Results 61 to 70 of about 2,661,924 (301)

Single‐molecule DNA flow‐stretch assays for high‐throughput DNA–protein interaction studies

FEBS Open Bio, EarlyView.
We describe an optimised single‐molecule DNA flow‐stretch assay that visualises DNA–protein interactions in real time. Linear DNA fragments are tethered to a surface and stretched by buffer flow for fluorescence imaging. Using λ and φX174 DNA, this protocol enhances reproducibility and accessibility, providing a versatile approach for studying diverse ...
Ayush Kumar Ganguli, Mohammad Nour Alsamsam, Ugnė Bagdonaitė, Van Truc Vu, Chun‐Jen Huang, Polina Kuzhir, Mindaugas Zaremba, Aurimas Kopūstas, Marijonas Tutkus   +8 more
wiley   +1 more source

Mass spectrometry based identification of AMP‐O‐Tris generated by Thermococcus onnurineus Cas10

FEBS Open Bio, EarlyView.
Isolated Thermococcus onnurineus Cas10 generates the noncanonical ATP‐derived product AMP‐O‐Tris while in Tris‐containing buffer as identified via mass spectrometry, revealing relaxed nucleophile selectivity under isolated conditions. These findings suggest that multiprotein Csm complex assembly restricts Cas10 reactivity toward canonical cyclic ...
Su‐Jin Lee, Ga Seul Lee, JinHan Kim, Kwang‐Hyun Park, Seong‐Ryung Go, Jeong Hee Moon, Eui‐Jeon Woo   +6 more
wiley   +1 more source

Fluid characteristics, gas accumulation controlling factors and gas enrichment modes in coal reservoirs: A case study of the Upper Paleozoic in the central-eastern Ordos Basin, NW China

Petroleum Exploration and Development
Based on the test and experimental data from exploration well cores of the Upper Paleozoic in the central-eastern Ordos Basin, combined with structural, burial depth and fluid geochemistry analyses, this study reveals the fluid characteristics, gas ...
Shida CHEN, Dazhen TANG, Wei HOU, Daojun HUANG, Yongzhou LI, Jianling HU, Hao XU, Shu TAO, Song LI, Shuling TANG   +9 more
doaj   +1 more source

Molecular dynamics simulations of positively selected codons in FcγRI reveal novel biochemical binding properties

FEBS Open Bio, EarlyView.
Evolutionary analysis across 32 placental mammals identified positive selection at residues H148 and W149 in the immune receptor FcγR1. Ancestral reconstruction combined with molecular dynamics simulations reveals how these mutations may influence receptor structure and dynamics, providing insight into the evolution of antibody recognition and immune ...
David A. Young, Omar Arias‐Gaguancela, Fiona Gibson, Morgan Grainger, Jodie Score, Amit Nathubhai, Mauricio Cafiero, Lee Richard Machado   +7 more
wiley   +1 more source

Cosmic rays and the primordial gas

, 2007
One of the most outstanding problems in the gravitational collapse scenario of early structure formation is the cooling of primordial gas to allow for small mass objects to form.
Abel, Abel, Aldrovandi, Bakker, Benedetta Ciardi, Biermann, Black, Cen, Cen, Ciardi, Dalgarno, De Jong, Diehl, Donahue, Dove, Enßlin, Galli, Ginzburg, Glover, Gould, Groom, Haiman, Haiman, Hartquist, Heitler, Hirata, Janev, Jasche, Jens Jasche, Karpas, Mannheim, Martin, Miniati, Oneil, Osterbrock, Osterbrock, Peebles, Peskin, Pfrommer, Ripamonti, Roberge, Shapiro, Shchekinov, Shull, Smith, Smith, Spitzer, Spitzer, Stanev, Sternheimer, Tegmark, Thielens, Torsten A. Enßlin, Weiss, Whaling, Wise, Yousif, Ziegler   +57 more
core   +2 more sources

Acute caffeine treatment protects the developing retina from ischemia‐induced cell death

FEBS Open Bio, EarlyView.
Caffeine reduces cell death in the developing retina under ischemia (OGD). This effect does not involve BDNF upregulation or antioxidant pathways (NRF2/VEGF). Neuroprotection occurs mainly through adenosine A2A receptor antagonism, decreasing glutamate release and excitotoxicity, highlighting caffeine's potential as an acute neuroprotective agent in ...
Amanda Alves Nascimento, Danniel Pereira‐Figueiredo, Gabriella Grossi de Lima Dias, Gabriel Ferreira dos Santos, Mariana Rodrigues Pereira, Rafael Brito, Karin C Calaza   +6 more
wiley   +1 more source

Insights into the Process of Gas Release from Organic-Rich Shale: Release Characteristics and Controlling Factors

Geofluids, 2023
Shale gas release in canister can significantly enrich our understanding in gas-in-place characteristics. However, studies on shale gas release characteristics and its controlling factors are rare.
Fei Li, Wei Dang, Fengqin Wang, Haikuan Nie, Yubo Feng, Qiubo Liu, Jiangtao Sun, Yao Ma   +7 more
doaj   +1 more source

Correlations in a confined magnetized free-electron gas

, 2001
Equilibrium quantum statistical methods are used to study the pair correlation function for a magnetized free-electron gas in the presence of a hard wall that is parallel to the field.
Abramowitz M, Alastuey A, Alastuey A, Balian R, Bohr N, Bohr N, Cornu F, Erdélyi A, Erdélyi A (ed), Erdélyi A (ed), Ginibre J, Jancovici B, Jancovici B, John P, Kettenis M M, Kettenis M M, L G Suttorp, Landau L, M M Kettenis, Macris N, Macris N, Magnus W, Ohtaka K, Roepstorff R, Sondheimer E H, Van Leeuwen H J, Van Leeuwen H J, Wong R   +27 more
core   +3 more sources

Sudden anaerobization in Amphibacillus xylanus increases intracellular labile ferrous iron and inhibits cell growth

FEBS Open Bio, EarlyView.
Abruptly changing from aerobic to anaerobic conditions (sudden anaerobization) induced growth inhibition and a significant increase in intracellular labile ferrous iron in the aerotolerant anaerobe Amphibacillus xylanus. We found that free flavins mediate efficient electron transfer from NADH to ferric iron under anaerobic conditions, suggesting that ...
Shinya Kimata, Yoichi Sakai, Keisuke Tanaka, Etsuro Yoshimura, Ken Kitano, Morio Ishikawa, Tadahiro Nakamoto, Tsutomu Takayama, Hirofumi Inoue, Kaho Nomura, Tomonori Suzuki, Masataka Uchino, Akira Abe, Youichi Niimura   +13 more
wiley   +1 more source

Time‐restricted feeding prior to Mycobacterium tuberculosis infection reduces tissue CD4+ T cells with limited impact on bacterial clearance

FEBS Open Bio, EarlyView.
Time‐restricted feeding (TRF) in mice increased liver fatty acid oxidation and decreased fatty acid biosynthesis. These alterations persisted when TRF was discontinued and the host was infected with Mycobacterium tuberculosis. Pre‐exposure to TRF did not alter tissue (lung and spleen) mycobacterial burden but significantly reduced CD3+ T cells in lungs
Ashish Gupta, Nidhi Yadav, Subhasmita Das, R. Rajendra Kumar Reddy, Nupur Sharma, Amol Ratnakar Suryawanshi, Jaswinder Singh Maras, Ranjan Kumar Nanda   +7 more
wiley   +1 more source