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Improved purification of Candida boidinii formate dehydrogenase
Bioseparation, 2000Formate dehydrogenase (FDH, EC 1.2.1.2) from Candida boidinii was purified to homogeneity. The two step procedure comprised anion exchange chromatography (2.9-fold purification, 85% step yield, elution with 35 mM KCl), followed by dye-ligand affinity chromatography on immobilized Cibacron Blue 3GA (1.4-fold purification, 75% step yield, elution with 0 ...
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Formate Dehydrogenase from Clostridium Thermoaceticum
1980Clostridium thermoaceticum is an obligate anaerobic and thermophilic bacterium. It ferments various sugars to acetate, which is the only product, and from one mole of hexose almost three moles of acetate are formed.1 The fermentation of glucose is via the Embden-Meyerhof glycolytic pathway to pyruvate.2 Half of the pyruvate is oxidized by pyruvate ...
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formate dehydrogenase (acceptor) 1.1.99.33
2013Dietmar Schomburg, Ida Schomburg
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Purification and Some Properties of Formate Dehydrogenase
The Journal of Biochemistry, 1974T, Oyama, I, Yamazaki
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