Results 101 to 110 of about 21,639 (263)

Promiscuous stimulation of HSP70 ATPase activity by parasite‐derived J‐domains

FEBS Open Bio, EarlyView.
The malaria parasite Plasmodium falciparum exports three highly homologous yet functionally divergent J‐domain proteins into human erythrocytes. Here, we show that J‐domains isolated from all three proteins effectively stimulate the ATPase activity of both endogenous host and exported parasite HSP70 chaperones.
Julian Barth, Moritz Koch, Le‐Han Rössner, Johanna Eichhorn, Denys Pogoryelov, Matthias P. Mayer, Jude M. Przyborski   +6 more
wiley   +1 more source

Fractional Order Systems:PID Controller Design and Fractional Order Element Modeling

, 2014
This research thesis within the XXVI Ph.D. Course in Systems Engineering is centered on Fractional, or also noticed as Non-Integer Order, Systems. The Ph.D. Thesis' purpose is to present the auto-tuning procedure of Fractional Order PID Controllers PID proposing also a future implementation via fractional order elements. The Thesis is organized in four
openaire   +2 more sources

Single‐molecule DNA flow‐stretch assays for high‐throughput DNA–protein interaction studies

FEBS Open Bio, EarlyView.
We describe an optimised single‐molecule DNA flow‐stretch assay that visualises DNA–protein interactions in real time. Linear DNA fragments are tethered to a surface and stretched by buffer flow for fluorescence imaging. Using λ and φX174 DNA, this protocol enhances reproducibility and accessibility, providing a versatile approach for studying diverse ...
Ayush Kumar Ganguli, Mohammad Nour Alsamsam, Ugnė Bagdonaitė, Van Truc Vu, Chun‐Jen Huang, Polina Kuzhir, Mindaugas Zaremba, Aurimas Kopūstas, Marijonas Tutkus   +8 more
wiley   +1 more source

A Proportional-Integral-One Plus Double Derivative Controller-Based Fractional-Order Kepler Optimizer for Frequency Stability in Multi-Area Power Systems with Wind Integration

Fractal and Fractional
This study proposes an enhanced Kepler Optimization (EKO) algorithm, incorporating fractional-order components to develop a Proportional-Integral-First-Order Double Derivative (PI–(1+DD)) controller for frequency stability control in multi-area power ...
Mohammed H. Alqahtani, Sulaiman Z. Almutairi, Ali S. Aljumah, Abdullah M. Shaheen, Ghareeb Moustafa, Attia A. El-Fergany   +5 more
doaj   +1 more source

YlmG1 is localized exclusively to the chloroplast envelope membrane and is involved in preprotein translocation in Arabidopsis thaliana

FEBS Open Bio, EarlyView.
Cytosolically synthesized chloroplast preproteins are translocated across the outer and inner envelope membranes through translocons called TOC and TIC, respectively. In green algae and plants, the TIC core is composed of essential membrane proteins, Tic12, Tic20, and Tic214.
Mengyi Li, Xueyang Zhao, Masato Nakai
wiley   +1 more source

Proteasomal degradation of intracellularly expressed Amblyomin‐X limits suicide gene therapy potential in melanoma cells

FEBS Open Bio, EarlyView.
This study explores the feasibility of expressing the antitumoral protein Amblyomin‐X through a suicide gene therapy approach and investigates its intracellular fate after gene delivery. Although the gene is efficiently expressed, melanoma cells rapidly degrade the Amblyomin‐X protein via proteasome activity.
Victor Dal Posolo Cinel, Carlos DeOcesano Pereira, Aline Ramos Maia Lobba, Melissa Regina Fessel, Ana Marisa Chudzinski‐Tavassi   +4 more
wiley   +1 more source

Finite element method for fractional order parabolic obstacle problem with nonlinear source term

Partial Differential Equations in Applied Mathematics
This paper considers the finite element method for a fractional order parabolic obstacle problem in time with a nonlinear source term. An error estimation of the spatially semi-discrete problem is discussed.
Allaoua Mehri, Hakima Bouhadjera, Mohammed S. Abdo, Hadeel Z. Alzumi, Wafa Shammakh   +4 more
doaj   +1 more source

Rapid screening of staphylokinase protein variants using an unpurified cell‐free expression system

FEBS Open Bio, EarlyView.
An unpurified cell‐free protein synthesis (CFPS) platform enables rapid functional screening of staphylokinase variants. Direct plasminogen‐activation assays performed in microplate format provide real‐time activity readouts, allowing rapid identification and ranking of variants with improved or reduced fibrinolytic activity without protein ...
Maria Tomková, Veronika Hovanová, Jiri Damborsky, Erik Sedlák   +3 more
wiley   +1 more source

Fractional-order elements (FOEs) using minimax approximation

, 2021
A MATLAB program for fractional-order elements (FOEs) calculations using minimax approximation. A companion with the paper N. Mijat, D. Jurisic, and G. S. Moschytz, "Analog Modeling of Fractional-order Elements: A Classical Circuit Theory Approach, " IEEE Access, 2021, DOI 10.1109/ACCESS.2021 ...
openaire   +2 more sources

Evolutionarily divergent DUF4465 domains have a common vitamin B12‐binding function

FEBS Open Bio, EarlyView.
We show that DUF4465 family proteins, widespread across bacteria from gut microbiomes, hydrothermal vents, and soil, share a common vitamin B12‐binding function. These augmented β‐jellyroll proteins bind vitamin B12 via extended loops. Our findings establish sequence‐diverse DUF4465 proteins as a widespread class of B12‐binding proteins, highlighting ...
Charlea Clarke, Michal Banasik, Rokas Juodeikis, Martin J. Warren, Richard W. Pickersgill   +4 more
wiley   +1 more source