‐added ...
Navendu Paul, Isabel Moura, Luísa B. Maia, Cristina M. Cordas, Jose J. G. Moura   +4 more
doaj   +1 more source

A comparative genomic analysis of energy metabolism in sulfate reducing bacteria and archaea

Frontiers in Microbiology, 2011
The number of sequenced genomes of sulfate-reducing organisms (SRO) has increased significantly in the recent years, providing an opportunity for a broader perspective into the energy metabolism of such organisms.
Inês A. C. ePereira, Ana Raquel Ramos, Fabian eGrein, Marta Coimbra Marques, Sofia Marques Da Silva, Sofia Santos Venceslau   +5 more
doaj   +1 more source

Proteomic analysis reveals metabolic and regulatory systems involved in the syntrophic and axenic lifestyle of Syntrophomonas wolfei [PDF]

, 2015
Microbial syntrophy is a vital metabolic interaction necessary for the complete oxidation of organic biomass to methane in all-anaerobic ecosystems.
Bryan R. Crable, Cody S. Sheik, Gregory B. Hurst, Jessica R. Sieber, Lars Rohlin, Michael J. McInerney, Robert P. Gunsalus   +6 more
core   +1 more source