Results 221 to 230 of about 1,276,041 (313)

Homologous expression and purification of human HAX‐1 for structural studies

FEBS Open Bio, EarlyView.
This research protocol provides detailed instructions for cloning, expressing, and purifying large quantities of the intrinsically disordered human HAX‐1 protein, N‐terminally fused to a cleavable superfolder GFP, from mammalian cells. HAX‐1 is predicted to undergo posttranslational modifications and to interact with membranes, various cellular ...
Mariana Grieben
wiley   +1 more source

Revisiting 3Rs: rethinking replacement and new approach methodologies. [PDF]

Front Toxicol
Rosolowski J, Weber T, Malakpour-Permlid A, Oredsson S.   +3 more
europepmc   +1 more source

Thrombolytic proteins profiling: High‐throughput activity, selectivity, and resistance assays

FEBS Open Bio, EarlyView.
We present optimized biochemical protocols for evaluating thrombolytic proteins, enabling rapid and robust screening of enzymatic activity, inhibition resistance, and fibrin affinity, stimulation, and selectivity. The outcome translates to key clinical indicators such as biological half‐life and bleeding risk. These assays streamline the development of
Martin Toul, Alan Strunga, Jiri Damborsky, Zbynek Prokop   +3 more
wiley   +1 more source

Tunable Skeletal Editing of Benzothiazole and Benzisothiazole Via Carbene Transfer Reactions. [PDF]

JACS Au
Cai BG, Tan X, Xu N, Chen ZL, Xie Y, Ye C, Luo G, Xuan J.   +7 more
europepmc   +1 more source

Molecular determinants of signal transduction in tropomyosin receptor kinases

FEBS Open Bio, EarlyView.
Tropomyosin receptor kinases control critical neuronal functions, but how do the same receptors produce diverse cellular responses? This review explores the structural mechanisms behind Trk signaling diversity, focusing on allosteric modulation and ligand bias.
Giray Enkavi
wiley   +1 more source

Fe₃O₄/cellulose/Zn-MOF: introduction of a new catalyst for the synthesis of tetrazolo[1,5-<i>a</i>]pyrimidine-6-carbonitriles and diphenyl-1,3-thiazoles under solvent-free conditions. [PDF]

RSC Adv
JahangirVazifeh M, Abdollahi-Basir MH, Sharifzadeh B, Shirini F.   +3 more
europepmc   +1 more source

Long‐term culture of skin biopsies: maintenance of fibroblast production and competency of reprogramming

FEBS Open Bio, EarlyView.
Skin biopsies taken from a patient with an ultra‐rare disorder as well as controls were cultured for up to 473 days. The chunks of skin were serially transferred to a new culture plate when confluent with fibroblasts. Different generations of fibroblasts were analyzed for cell and molecular properties, proliferation, and competence for reprogramming to
Sudiksha Rathan‐Kumar, Michael A. Ripperger, Grant M. Westlake, Kevin C. Ess   +3 more
wiley   +1 more source

Comparison of Nicotine and Selected Flavoring Contents Between Tobacco-Free and Tobacco-Containing Oral Pouches. [PDF]

Nicotine Tob Res
Page MK, Leigh NJ, Block AC, Marrano PH, Travers MB, Adrover JE, Maley GE, Koenig LA, Salem EM, Heldwein SD, Goniewicz ML.   +10 more
europepmc   +1 more source

Day/night variations of myeloid and lymphoid cell subsets in the murine inguinal lymph node

FEBS Open Bio, EarlyView.
The circadian system is involved in the temporal regulation of the immune system. Our study reveals that two innate immune populations, NKT cells and neutrophils, predominate at the beginning of the day in healthy mice, highlighting how the time of day influences immune responses.
Paula M. Wagner, César G. Prucca, Lucia Boffelli, Virginia A. Piqueras, Silvia G. Correa, Mariana Maccioni, Mario E. Guido   +6 more
wiley   +1 more source

Tobacco endgame goals and measures in Europe: current status and future directions. [PDF]

Tob Control
Ollila H, Ruokolainen O, Laatikainen T, Koprivnikar H, and JATC-2 WP9 co-authors.   +4 more
europepmc   +1 more source