Results 81 to 90 of about 4,794,485 (241)

Transformation frequency of hygromycin-resistant calli on regeneration medium.

open access: yes
Transformation frequency of hygromycin-resistant calli on regeneration medium.
Nasir Mehmood (6448181)   +9 more
core   +1 more source

Designation, characteristics and transformation frequency of plasmids tested in Lactobacillus jensenii ATCC 25258.

open access: yes, 2023
Designation, characteristics and transformation frequency of plasmids tested in Lactobacillus jensenii ATCC 25258.
Miguel Camacho Rufino (14642556)   +9 more
core   +1 more source

The Shewanella oneidensis Fic enzyme SoFic targets the switch‐I region of EF‐Tu for AMPylation

open access: yesFEBS Letters, EarlyView.
Fic enzymes mediate diverse post‐translational modifications across all domains of life, including AMPylation. Prokaryotic EF‐Tu can be AMPylated and deAMPylated by the conserved Fic enzyme SoFic. Structural and biochemical approaches were used to characterize the effect of AMPylation on EF‐Tu, SoFic's enzymatic activities, and the enzyme‐target ...
Svenja Runge   +6 more
wiley   +1 more source

Optimization of ultrasound-mediated DNA transfer for bacteria and preservation of frozen competent cells

open access: yesMicrobiology Spectrum
The transformation of DNA into cells is the basis of molecular biology. Commonly employed techniques include heat shock transformation, electro-transformation, conjugation, transduction, and protoplast fusion.
Meng Zhang   +8 more
doaj   +1 more source

Current limitations of cryogenic microwave oscillator frequency stability

open access: yes, 2014
Technical paperCryogenic microwave oscillators built upon sapphire loaded cavity resonators exhibit excellent levels of frequency stability. Here we present an overview of the current understanding of the various processes and noise sources that limit ...
Parker, S.   +7 more
core   +1 more source

Chiral separation of chloroalkanes with the chromatographic column onboard Martian rovers

open access: yesFEBS Letters, EarlyView.
Computer image of the Rosalind Franklin Rover of ESA's ExoMars mission. ExoMars is scheduled to land on planet Mars in Oxia Planum in 2029. This area represents an interesting spot to look for biosignatures. Investigations of ExoMars include measurement on molecular chirality. We show that chiral chloroalkanes, that have been identified on Mars, can be
Asma Merzougui   +4 more
wiley   +1 more source

Prospecting the protein design landscape

open access: yesFEBS Letters, EarlyView.
This review outlines the current state of various protein design approaches. We discuss the current possibilities enabled by recently released tools, highlight future avenues to pursue in protein design, and underscore the crucial role of key databases and resources for successful protein design workflows.
Jakob R. Riccabona   +4 more
wiley   +1 more source

The Efficiency of Arabidopsis thaliana Floral Dip Transformation Is Determined Not Only by the Agrobacterium Strain Used but Also by the Physiology and the Ecotype of the Dipped Plant

open access: yesMolecular Plant-Microbe Interactions, 2013
To evaluate the chromosomal background of different Agrobacterium strains on floral dip transformation frequency, eight wild-type Agrobacterium strains, provided by Laboratorium voor Microbiologie Gent (LMG) and classified in different genomic groups ...
Rim Ghedira   +3 more
doaj   +1 more source

Artificial molecular machines and motors—Design and control of nanoscale motion

open access: yesFEBS Letters, EarlyView.
Molecules are constantly moving because of thermal fluctuations, but random motion alone cannot be exploited to perform directional tasks. Artificial molecular machines use chemical, electrical, or light energy to bias this motion. Molecular shuttles, rotary motors, and supramolecular pumps illustrate how nanoscale movement can be controlled and ...
Leonardo Andreoni, Alberto Credi
wiley   +1 more source

Perturbation of H3K27me3-Associated Epigenetic Processes Increases Agrobacterium-Mediated Transformation

open access: yesMolecular Plant-Microbe Interactions, 2017
Agrobacterium-mediated transformation is a core technology for basic plant science and agricultural biotechnology. Improving transformation frequency is a major goal for plant transgenesis. We previously showed that T-DNA insertions in some histone genes
Hidekazu Iwakawa   +4 more
doaj   +1 more source

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