Results 181 to 190 of about 2,998 (248)

Freeze-fracture-autoradiography

Histochemistry, 1976
A new method for the electron microscope autoradiography of soluble substances in frozen tissue is described. The basic features of the method are freeze fracturing, the application of a suitable monolayer followed by exposure at low temperature and finally the separation of tissue and the replica-monolayer-sandwich after photographic processing.
E, Rix, A, Schiller, R, Taugner
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Complementary freeze‐fracture, freeze‐etch specimens

Journal of Microscopy, 1979
SUMMARYA procedure is described for the preparation and comparison of complementary freeze‐fracture‐freeze‐etch specimens. These complementary replicas reveal the value of etching some specimens even when the cryoprotectant concentration amounts to as much as 25% glycerol and 25% sucrose.
R L, Steere, E F, Erbe
openaire   +2 more sources

Freeze Fracture and Freeze Etching

2013
Freeze fracture depends on the property of frozen tissues or cells, when cracked open, to split along the hydrophobic interior of membranes, thus revealing broad panoramas of membrane interior. These large panoramas reveal the three-dimensional contours of membranes making the methods well suited to studying changes in membrane architecture.
Douglas E, Chandler, William P, Sharp
openaire   +2 more sources

Freeze-fracture immunogold labeling

Histochemistry and Cell Biology, 1996
Several approaches have been developed to combine immunogold cytochemistry and freeze-fracture techniques. These methods are highly heterogeneous regarding both the sequence of the procedural steps and the aspect of the resulting images. They imply immunolabeling either before or after freeze-fracture or even immunolabeling of platinum/carbon replicas ...
TORRISI, Maria Rosaria   +1 more
openaire   +2 more sources

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