Results 111 to 120 of about 1,519 (227)

Taking advantage of the use of supervised learning methods for characterization of sperm population structure related with freezability in the Iberian red deer

open access: yes, 2017
Using Iberian red deer as a model, this study presents a supervised learning method, the Support Vector Machines (SVM), to characterize sperm population structure related with freezability.
Soler, Ana J.   +7 more
core   +1 more source

Evaluating the effect of γ‐oryzanol on MASLD pathology using a medaka fish model

open access: yesFEBS Open Bio, EarlyView.
This study explores a liver disease called MASLD, which is increasing worldwide and can lead to serious damage. Researchers used medaka fish instead of rodents to test a food compound, γ‐oryzanol. Fish fed this compound had less liver fat and healthier gut bacteria.
Yukako Ito   +7 more
wiley   +1 more source

FREEZABILITY AND FERTILITY OF STALLION SEMEN

open access: yes, 2011
Not AvailableVariations in freezability of extended raw semen between individual stallions and rate of conception with cryopreserved semen were studied. Semen was collected through artificial vagina (AV) from 14 Marwari stallions (4 collections each) of ...
YASH PAL, A ARANGASAMY, R A LEGHA, J SINGH, R S BANSAL, S K KHURANA and S N TANDON
core  

Differential abundances of four forms of binder of SPerm 1 in the seminal plasma of Bos taurus indicus bulls with different patterns of semen freezability

open access: yes, 2016
The Binder of SPerm 1 (BSP1) protein is involved in the fertilization and semen cryopreservation processes and is described to be both beneficial and detrimental to sperm.
Santos, Thaís Ferreira do   +9 more
core   +1 more source

Structural studies and functional engineering of NanX: an anhydro‐sialic acid transporter from Escherichia coli

open access: yesFEBS Open Bio, EarlyView.
Biophysical characterisation shows that NanX, a membrane transport protein from the major facilitator superfamily (MFS), forms both monomers and dimers after purification. AlphaFold modelling and substrate docking provide information on residues likely involved in substrate recognition for NanX and another MFS member, NanT.
Michael C. Newton‐Vesty   +13 more
wiley   +1 more source

Purification and preparation of Marchantia polymorpha Auxin Response Factor 2 for phase separation studies

open access: yesFEBS Open Bio, EarlyView.
We describe detailed protocols for the purification and preparation of Marchantia polymorpha Auxin Response Factor 2 (MpARF2). This protein is fused to an MBP solubility tag and an mNG fluorescent tag and is purified from Escherichia coli. The presented procedures make it possible to study MpARF2 assemblies, which could arise from phase separation ...
Bas Janssen   +5 more
wiley   +1 more source

SOME FATTY ACID AND SEMEN CHARACTERISTICS OF HOLSTEIN BULLS AS INLUENCED BY DIFFERENT SPERM FREEZABALITY

open access: yesThe Iraqi Journal of Agricultural science
This study was conducted to investigate the relationship between the concentration of some fatty acids in seminal plasma and semen characteristics of Holstein bulls with different sperm freezabilities.
S. M. Eidan, R. I. Khalil, A.F.Naser
doaj   +1 more source

Protocol for quantifying miRNA trafficking across the endosomal membrane

open access: yesFEBS Open Bio, EarlyView.
An in vitro protocol measures miRNA uptake into endosomes isolated from mammalian cell extracts, which are free of subcellular contaminants. Performed at 37 °C in the presence of ATP, it ensures the import of single‐stranded miRNA into the endosomal lumen.
Syamantak Ghosh   +2 more
wiley   +1 more source

MARK4 enhances stress granule formation under oxidative stress and increases tau accumulation

open access: yesFEBS Open Bio, EarlyView.
MARK4 (red dots) localizes to stress granules (orange dots) and promotes their formation under oxidative stress by modulating TIA1 (blue dots). MARK4 and TIA1 synergistically increase tau (purple) accumulation, and the reduction of the TIA1 ortholog suppresses neurodegeneration in a fly model.
Sho Nakajima   +8 more
wiley   +1 more source

Identification and characterization of a gene conferring stress resistance in Escherichia coli and cyanobacteria

open access: yesFEBS Open Bio, EarlyView.
Functional screening identified PcSyn14890, a cyanobacteria‐specific protein that enhances growth and stress resistance in E. coli and Synechocystis PCC6803. Although we expected it to function as a molecular chaperone, it was unable to protect against thermal aggregation of GAPDH.
Akiyo Yamada   +7 more
wiley   +1 more source

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