Results 61 to 70 of about 1,105,287 (296)
FRET-based peptide screening assay.
, 2018 Cartoon representation of the conjugation of mRuby2-Q-tag and Clover-K-tag, in the presence of bTG, resulting in a cross-linked product. Due to the spectral overlap of mRuby2 and Clover, when in close proximity, excitation of Clover leads to FRET, and ...
Samuel K. Oteng-Pabi (109086) +2 more
core +1 more source
, 2023 In situ monitoring multidrug release in complex cellular microenvironments is significant, and currently, it is still a great challenge. In this work, a smart nanocarrier with the capability of codelivery of small molecules and gene materials as well as ...
Yu Chen (29959) +9 more
core +1 more source
The Shewanella oneidensis Fic enzyme SoFic targets the switch‐I region of EF‐Tu for AMPylation
FEBS Letters, EarlyView.Fic enzymes mediate diverse post‐translational modifications across all domains of life, including AMPylation. Prokaryotic EF‐Tu can be AMPylated and deAMPylated by the conserved Fic enzyme SoFic. Structural and biochemical approaches were used to characterize the effect of AMPylation on EF‐Tu, SoFic's enzymatic activities, and the enzyme‐target ...
Svenja Runge +6 more
wiley +1 more source
Frontiers in Immunology, 2019 The trimeric envelope spikes on the HIV-1 virus surface initiate infection and comprise key targets for antiviral humoral responses. Circulating virions variably present intact envelope spikes, which react with neutralizing antibodies; and altered ...
Krishanu Ray +11 more
doaj +1 more source
FRET-Modulated Multihybrid Nanoparticles for Brightness-Equalized Single-Wavelength Barcoding
, 2019 Semiconductor quantum dots (QDs) are the most versatile fluorophores for Förster resonance energy transfer (FRET) because they can function as both donors and acceptors for a multitude of fluorophores.
Ben Corry (135521) +3 more
core +1 more source
Molecular Oncology, EarlyView.We identify USP29 as the only DUB mirroring CA9 expression, a marker of hypoxia and HIF pathway activation associated with PCA aggressiveness. USP29 stabilizes HIF‐1α and HIF‐2α via a noncanonical mechanism that is independent of PHD/pVHL activity yet relies on proteasomal regulation, establishing USP29 as a previously unrecognized regulator of hypoxic
Amelie S Schober +16 more
wiley +1 more source
FRET-TnC constructs varying the FRET pair of fluorophores and linker length.
, 2016 Five constructs were generated by flanking human cardiac troponin C (cTnC) with one of three FRET pairs of fluorophores. Constructs also differ by the linker length (indicated by number of amino acids, aa) separating the fluorescent proteins from cTnC.
Myriam A. Badr (3219207) +3 more
core +1 more source
Triple Fluorescence Anisotropy Reporter Imaging in Living Cells
Bio-Protocol, 2019 FRET-based genetically encoded biosensors incorporate two fluorescent proteins into their design to enable ratiometric biosensing of signaling activities in live cells.
Brian Ross +4 more
doaj +1 more source
Reprogramming tumor cells with LCOR mRNA nanotherapy to enhance immunotherapy
Molecular Oncology, EarlyView.LCOR (Ligand‐dependent corepressor) mRNA encapsulated in polymeric nanoparticles restores LCOR activity in tumor cells, constraining aggressive tumor cell states and increasing tumor immunogenicity. This reprogramming makes tumor cells more vulnerable to T‐cell attack and enhances their response to immune‐checkpoint immunotherapy.
Gabriel Serra‐Mir +9 more
wiley +1 more source
Nature Communications, 2023 Materials with phototunable full-color circularly polarized luminescence (CPL) have a large storage density, high-security level, and enormous prospects in the field of information encryption and decryption. In this work, device-friendly solid films with
Siyang Lin +5 more
doaj +1 more source