Results 141 to 150 of about 2,008,011 (306)

外凸台内齿圈件双工序反挤压成形工艺研究

open access: yesJixie chuandong, 2010
Rigid-visco-plastic finite element method is used to analyze precision forming process of the external boss and ring gear. And the variation of extruding forces,stress,strain as well as metal flow behavior was obtained. There was a good agreement between
练毅, 王平, 刘德仿, 廖萍
doaj  

Single‐molecule DNA flow‐stretch assays for high‐throughput DNA–protein interaction studies

open access: yesFEBS Open Bio, EarlyView.
We describe an optimised single‐molecule DNA flow‐stretch assay that visualises DNA–protein interactions in real time. Linear DNA fragments are tethered to a surface and stretched by buffer flow for fluorescence imaging. Using λ and φX174 DNA, this protocol enhances reproducibility and accessibility, providing a versatile approach for studying diverse ...
Ayush Kumar Ganguli   +8 more
wiley   +1 more source

Analysing the significance of small conformational changes and low occupancy states in serial crystallographic data

open access: yesFEBS Open Bio, EarlyView.
This protocol paper outlines methods to establish the success of a time‐resolved serial crystallographic experiment, by means of statistical analysis of timepoint data in reciprocal space and models in real space. We show how to amplify the signal from excited states to visualise structural changes in successful experiments.
Jake Hill   +4 more
wiley   +1 more source

Modeling of building structures when transferring dynamic effects through the soil mass

open access: yesNauka ta progres transportu, 2011
Factors influenced on strength parameters of constructions of exploiting buildings in difficult engineergeological conditions are analysed. Influence of dynamic forces, including forces from a public transport on the exploiting building is shown.
V.A. Banakh
doaj  

History of the United States Army Forces, Central Canada.

open access: yes, 2010
The history of the United States Army Forces in Central Canada is presented here. The body of the history deals with the growth and establishment of the Headquarters of the Command in Winnipeg, Manitoba, Canada.
Headquarters, United States Armed Forces, Central Canada
core  

Sudden anaerobization in Amphibacillus xylanus increases intracellular labile ferrous iron and inhibits cell growth

open access: yesFEBS Open Bio, EarlyView.
Abruptly changing from aerobic to anaerobic conditions (sudden anaerobization) induced growth inhibition and a significant increase in intracellular labile ferrous iron in the aerotolerant anaerobe Amphibacillus xylanus. We found that free flavins mediate efficient electron transfer from NADH to ferric iron under anaerobic conditions, suggesting that ...
Shinya Kimata   +13 more
wiley   +1 more source

Versatile vector tools for efficient protein screening across multiple expression systems

open access: yesFEBS Open Bio, EarlyView.
A unified vector toolkit enables rapid protein expression screening across E. coli, insect, and mammalian cells. A single primer pair amplifies the target gene, which is inserted into any vector via a standardized interface. This streamlined workflow eliminates repeated cloning steps, accelerating the identification of optimal expression conditions for
Zhimin Zhu   +5 more
wiley   +1 more source

UiO‐66 metal–organic frameworks in biomedicine: From structural tunability to bioimaging, photodiagnostics, and photodynamic cancer therapy

open access: yesFEBS Open Bio, EarlyView.
UiO‐66(Zr) metal–organic frameworks are chemically stable, biocompatible, and highly tunable nanomaterials. Their modular structure enables controlled drug delivery, multimodal bioimaging, and light‐activated photodynamic therapy, supporting integrated diagnostic and therapeutic (theranostic) applications in cancer and biomedical research.
Veronika Huntošová   +2 more
wiley   +1 more source

Airborne command and center. Army Ground Forces study no. 25.

open access: yes, 2020
A history of the Airborne Command and Center was compiled by various hands during the course of the war. This was completely reworks by the author, Lt. Col. John T.
Historical Section, Army Ground Forces; Ellis, John T., Jr.
core  

Hydrostatic pressure activates HIF‐1α via β‐catenin to promote stemness in breast cancer cells

open access: yesFEBS Open Bio, EarlyView.
To mimic the elevated intestinal fluid pressure in breast cancers, we loaded human breast cancer cells (MCF‐7, MDA‐MB‐453, and BT‐474) to 50 mmHg hydrostatic pressure. Hydrostatic pressure exposure upregulated HIF‐1α and induced stemness in MCF‐7 and BT‐474 cells.
Da Zhai   +8 more
wiley   +1 more source

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