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Molecular signaling in live cells studied by FRET
SPIE Proceedings, 2012Genetically encoded biosensors based on fluorescence resonance energy transfer (FRET) enables visualization of signaling events in live cells with high spatiotemporal resolution. We have used FRET to assess temporal and spatial characteristics for signaling molecules, including tyrosine kinases Src and FAK, small GTPase Rac, calcium, and a membrane ...
Shu Chien, Yingxiao Wang
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Characterization of a monolithic device for detection of FRET signals
MRS Proceedings, 2012ABSTRACTFluorescence Resonance Energy Transfer (FRET) is a standard technique used in many medical and biological applications. It involves the detection of transient fluorescent signals coming from the different fluorescent proteins that work in the visible range of the spectrum. Common fluorescent emissions come from the cyan/yellow fluorophores that
P. Louro +4 more
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Localised GPCR signalling as revealed by FRET biosensors
Current Opinion in Cell Biology, 2019Förster resonance energy transfer (FRET) biosensors have provided much evidence for compartmentalised signalling following activation of G protein-coupled receptors (GPCRs). This localised signalling occurs within distinct plasma membrane microdomains and at sub cellular locations including endosomes, mitochondria, Golgi and the nucleus.
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Characterization of fretting related acoustic emission signals
SPIE Proceedings, 2010The acoustic emission (AE) technique is a promising tool for monitoring the integrity of structural members while they are in service. The major obstacle in deploying this technique is the presence of noise from extraneous sources that generate false positives.
Md. Tariqul Alam, Mannur Sundaresan
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Fret Microscopy in Cellular Signal Transduction
1996The study of cellular signal transduction is a rapidly expanding discipline. However, an integrated approach for assessing the behavior of signaling proteins on and in intact cells is lacking. Myriad factors determine the pleiotropic responses of a cell after stimulation.
Philippe I. H. Bastiaens +1 more
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Genetically Encoded FRET Biosensors to Illuminate Compartmentalised GPCR Signalling
Trends in Pharmacological Sciences, 2018Genetically encoded Förster resonance energy transfer (FRET) biosensors have been instrumental to our understanding of how intracellular signalling is organised and regulated within cells. In the last decade, the toolbox, dynamic range and applications of these sensors have expanded beyond basic cell biology applications. In particular, FRET biosensors
Michelle L, Halls, Meritxell, Canals
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Target-assisted FRET signal amplification for ultrasensitive detection of microRNA
The Analyst, 2019The recycling of target miRNA and high quenching efficiency of nanogold greatly improved the sensitivity for miRNA detection.
Bin Wang, Zheng You, Dahai Ren
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Using FRET to analyse signals controlling cell adhesion and migration
Journal of Microscopy, 2012SummaryCell adhesion and migration are dynamic, complex processes that require tight temporal control of signalling cascades at defined subcellular sites to occur. Decades of research have used biochemical methods to identify numerous signalling pathways that are involved in the coordination of adhesion, membrane protrusion and contractility that all ...
Kemp-O'Brien, K., Parsons, M.
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Assessment of cAMP-PKA Signaling in Candida glabrata by FRET-Based Biosensors
2022Fluorescence or Förster resonance energy transfer (FRET)-based biosensors are used to monitor activity through molecular pathways inside the cell. Binding of secondary metabolites or enzyme-guided modification of protein targets can be assessed by quantifying the rate of energy transfer between two adequate fluorophores.
Liesbeth, Demuyser +2 more
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The Signal Parameter for Monitoring Fretting Characteristics in Real-Time
Tribology Transactions, 2012Although the phase difference method, proposed in the previous work by Jeong, can be a very useful signal parameter for investigating fretting behavior as well as distinguishing the fretting regime...
Jin-Seon Kim +3 more
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