Results 31 to 40 of about 9,358 (215)

The genetic basis of energy conservation in the sulfate-reducing bacterium Desulfovibrio alaskensis G20. [PDF]

, 2014
Sulfate-reducing bacteria play major roles in the global carbon and sulfur cycles, but it remains unclear how reducing sulfate yields energy. To determine the genetic basis of energy conservation, we measured the fitness of thousands of pooled mutants of
Arkin, Adam P, Bauer, Stefan, Deutschbauer, Adam M, Kuehl, Jennifer V, Price, Morgan N, Ray, Jayashree, Wetmore, Kelly M   +6 more
core   +1 more source

High yield 1,3-propanediol production by rational engineering of the 3-hydroxypropionaldehyde bottleneck in Citrobacter werkmanii [PDF]

, 2016
Background: Imbalance in cofactors causing the accumulation of intermediates in biosynthesis pathways is a frequently occurring problem in metabolic engineering when optimizing a production pathway in a microorganism.
Avcı, Fatma Gizem, Beauprez, Joeri, De Maeseneire, Sofie, De Mey, Marjan, Maervoet, Veerle, Soetaert, Wim   +5 more
core   +3 more sources

Selenium—More than Just a Fortuitous Sulfur Substitute in Redox Biology

Molecules, 2023
Living organisms use selenium mainly in the form of selenocysteine in the active site of oxidoreductases. Here, selenium’s unique chemistry is believed to modulate the reaction mechanism and enhance the catalytic efficiency of specific enzymes in ways ...
Luisa B. Maia, Biplab K. Maiti, Isabel Moura, José J. G. Moura   +3 more
doaj   +1 more source

Direct Biocatalytic Processes for CO₂ Capture as a Green Tool to Produce Value-Added Chemicals

Molecules, 2023
Direct biocatalytic processes for CO2 capture and transformation in value-added chemicals may be considered a useful tool for reducing the concentration of this greenhouse gas in the atmosphere.
Rocio Villa, Susana Nieto, Antonio Donaire, Pedro Lozano   +3 more
doaj   +1 more source

The respiratory molybdo-selenoprotein formate dehydrogenases of Escherichia coli have hydrogen: benzyl viologen oxidoreductase activity

BMC Microbiology, 2011
Background Escherichia coli synthesizes three membrane-bound molybdenum- and selenocysteine-containing formate dehydrogenases, as well as up to four membrane-bound [NiFe]-hydrogenases.
Trchounian Armen, Trchounian Karen, Ihling Christian, Waclawek Mandy, Kuhns Martin, Pinske Constanze, Soboh Basem, Sinz Andrea, Sawers Gary   +8 more
doaj   +1 more source

Biocatalysis as Useful Tool in Asymmetric Synthesis: An Assessment of Recently Granted Patents (2014–2019) [PDF]

, 2019
The broad interdisciplinary nature of biocatalysis fosters innovation, as different technical fields are interconnected and synergized. A way to depict that innovation is by conducting a survey on patent activities.
Alcántara, Andrés R., Domínguez de María, Pablo, Gonzalo Calvo, Gonzalo de   +2 more
core   +3 more sources

The Beta Subunit of Non-bifurcating NADH-Dependent [FeFe]-Hydrogenases Differs From Those of Multimeric Electron-Bifurcating [FeFe]-Hydrogenases

Frontiers in Microbiology, 2020
A non-bifurcating NADH-dependent, dimeric [FeFe]-hydrogenase (HydAB) from Syntrophus aciditrophicus was heterologously produced in Escherichia coli, purified and characterized.
Nathaniel A. Losey, Saroj Poudel, Eric S. Boyd, Michael J. McInerney   +3 more
doaj   +1 more source

Expression of Novel L-Leucine Dehydrogenase and High-Level Production of L-Tert-Leucine Catalyzed by Engineered Escherichia coli

Frontiers in Bioengineering and Biotechnology, 2021
Leucine dehydrogenase (LDH) is a NAD+-dependent oxidoreductase, which can selectively catalyze α-keto acids to obtain α-amino acids and their derivatives. It plays a key role in the biosynthesis of L-tert-leucine (L-Tle).
Yuan-Yuan Jia, Yuan-Yuan Jia, Yu-Li Xie, Yu-Li Xie, Lu-Lu Yang, Lu-Lu Yang, Hong-Ling Shi, Hong-Ling Shi, Yun-Feng Lu, Yun-Feng Lu, Si-Pu Zhang, Cun-Duo Tang, Cun-Duo Tang, Lun-Guang Yao, Lun-Guang Yao, Yun-Chao Kan   +15 more
doaj   +1 more source

Formate Dehydrogenase from Clostridium acidiurici [PDF]

Journal of Bacteriology, 1972
Partial purification of formate dehydrogenase from Clostridium acidiurici has been accomplished, and some properties of the enzyme have been determined. The molecular weight of the protein is at least 200,000 daltons.
J J, Kearny, R D, Sagers
openaire   +2 more sources

Expression of multidrug transporter P‐glycoprotein in Pichia pastoris affects the host's methanol metabolism

Microbial Biotechnology, 2019
Summary Pichia pastoris KM71H (MutS) is an efficient producer of hard‐to‐express proteins such as the membrane protein P‐glycoprotein (Pgp), an ATP‐powered efflux pump which is expressed properly, but at very low concentration, using the conventional ...
Wan‐cang Liu, Fei Zhou, Di Xia, Joseph Shiloach   +3 more
doaj   +1 more source