Results 191 to 200 of about 2,998 (248)

Freeze-Fracture Enzyme Cytochemistry: Application of Enzyme Cytochemistry to Freeze-Fracture Cytochemistry

Journal of Electron Microscopy, 1996
We present a novel freeze-fracture cytochemistry method based upon enzyme cytochemistry. By this method, freeze-fractured membranes are labeled with cerium as an enzyme cytochemical marker on replicas. The cerium capture method was suitable for freeze-fracture enzyme cytochemistry because the cerium phosphate reaction product is stable after replica ...
T, Takizawa, T, Saito
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A simple and reliable quick-freezing/freeze-fracturing procedure

Histochemistry and Cell Biology, 1997
We describe a simple method for the quick-freezing/freeze-fracturing of cells in tissues or culture monolayers. Tissue slices or cultured cells were covered with thin copper foil (10-micron-thick), and frozen by smashing them against a liquid helium-cooled copper block.
K, Fujimoto, T, Noda, T, Fujimoto
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Combined Freeze-Fracturing and Autoradiography Techniques: Freeze-Fracture Autoradiography

1981
In the past many efforts have been made to apply classical techniques of fixation and embedding to the autoradiography of soluble material. There is general agreement today that this goal can only be reached by cryotechniques (Amlacher 1974).
A. Schiller, R. Taugner
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Freeze‐fracture: A personal history

Journal of Electron Microscopy Technique, 1989
AbstractThe sequence of events leading to the development of freeze‐fracture replication is described. Subsequent developments discussed include complementary replicas, replica interpretation with stereo micrograph and reversal negatives, replica reinforcement, and control of resistance evaporation.
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Freeze-fracture electron microscopy

Nature Protocols, 2007
The freeze-fracture technique consists of physically breaking apart (fracturing) a frozen biological sample; structural detail exposed by the fracture plane is then visualized by vacuum-deposition of platinum-carbon to make a replica for examination in the transmission electron microscope.
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Freeze-Etching and Freeze-Fracturing

1973
The freeze-etching (or fracturing) technique involves the making of a platinum-carbon replica of the fracture face through frozen cells. This replica is examined in the electron microscope. There are two advantages compared with earlier techniques. Firstly, fixation and dehydration are not necessary and the replica is thus of cells which differ from ...
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Freeze‐fracture of manufactured foods

Journal of Electron Microscopy Technique, 1989
AbstractManufacture of food products with desired texture and stability increasingly benefits from an understanding of the food microstructure. Freeze‐fracture is advantageous in gaining this understanding because it avoids alterations of hydration, retains fat, and maximally preserves air cells.
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Complementary freeze-fracture and freeze-etch preparations

Proceedings, annual meeting, Electron Microscopy Society of America, 1984
Whereas freeze-etching technology was introduced in 1957 (1) and the first freeze-etching equipment to be made available commercially was developed in 1961 (2), the need for complementary replicas was not recognized until Branton (3) proposed that the two membrane faces seen in freeze-etched specimens were not the inner and outer surfaces of membranes ...
Russell L. Steere, Eric F. Erbe, Bryan K. Bartle   +2 more
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Freeze‐fracturing and freeze‐etching of cardiac myosin filaments

Journal of Microscopy, 1975
SUMMARYMyofilament structure was studied in freeze‐etch replicas of unfixed, glycerinated beef cardiac muscle. The information which is revealed depends upon the direction of metal shadowing in relation to the filament axis. Shadows oblique to this axis reveal that the outer surface of a longitudinal half of a thick filament comprises three, sometimes ...
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Molecular Cytochemistry of Freeze-Fractured Cells: Freeze-Etching, Fracture-Label, Fracture-Permeation, and Label-Fracture

Advances in Molecular and Cell Biology, 1987
Publisher Summary This chapter describes three alternative techniques for the cytochemical characterization of the components (membrane associated or not) that are exposed by freeze-fracture. The first, fracture-label permits the labeling of plasma and intracellular membranes (as well as cytoplasms and nucleoplasms) after freeze-fracture. The second,
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