Results 91 to 100 of about 229,738 (330)
Analytical Chemistry, 2019 The hybridization chain reaction (HCR) is a simple and sensitive method for quantifying nucleic acids. Current approaches cannot combine a washing-free sensing format with multiplexed target quantification at low concentrations, which would be highly ...
Jiajia Guo +3 more
semanticscholar +1 more source
Laser‐Induced Graphene from Waste Almond Shells
Advanced Functional Materials, EarlyView.Almond shells, an abundant agricultural by‐product, are repurposed to create a fully bioderived almond shell/chitosan composite (ASC) degradable in soil. ASC is converted into laser‐induced graphene (LIG) by laser scribing and proposed as a substrate for transient electronics.
Yulia Steksova +9 more
wiley +1 more source
A sensitive ERK fluorescent probe reveals the significance of minimal EGF-induced transcription
Cell Structure and FunctionExtracellular signal-regulated kinase (ERK) regulates multiple cellular functions through distinct activation patterns. Genetically encoded fluorescent probes are instrumental in dissecting the ERK activity dynamics in living cells.
Zhang Weisheng +4 more
doaj +1 more source
Advanced Functional Materials, EarlyView.This work presents self‐propelled CRISPR/Cas9‐functionalized Au–MRs for rapid, amplification‐free, “on‐the‐fly” DNA detection. By harnessing motion‐assisted signal recovery, the platform achieved the limit of detection in low fM DNA concentrations, enabling detection across a wide dynamic range within only 5 min, which is significantly faster than any ...
Jyoti +3 more
wiley +1 more source
Capturing CDKs in action: Live-cell biosensors pioneer the new frontiers in cell cycle research
Cell Structure and FunctionCyclin-dependent kinases (CDKs) orchestrate cell cycle progression through precise temporal control of substrate phosphorylation. While traditional biochemical approaches and phosphoproteomics have provided valuable insights into CDK-mediated regulation,
Sachiya Nakashima +4 more
doaj +1 more source
Two-dimensional nanomaterials for Förster resonance energy transfer–based sensing applications
Nanophotonics, 2020 Förster resonance energy transfer (FRET)–based sensing has been steadily gaining popularity in the areas of biochemical analysis, environmental monitoring, and disease diagnosis in the past 20 years.
Zhou Jie +3 more
doaj +1 more source
Comparing the performance of mScarlet-I, mRuby3, and mCherry as FRET acceptors for mNeonGreen
bioRxiv, 2019 Förster Resonance Energy Transfer (FRET) has become an immensely powerful tool to profile intra- and inter-molecular interactions. Through fusion of genetically encoded fluorescent proteins (FPs) researchers have been able to detect protein ...
Tyler W. McCullock +2 more
semanticscholar +1 more source
Advanced Functional Materials, EarlyView.An oral nanoplatform, MOP@T@D, which can maintain glucose homeostasis and restore islet β cells in diabetic rats is developed. It achieves efficient intestinal absorption and liver‐targeted delivery. The nanoparticle disintegrates only in response to hyperglycemia to release insulin on demand and provides antioxidant protection through selenoprotein ...
Chenxiao Chu +14 more
wiley +1 more source
Advanced Functional Materials, EarlyView.DNA strands are employed both as dynamic linkers and nanoscale templates for the integration of Ag2S nanoparticles on MoS2, which in turn imparted photothermal responsiveness; this feature permits the selective cargo (fluorophore, quantum dots or an enzyme) release from the MoS2 surface in response to local heat induced by light irradiation.
Kai Chen +3 more
wiley +1 more source
Red-Shifted FRET Biosensors for High-Throughput Fluorescence Lifetime Screening
Biosensors, 2018 We have developed fluorescence resonance energy transfer (FRET) biosensors with red-shifted fluorescent proteins (FP), yielding improved characteristics for time-resolved (lifetime) fluorescence measurements.
Tory M. Schaaf +9 more
doaj +1 more source