Results 101 to 110 of about 8,952,987 (279)

Protocol for quantifying miRNA trafficking across the endosomal membrane

open access: yesFEBS Open Bio, EarlyView.
An in vitro protocol measures miRNA uptake into endosomes isolated from mammalian cell extracts, which are free of subcellular contaminants. Performed at 37 °C in the presence of ATP, it ensures the import of single‐stranded miRNA into the endosomal lumen.
Syamantak Ghosh   +2 more
wiley   +1 more source

Identification of gastrointestinal parasites and their effects on blood parameters in free-range chickens

open access: yesJournal of Microbiology and Infectious Diseases
Background: Gastrointestinal (GIT) parasites can reduce poultry productivity by negatively affecting the birds' health and altering specific blood values.
Nagia A. S. Abdalsalam   +2 more
doaj   +1 more source

Heterotropic regulation and negative homotropic cooperativity

open access: yesFEBS Open Bio, EarlyView.
We identified a structural module common to some proteins that couple negative cooperativity with heterotropic regulation, two features that rarely coexist. These proteins are ring‐like and present an ordered asymmetry whereby noncontacting subunits are symmetric, and their tertiary structure differs from that of contacting subunits.
Veronica Morea   +5 more
wiley   +1 more source

Identification of Chlamydia gallinacea in a parrot and in free‐range chickens in Australia

open access: yesAustralian Veterinary Journal, 2019
Chlamydia gallinacea is a recently described bacterial species in a genus known to infect and cause disease in animals and humans. Our report describes the identification of C. gallinacea infection in free‐range laying chickens (Gallus gallus) in Australia, and the identification of C. gallinacea infection in a parrot, a wild Australian galah (Eolophus
H S, Stokes   +6 more
openaire   +3 more sources

Structural and biochemical insights into the thermostable esterase Ta0887 from Thermoplasma acidophilum

open access: yesFEBS Open Bio, EarlyView.
In this study, a novel esterase from the thermoacidophilic archaeon Thermoplasma acidophilum was biochemically and structurally characterized. Our results demonstrate that Ta0887 is a highly thermostable esterase that preferentially hydrolyzes p‐nitrophenyl hexanoate and possesses an α‐helical cap domain that likely contributes to its substrate ...
Alejandro Delgado‐Rey   +4 more
wiley   +1 more source

Investigation of the ability to form biofilms in-vitro in sanitary-indicatory bacteria isolated from chickens

open access: yesUkrainian Journal of Veterinary Sciences
Biofilms provide resistance to antimicrobial agents and the body’s immune response in microorganisms that colonise the digestive tract of animals, in particular poultry.
L. Davydovska   +4 more
doaj   +1 more source

Recent insights into the molecular mechanism of ubiquinol oxidation by cytochrome bc1

open access: yesFEBS Open Bio, EarlyView.
The review reflects on the mechanism of the catalytic reaction in cytochrome bc1: the electron bifurcation that involves the separation of two electrons derived from the quinol oxidation to opposite sides of the enzyme across the membrane. This review summarizes the long‐standing effort to understand the mechanism of quinol oxidation catalyzed by ...
Anna Wójcik‐Augustyn   +2 more
wiley   +1 more source

Effect of season and farming system on the quantity and nutritional quality of scavengeable feed resources and performance of village poultry in central Tanzania [PDF]

open access: yes, 2008
2 x 2 factorial study was conducted to assess the effect of season and farming system on the quantity and nutritional quality of scavengeable feed resources and the performance of village poultry in central Tanzania.
Goromela, E.H.   +3 more
core  

Occurrence of gastrointestinal helminthes in commercial and free-range chickens in Gaza Strip, Palestine [PDF]

open access: yes, 2021
The present study compared the prevalence of helminthes parasites in two chicken breeds in Gaza strip, the commercial and the free-range indigenous. Gastrointestinal tracts of 100 commercial growers and 90 free-range indigenous chickens collected from ...
Rayyan, Abdelnasser   +4 more
core  

IGF2 knockout reduces but does not abolish osteosarcoma growth in vitro and in vivo

open access: yesFEBS Open Bio, EarlyView.
To test whether endogenous IGF2 promotes osteosarcoma growth, IGF2 was knocked out in Saos2 cells via CRISPR‐Cas9. KO cells showed reduced proliferation in vitro, and knockout xenografts in mice reached only ~25% of wild‐type tumor volume. Insulin‐like growth factor 2 (IGF2) is implicated in osteosarcoma, but direct functional evidence of its role is ...
Shun Yao, Marco Archetti
wiley   +1 more source

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