Results 51 to 60 of about 1,105,287 (296)

FRETting Mice Shed Light on Cardiac Adrenergic Signaling [PDF]

Circulation Research, 2006
See related article, pages 1084–1091 β-adrenergic receptor (β-AR) signaling in cardiac myocytes influences contractile and relaxation states in the heart. Classically, following hormone activation, β-AR preferentially couples with Gs, which in turn activates adenylyl cyclase and cAMP production.
Lisa M. DiPilato, Jin Zhang
openaire   +1 more source

Pediatric Idiopathic Multicentric Castleman Disease Is Often Severe But Responsive to Siltuximab

Pediatric Blood &Cancer, EarlyView.
ABSTRACT Background Idiopathic multicentric Castleman disease (iMCD) is a potentially fatal immunologic disorder marked by widespread lymphadenopathy and inflammation. Siltuximab, an interleukin‐6 (IL‐6) inhibitor, is the only FDA‐approved treatment for adult patients with iMCD.
Bridget Austin, Sheila K. Pierson, Kelley C. Dacus, Mateo Sarmiento Bustamante, Saishravan Shyamsundar, Larissa Borys, Kayla McHugh, Abiola Irvine, Yoojin Jo, Adam Bagg, Mary Jo Lechowicz, Daisy Alapat, Amy Chadburn, Megan S. Lim, Gordan Srkalovic, Frits van Rhee, David C. Fajgenbaum, Joshua D. Brandstadter   +17 more
wiley   +1 more source

Quantitative Visualization of Intracellular Nanometabolism Using Peak-Shifted Dual-State Emissive FRET Nanoprobes. [PDF]

Adv Sci (Weinh)
This study reveals the long‐elusive intracellular dissolution mechanism of carrier‐free nanomedicines. By utilizing peak‐shifted dual‐state emissive FRET nanoprobes (PDFNPs) that undergo a distinct ratiometric fluorescence peak shift upon disassembly, we achieve real‐time, quantitative tracking of dissolution kinetics in live cells.
Taemaitree F, Sung J, Miki Y, Suzuki R, Koseki Y, Ishii K, Kim M, Tanita K, Sato K, Nakazawa T, Katsube S, Unabara D, Hamaguchi T, Kawakami K, Yonekura K, Hibara A, Wakamiya A, Uji-I H, Kasai H.   +18 more
europepmc   +2 more sources

Investigating cardiac β-adrenergic nuclear signaling with FRET-based biosensors

Annales d'Endocrinologie, 2021
By activating membrane β-adrenergic receptors (β-AR), noradrenaline and adrenaline are the most powerful stimulators of cardiac function. β-ARs are coupled to the synthesis of cAMP, which activates the cAMP-dependent protein kinase (PKA). PKA regulates the key proteins of excitation-contraction coupling but also gene expression.
Vandecasteele, Grégoire, Bedioune, Ibrahim   +1 more
openaire   +3 more sources

Salmonella lipopolysaccharide‐containing supported lipid bilayers as platforms to study bacteriophage interactions

FEBS Letters, EarlyView.
We present robust protocols for the preparation of supported lipid bilayers (SLBs) incorporating either Salmonella smooth LPS or outer membrane vesicles (OMVs). We use a combination of quartz crystal microbalance with dissipation (QCM‐D) and fluorescence microscopy to both characterize the SLBs of various compositions and to probe their interactions ...
Hudson P. Pace, Alyssa Borges, Charles Arnaud, Tove Karlsson, Nina K. Bröker, Stefanie Barbirz, Marta Bally   +6 more
wiley   +1 more source

Structure‐forward targeting of claudins with synthetic binders

FEBS Letters, EarlyView.
Claudins form the paracellular barriers between epithelial and endothelial tissues at tight junctions and are targets for molecular binders with the goal of modulating barrier permeability. Claudin‐binding molecules are relevant in drug delivery or in altering claudin interactions with disease‐causing proteins.
Alex J. Vecchio
wiley   +1 more source

The Rac-FRET Mouse Reveals Tight Spatiotemporal Control of Rac Activity in Primary Cells and Tissues

Cell Reports, 2014
The small G protein family Rac has numerous regulators that integrate extracellular signals into tight spatiotemporal maps of its activity to promote specific cell morphologies and responses.
Anna-Karin E. Johnsson, Yanfeng Dai, Max Nobis, Martin J. Baker, Ewan J. McGhee, Simon Walker, Juliane P. Schwarz, Shereen Kadir, Jennifer P. Morton, Kevin B. Myant, David J. Huels, Anne Segonds-Pichon, Owen J. Sansom, Kurt I. Anderson, Paul Timpson, Heidi C.E. Welch   +15 more
doaj   +1 more source

Autoinducer-Fluorophore Conjugates Enable FRET in LuxR Proteins in Vitro and in Cells [PDF]

, 2022
Cell-to-cell signaling, or quorum sensing (QS), in Gram-negative bacteria is governed by small molecule signals (N-acyl L-homoserine lactones, AHLs) and their cognate intracellular receptors (LuxR-type proteins).
Margaret, McEwan, Emma, Santa, Betty, Slinger, Helen, Blackwell, Matthew, Styles, Michelle, Boursier, Margrith, Mattmann   +6 more
core   +2 more sources

Synergistic perspectives—How single‐molecule biophysics complement biochemical understanding

FEBS Letters, EarlyView.
In this review, we discuss how ensemble biochemistry and single‐molecule approaches are complementary, outline commonly used single‐molecule techniques, and illustrate their relevance through two representative case studies: chromatin organization by SMC complexes and pathway choice during DNA double‐strand break repair.
Sara De Bragança, Eline L. Jansen, Jorine M. Eeftens   +2 more
wiley   +1 more source

Apoptosis Detection in Retinal Ganglion Cells Using Quantitative Changes in Multichannel Fluorescence Colocalization

Biosensors, 2022
KcapTR488 is a dual-fluorophore peptide sensor for the real-time reporting of programmed cell death by fluorescence imaging. KcapTR488 contains a nuclear localization sequence (NLS) conjugated with Texas Red, a caspase-cleavable sequence (DEVD), and a C ...
Xudong Qiu, Seth T. Gammon, James R. Johnson, Federica Pisaneschi, Steven W. Millward, Edward M. Barnett, David Piwnica-Worms   +6 more
doaj   +1 more source