Results 71 to 80 of about 1,105,287 (296)

New Turf for CFP/YFP FRET Imaging of Membrane Signaling Molecules [PDF]

Neuron, 2003
TIRF microscopy can be used in conjunction with CFP/YFP FRET to detect movements of the cytoplasmic tails of GIRK channels (Riven et al., this issue of Neuron). This innovative combination of techniques allows molecular resolution of small motions underlying ion channel activation by G proteins and will likely find widespread use for study of membrane ...
Evans, Jenafer, Yue, David T
openaire   +2 more sources

Single‐molecule DNA flow‐stretch assays for high‐throughput DNA–protein interaction studies

FEBS Open Bio, EarlyView.
We describe an optimised single‐molecule DNA flow‐stretch assay that visualises DNA–protein interactions in real time. Linear DNA fragments are tethered to a surface and stretched by buffer flow for fluorescence imaging. Using λ and φX174 DNA, this protocol enhances reproducibility and accessibility, providing a versatile approach for studying diverse ...
Ayush Kumar Ganguli, Mohammad Nour Alsamsam, Ugnė Bagdonaitė, Van Truc Vu, Chun‐Jen Huang, Polina Kuzhir, Mindaugas Zaremba, Aurimas Kopūstas, Marijonas Tutkus   +8 more
wiley   +1 more source

Targeting FRET-Based Reporters for cAMP and PKA Activity Using AKAP79

Sensors, 2018
Fluorescence resonance energy transfer (FRET)-based sensors for 3′–5′cyclic adenosine monophosphate (cAMP) and protein kinase A (PKA) allow real-time imaging of cAMP levels and kinase activity in intact cells with high spatiotemporal ...
Nshunge Musheshe, Miguel J. Lobo, Martina Schmidt, Manuela Zaccolo   +3 more
doaj   +1 more source

Interbase-FRET binding assay for pre-microRNAs [PDF]

, 2021
The aberrant expression of microRNAs (miRs) has been linked to several human diseases. A promising approach for targeting these anomalies is the use of small-molecule inhibitors of miR biogenesis.
Nilsson, Jesper, Wilhelmsson, Marcus, Edfeldt, Fredrik,, Wypijewska Del Nogal, Anna Wiktoria,, Grotli, Morten,, Grotli, Morten, Lemurell, Malin, Dahlen, Anders,, Wypijewska Del Nogal, Anna Wiktoria, Dahlen, Anders, Nilsson, Jesper,, Lemurell, Malin,, Bood, Mattias, Wilhelmsson, Marcus,, Edfeldt, Fredrik, Bood, Mattias,, Wilhelmsson, L. M., Nilsson, J. R., Dahlen, A., del Nogal, A. W., Edfeldt, F., Lemurell, M., Fredrik Edfeldt, Mattias Bood, Jesper R. Nilsson, L. Marcus Wilhelmsson, Anders Dahlén, Malin Lemurell, Morten Grøtli, Anna Wypijewska del Nogal   +29 more
core   +1 more source

Chasing the signaling run by tri-molecular time-lapse FRET microscopy [PDF]

Cell Death Discovery, 2018
AbstractA feasible design is made to measure three protein/protein interactions to visualize signal pathways by time-lapse Förster resonance energy transfer (FRET) microscopy. When interacting proteins are in close proximity, excitation energy is provided to allow the energy flow from the first molecule to excite the second, followed by energy transfer
Hsiang-Ling Kuo, Pei-Chuan Ho, Shenq-Shyang Huang, Nan-Shan Chang   +3 more
openaire   +4 more sources

Optimizing photoactivation of PA‐mCherry for optical pooled CRISPR screens

FEBS Open Bio, EarlyView.
Photoactivatable PA‐mCherry finds widespread use to optically tag individual cells. However, confocal 405 nm UV laser‐scanning (normal scan) is much less efficient than widefield UV illumination, limiting the use of PA‐mCherry on confocal instruments. We remedy this limitation by reporting that rapid and repeated confocal scanning with a low‐intensity,
Sravasti Mukherjee, Giulia Zanetti, Bram van den Broek, Kees Jalink   +3 more
wiley   +1 more source

Enhancement of Probe Signal for Screening of HIV-1 Protease Inhibitors in Living Cells

Sensors, 2012
The global human immunodeficiency virus infection/acquired immuno-deficiency syndrome (HIV/AIDS) epidemic is one of the biggest threats to human life. Mutation of the virus and toxicity of the existing drugs necessitate the development of new drugs for ...
Huantong Yao, Sha Jin
doaj   +1 more source

It takes two to tango: cardiac fibroblast-derived NO-induced cGMP enters cardiac myocytes and increases cAMP by inhibiting PDE3

Communications Biology, 2023
The occurrence of NO/cGMP signalling in cardiac cells is a matter of debate. Recent measurements with a FRET-based cGMP indicator in isolated cardiac cells revealed NO-induced cGMP signals in cardiac fibroblasts while cardiomyocytes were devoid of these ...
Lukas Menges, Jan Giesen, Kerem Yilmaz, Evanthia Mergia, Annette Füchtbauer, Ernst-Martin Füchtbauer, Doris Koesling, Michael Russwurm   +7 more
doaj   +1 more source

Ligand-binding induced FRET changes between heterodimers.

, 2013
A. A representative image of a cell expressing CXCR4CFP and CCR5YFP that was stimulated with RANTES. The N-FRET image shows marked FRET signals. B. Cells expressing CXCR4CFP and CCR5YFP, or CCR5STAYFP or CCR5Δ4YFP were stimulated with ligands such as ...
Tian Jin (63393), Dale Hereld (376318), Nilgun Isik (376316)   +2 more
core   +1 more source

Ligand regulation of the quaternary organization of cell surface M3 muscarinic acetylcholine receptors analyzed by fluorescence resonance energy transfer (FRET) imaging and homogenous time-resolved FRET [PDF]

, 2010
Flp-In T-REx 293 cells expressing a wild type human M muscarinic acetylcholine receptor construct constitutively and able to express a Receptor Activated Solely by Synthetic Ligand (RASSL) form of this receptor on demand maintained response to the ...
Alvarez-Curto, Elisa, Ward, Richard J., Milligan, Graeme, Pediani, John D.   +3 more
core   +1 more source